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dc.contributor.authorUtheim, Tor Paaske
dc.contributor.authorUtheim, Øygunn Aass
dc.contributor.authorKhan, Qalbi
dc.contributor.authorSehic, Amer
dc.date.accessioned2017-03-20T14:23:36Z
dc.date.available2017-03-20T14:23:36Z
dc.date.issued2016-03-01
dc.description.abstractThe cornea is critical for normal vision as it allows allowing light transmission to the retina. The corneal epithelium is renewed by limbal epithelial cells (LEC), which are located in the periphery of the cornea, the limbus. Damage or disease involving LEC may lead to various clinical presentations of limbal stem cell deficiency (LSCD). Both severe pain and blindness may result. Transplantation of cultured autologous oral mucosal epithelial cell sheet (CAOMECS) represents the first use of a cultured non-limbal autologous cell type to treat this disease. Among non-limbal cell types, CAOMECS and conjunctival epithelial cells are the only laboratory cultured cell sources that have been explored in humans. Thus far, the expression of p63 is the only predictor of clinical outcome following transplantation to correct LSCD. The optimal culture method and substrate for CAOMECS is not established. The present review focuses on cell culture methods, with particular emphasis on substrates. Most culture protocols for CAOMECS used amniotic membrane as a substrate and included the xenogeneic components fetal bovine serum and murine 3T3 fibroblasts. However, it has been demonstrated that tissue-engineered epithelial cell sheet grafts can be successfully fabricated using temperature-responsive culture surfaces and autologous serum. In the studies using different substrates for culture of CAOMECS, the quantitative expression of p63 was generally poorly reported; thus, more research is warranted with quantification of phenotypic data. Further research is required to develop a culture system for CAOMECS that mimics the natural environment of oral/limbal/corneal epithelial cells without the need for undefined foreign materials such as serum and feeder cells.en_US
dc.description.sponsorshipFunding: Department of Oral Biology, Faculty of Dentistry, University of Oslo and Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.en_US
dc.descriptionSource: <a href=http://dx.doi.org/10.3390/jfb7010005>doi: 10.3390/jfb7010005</a>en_US
dc.identifier.citationUtheim TP, Utheim ØA, Khan KA, Sehic A. Culture of Oral Mucosal Epithelial Cells for the Purpose of Treating Limbal Stem Cell Deficiency. Journal of Functional Biomaterials. 2016en_US
dc.identifier.issn2079-4983
dc.identifier.otherFRIDAID 1346744
dc.identifier.other10.3390/jfb7010005
dc.identifier.urihttp://hdl.handle.net/10037/10790
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalJournal of Functional Biomaterials
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectMedisinsk biologi / Medical biologyen_US
dc.titleCulture of Oral Mucosal Epithelial Cells for the Purpose of Treating Limbal Stem Cell Deficiencyen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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