Vis enkel innførsel

dc.contributor.authorIngebrigtsen, Sveinung Gaarden
dc.contributor.authorSkalko-Basnet, Natasa
dc.contributor.authorHolsæter, Ann Mari
dc.date.accessioned2017-03-11T11:23:20Z
dc.date.available2017-03-11T11:23:20Z
dc.date.issued2016-01-24
dc.description.abstractObjectives: The objective of the present study was to utilize dual asymmetric centrifugation (DAC) as a novel processing approach for the production of liposomes-in-hydrogel formulations. <br>Materials and Methods: Lipid films of phosphatidylcholine, with and without chloramphenicol (CAM), were hydrated and homogenized by DAC to produce liposomes in the form of vesicular phospholipid gels with a diameter in the size range of 200-300 nm suitable for drug delivery to the skin. Different homogenization processing parameters were investigated along with the effect of adding propylene glycol (PG) to the formulations prior to homogenization. The produced liposomes were incorporated into a hydrogel made of 2.5 % (v/v) soluble β-1,3/1,6-glucan (SBG) and mixed by DAC to achieve a homogenous liposomes-in-hydrogel-formulation suitable for topical application. <br>Results and Discussion: CAM-containing liposomes with a vesicle diameter of 282 ± 30 nm and polydispersity index (PI) of 0.13 ± 0.02 were successfully produced by DAC after 50 minutes centrifugation at 3500 rpm, and homogenously (< 4 % content variation) incorporated into the SBG hydrogel. Addition of PG decreased the necessary centrifugation time to 2 minutes and 55 seconds, producing liposomes of 230 ± 51 nm and PI of 0.25 ± 0.04. All formulations had an entrapment efficiency of approximately 50%. <br>Conclusions: We managed to develop a relatively fast and reproducible new method for the production of liposomes-in-hydrogel formulation by DAC.en_US
dc.descriptionManuscript. Published version available at <a href=http://dx.doi.org/10.3109/03639045.2015.1135940> http://dx.doi.org/10.3109/03639045.2015.1135940 </a>en_US
dc.identifier.citationIngebrigtsen SG, Skalko-Basnet N, Holsæter A M. Development and optimization of a new processing approach for manufacturing topical liposomes-in-hydrogel drug formulations by dual asymmetric centrifugation. Drug Development and Industrial Pharmacy. 2016;42(9):1375-1383en_US
dc.identifier.cristinIDFRIDAID 1325395
dc.identifier.doi10.3109/03639045.2015.1135940
dc.identifier.issn0363-9045
dc.identifier.issn1520-5762
dc.identifier.urihttps://hdl.handle.net/10037/10581
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.relation.journalDrug Development and Industrial Pharmacy
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectChloramphenicolen_US
dc.subjectdual asymmetric centrifugationen_US
dc.subjecthydrogelsen_US
dc.subjectliposomesen_US
dc.subjectskin therapyen_US
dc.subjectsoluble beta-glucanen_US
dc.titleDevelopment and optimization of a new processing approach for manufacturing topical liposomes-in-hydrogel drug formulations by dual asymmetric centrifugationen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel