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    • Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida 

      Williamson, Adele Kim; Pedersen, Hege (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-05)
      The genome of the psychrophilic fish-pathogen Aliivibrio salmonicida encodes a putative ATP-dependent DNA ligase in addition to a housekeeping NAD-dependent enzyme. In order to study the structure and activity of the ATP dependent ligase in vitro we have undertaken its recombinant production and purification from an Escherichia coli based expression system. Expression and purification of this ...