Blar i forfatter Artikler, rapporter og annet (kjemi) "Lian, Kjersti"
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Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase
Pan, Jolyn; Lian, Kjersti; Sarre, Aili; Leiros, Hanna-Kirsti Schrøder; Williamson, Adele Kim (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-09-21)DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure. The smallest members of this enzyme class carry out their functions with pared-down protein scaffolds comprising only the core catalytic domains. Here we use sequence similarity network analysis of minimal DNA ligases from all biological ... -
Crystal structure of the DNA polymerase III β subunit (β-clamp) from the extremophile Deinococcus radiodurans
Niiranen, Laila; Lian, Kjersti; Johnson, Kenneth A; Moe, Elin (Journal article; Tidsskriftartikkel, 2015-02-27)Background: Deinococcus radiodurans is an extremely radiation and desiccation resistant bacterium which can tolerate radiation doses up to 5,000 Grays without losing viability. We are studying the role of DNA repair and replication proteins for this unusual phenotype by a structural biology approach. The DNA polymerase III β subunit (β-clamp) acts as a sliding clamp on DNA, promoting the binding ... -
Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site
Wissuwa, Juliane; Stokke, Runar; Fedøy, Anita-Elin; Lian, Kjersti; Smalås, Arne O.; Steen, Ida Helene (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-02-24)Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent ... -
Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns
Lian, Kjersti; Furulund, Betty Martine Normann; Tveita, Anders Aune; Haugen, Peik; Johansen, Steinar Daae (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-10-08)Background: Mobile group I introns encode homing endonucleases that confer intron mobility initiated by a double-strand break in the intron-lacking allele at the site of insertion. Nuclear ribosomal DNA of some fungi and protists contain mobile group I introns harboring His-Cys homing endonuclease genes (HEGs). An intriguing question is how protein-coding genes embedded in nuclear ribosomal DNA ... -
MutT from the fish pathogen Aliivibrio salmonicida is a cold active nucleotide pool sanitization enzyme with an unexpected high thermostability
Lian, Kjersti; Leiros, Hanna-Kirsti S.; Moe, Elin (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)