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dc.contributor.authorAndersen, Kasper L.
dc.contributor.authorBeckert, Bertrand
dc.contributor.authorMasquida, Benoît
dc.contributor.authorJohansen, Steinar Daae
dc.contributor.authorNielsen, Henrik
dc.date.accessioned2017-03-17T12:36:35Z
dc.date.available2017-03-17T12:36:35Z
dc.date.issued2016-10-31
dc.description.abstractGroup I introns in nuclear ribosomal RNA of eukaryotic microorganisms are processed by splicing or circularization. The latter results in formation of full-length circular introns without ligation of the exons and has been proposed to be active in intron mobility. We applied qRT-PCR to estimate the copy number of circular intron RNA from the myxomycete Didymium iridis . In exponentially growing amoebae, the circular introns are nuclear and found in 70 copies per cell. During heat-shock, the circular form is up-regulated to more than 500 copies per cell. The intron harbours two ribozymes that have the potential to linearize the circle. To understand the structural features that maintain circle integrity, we performed chemical and enzymatic probing of the splicing ribozyme combined with molecular modeling to arrive at models of the inactive circular form and its active linear counterpart. We show that the two forms have the same overall structure but differ in key parts, including the catalytic core element P7 and the junctions at which reactions take place. These differences explain the relative stability of the circular species, demonstrate how it is prone to react with a target molecule for circle integration and thus supports the notion that the circular form is a biologically significant molecule possibly with a role in intron mobility.en_US
dc.description.sponsorship<br>The Danish Council for Independent Research.</br> <br>The Lundbeck Foundation.</br>en_US
dc.descriptionSource:<a href=http://www.mdpi.com/1420-3049/21/11/1451/htm>doi:10.3390/molecules21111451</a>en_US
dc.identifier.citationAndersen, Beckert B, Masquida B, Johansen S.D., Nielsen H. Accumulation of stable full-length circular group i intron RNAs during heat-shock. Molecules. 2016;21:1451(11)en_US
dc.identifier.cristinIDFRIDAID 1417210
dc.identifier.doi10.3390/molecules21111451
dc.identifier.issn1420-3049
dc.identifier.urihttps://hdl.handle.net/10037/10766
dc.language.isoengen_US
dc.publisherMDPI. Moleculesen_US
dc.relation.journalMolecules
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.subjectgroup I intronen_US
dc.subjectDidymium iridisen_US
dc.subjectcircular RNAen_US
dc.subjecthorizontal gene transferen_US
dc.subjectmolecular modelingen_US
dc.subjectRNA catalysisen_US
dc.titleAccumulation of stable full-length circular group i intron RNAs during heat-shocken_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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