English
norskBrowsing Artikler, rapporter og annet (kjemi) by Author "Altermark, Bjørn"
Now showing items 1-15 of 15
-
Biochemical and structural characterisation of a family GH5 cellulase from endosymbiont of shipworm P. megotara
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-04-04)Background Cellulases play a key role in the enzymatic conversion of plant cell-wall polysaccharides into simple and economically relevant sugars. Thus, the discovery of novel cellulases from exotic biological niches is of great interest as they may present properties that are valuable in the biorefning of lignocellulosic biomass. Results We have characterized a glycoside hydrolase 5 (GH5) domain ... -
Biochemical characterization of ParI, an orphan C5-DNA methyltransferase from Psychrobacter arcticus 273-4
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-05-25)<p>Cytosine-specific DNA methyltransferases are important enzymes in most living organisms. In prokaryotes, most DNA methyltransferases are members of the type II restriction-modification system where they methylate host DNA, thereby protecting it from digestion by the accompanying restriction endonucleases. DNA methyltransferases can also act as solitary enzymes having important roles in controlling ... -
Bioprospecting around Arctic islands: Marine bacteria as rich source of biocatalysts
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-12-11)We have investigated the biotechnological potential of Arctic marine bacteria for their ability to produce a broad spectrum of cold-active enzymes. Marine bacteria exhibiting these features are of great interest for both fundamental research and industrial applications. Macrobiota water and sediment samples have been collected during 2010 and 2011expeditions around the Lofoten and Svalbard islands. ... -
Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-01-19)Background: The use of metagenomics in enzyme discovery constitutes a powerful approach to access to genomes of unculturable community of microorganisms and isolate novel valuable biocatalysts for use in a wide range of biotechnological and pharmaceutical fields. Results: Here we present a novel esterase gene (lip3) identified by functional screening of three fosmid metagenomic libraries, ... -
Complete genome sequence of Halomonas sp. R5-57
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016)The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, Halomonas sp. R5-57 exhibits high salt tolerance over a wide range of temperatures and has extra-cellular hydrolytic activities with several ... -
The crystal structure of the tetrameric DABA-aminotransferase EctB, a rate-limiting enzyme in the ectoine biosynthesis pathway
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-29)L‐2,4‐diaminobutyric acid (DABA) aminotransferases can catalyze the formation of amines at the distal ω‐position of substrates, and is the intial and rate‐limiting enzyme in the biosynthesis pathway of the cytoprotecting molecule (S)‐2‐methyl‐1,4,5,6‐tetrahydro‐4‐pyrimidine carboxylic acid (ectoine). Although there is an industrial interest in the biosynthesis of ectoine, the DABA aminotransferases ... -
Features and structure of a cold active N-acetylneuraminic acid lyase
(Journal article; Peer reviewed, 2019-06-11)N-acetylneuraminate lyases (NALs) are enzymes that catalyze the reversible cleavage and synthesis of sialic acids. They are therefore commonly used for the production of these high-value sugars. This study presents the recombinant production, together with biochemical and structural data, of the NAL from the psychrophilic bacterium Aliivibrio salmonicida LFI1238 (AsNAL). Our characterization shows ... -
High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-20)Background: Several members of the bacterial Halomonadacea family are natural producers of polyhydroxyalkanoates (PHA), which are promising materials for use as biodegradable bioplastics. Type-strain species of Cobetia are designated PHA positive, and recent studies have demonstrated relatively high PHA production for a few strains within this genus. Industrially relevant PHA producers may therefore ... -
High quality draft genome sequence of Streptomyces sp. strain AW19M42 isolated from a sea squirt in Northern Norway
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
A molecular phylogeny of wood-borers (Teredinidae) from Japanese Tsunami Marine Debris
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02)The family Teredinidae (shipworms) contains 70-plus species of boring bivalves specialized to live in and digest wood. Traditional means of species identification and taxonomy of this group encounter numerous challenges, often compounded by the diverse and dynamic nature of shipworm ecology and distribution. Modern integrative taxonomic methods are shedding new light on this complex group, from ... -
Properties and distribution of a metallo-β-lactamase (ALI-1) from the fish pathogen Aliivibrio salmonicida LFI1238
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015)Objectives. To characterize the chromosome-encoded metallo-β-lactamase (MBL) from the psychrophilic, marine fish-pathogenic bacterium Aliivibrio salmonicida LFI1238 and check for the presence of the gene in other Aliivibrio isolates both connected to the fish-farming industry and from the environment. Methods. The MBL gene was cloned and intracellularly expressed in Escherichia coli. Kinetic ... -
Sequence analysis of nonulosonic acid biosynthetic gene clusters in Vibrionaceae and Moritella viscosa
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-20)Nonulosonic acid (NulO) biosynthesis in bacteria is directed by <i>nab</i> gene clusters that can lead to neuraminic, legionaminic or pseudaminic acids. Analysis of the gene content from a set mainly composed of <i>Aliivibrio salmonicida</i> and <i>Moritella viscosa</i> strains reveals the existence of several unique nab clusters, for which the NulO products were predicted. This prediction method ... -
Species diversity and abundance of shipworms (Mollusca: Bivalvia: Teredinidae) in woody marine debris generated by the Great East Japan Earthquake and Tsunami of 2011
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02)The Tohoku tsunami of March 2011 ejected a vast amount of debris into the Pacific Ocean. Wood boring shipworms (Bivalvia: Teredinidae) were either already present in, or settled on, the wooden fraction of this debris, offering a unique opportunity to study shipworm diversity in rafted wood of a known origin and time of ocean entry. Lumber and other wood began appearing on Central Pacific (Hawaiian ... -
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-05-06)Polyhydroxyalkanoates (PHAs) are natural biodegradable polyesters that are produced by numerous prokaryotic microorganisms primarily as a carbon- and energy reserve. The PhaC enzyme catalyzes the last step in the PHA biosynthesis pathway and synthesizes PHA polymers from hydroxyalkanoic acids. A type I PhaC from a PHAproducing marine bacterium Brevundimonas sp. KH11J01 (BrPhaC) was identified, ...
