• Aminoglycoside resistance in clinical Gram-negative isolates from Norway 

      Haldorsen, Bjørg Christina (Master thesis; Mastergradsoppgave, 2011-04-06)
      Aminoglycosides represent an important class of antimicrobial agents. The prevalence of aminoglycoside resistance among Gram-negative bacteria in Norway is low, but an increased prevalence among clinical isolates of Escherichia coli has been observed during the last years. The most prevalent resistance mechanism is aminoglycoside modifying enzymes. In addition, resistance may occur when bacteria ...
    • Analysis of glomerular target structures for nephritic anti-dsDNA antibodies playing central roles in lupus nephritis 

      Tømmerås, Berit (Master thesis; Mastergradsoppgave, 2008-05)
      Systemic lupus erythematosus is an autoimmune disease characterized by the presence of autoantibodies towards nuclear antigens. Sub-populations of anti-dsDNA antibodies have the potential to bind in the kidney and thereby induce nephritis, but there is no definite distinction that separates non-pathogenic from pathogenic antibodies. There must be parameters that determine anti-dsDNA antibody binding, ...
    • The antioxidant system of arctic seabirds and the effect of HOCs on antioxidant capacity of kidney tissue 

      Jansen, Marta Karoline (Master thesis; Mastergradsoppgave, 2010-06-15)
      The arctic is exposed to a wide range of pollutants which originate from industrialized areas located in temperate regions. Halogenated organic contaminants (HOCs) are of special concern due to their persistency and ability to bioaccumulate. Consequently, animals high in the food chain, such as marine seabirds, are prone to accumulate high levels of these compounds. The HOCs are known to increase ...
    • The biological cost of genetic elements. 

      Munthali, Tamara Tracy (Master thesis; Mastergradsoppgave, 2010-03-15)
      The emergence and increase of antimicrobial resistance among bacterial populations has raised interest in the factor that contributes to this situation. Mobile genetic elements and in particular integrons have been found to play a role in the spread of resistance genes due to their ability to capture and integrate one or more gene cassettes by site-specific recombination. The genes can be integrated ...
    • Characterization of ATG8 Family Protein Binding to TRIM23 and TRIM31 

      Isaksen, Heidi (Master thesis; Mastergradsoppgave, 2018-05-15)
      Macroautophagy (hereafter called autophagy) is a process where proteins, organelles and intracellular micro-organisms are degraded by the lysosome. The autophagosome engulfs a part of the cytoplasm where the cargo is, and transports it to the endosomal-lysosomal system for degradation. Autophagy can also be selective, where cargos are recognized directly by the autophagy receptors or by other proteins ...
    • Characterization of the Arabidopsis thaliana homologue of NBR1 

      Svenning, Steingrim (Master thesis; Mastergradsoppgave, 2009-05-15)
      It is now well established that p62 and NBR1 are selectively degraded by autophagy and can act as cargo receptors or adaptors for the autophagic degradation of ubiquitinated substrates. Research on autophagy in plants is also well under way, but the mechanism by which target substrates are sequestered for autophagic degradation has not been elucidated. The uncharacterized plant protein Q9SB64 shares ...
    • Cyclic nucleotide dependent ATPase activity in inside out vesicles from human erythrocytes 

      Rafiq, Sarah (Master thesis; Mastergradsoppgave, 2015-01-27)
      The transport of cyclic nucleotides out of the cell is energy requiring, dependent on ATP- hydrolysis. The ability to stimulate this ATPase is a hallmark of substrates for ABC-transporters e.g. ABCC5, ABCC4. It is believed that ABCC5 transporter has selective high affinity for cGMP. Previous studies support the idea that ABCC5 contribute to cGMP transport by human erythrocytes. Human erythrocyte ...
    • Development of an assay for determining PDE5 enzyme activity and for screening of potential inhibitors of the PDE5 enzyme in cancer cells 

      Granaas, Magnus Løkke (Master thesis; Mastergradsoppgave, 2013-05-15)
      PDE5 is a member of the superfamily of phosphodiesterases, and it is identified as the main mechanism for breakdown of cGMP in mammals. Sildenafil is a well-known inhibitor of the PDE5 enzyme and it is also shown that sildenafil inhibits the ABCC5 transporter pump. ABCC5 is a member of the superfamily of ABC-transporters, and identified as an important transporter for mediating the cellular efflux ...
    • Development of new tissue culture protocols for enrichment of CD4 T cells associated with neonatal alloimmune thrombocytopenia 

      Killie, Ida Løken (Master thesis; Mastergradsoppgave, 2010-11-15)
      Neonatal alloimmune thrombocytopenia (NAIT) is most commonly caused by destruction of foetal platelets by maternal antibodies reactive to human platelet antigen (HPA)-1a. The activation of antigen-primed B cells to differentiate to antibody-secreting plasma cells usually requires help from CD4 T cells. The strong association between anti-HPA-1a-production and the MHC allele HLA-DRB3*0101 supports ...
    • Directed evolution of Escherichia coli LacZ gene to create diversity in glycosidic bonds hydrolysis 

      Bohra, Pallavi (Master thesis; Mastergradsoppgave, 2011-05-16)
      Starting with LacZ of Escherichia coli, coding for β-galactosidase, the aim of the thesis project is to apply in vitro directed evolution techniques to help create other glycosidic bond hydrolysis activities. This was done using the main β-galactosidase backbone with limited amino acid sequence change. Any altered glycosyl hydrolase activity would lead to changed substrate specificity. Moreover, ...
    • Downstream gene expression of wild type p53 tumor suppressor gene versus mutated and null p53 

      Aljabri, Mohammad Belal (Master thesis; Mastergradsoppgave, 2006-11)
      P53 is a key tumor suppressor and transcription factor protecting us from cancer. The wild type p53 protein functions as a regulatory protein, triggering a variety of cellular responses to different signals. Activation of p53 can lead to cell division arrest, DNA repair, or apoptosis. More than 60% of all human cancers contain p53 mutations. P53 is also reported in many studies to play a role in the ...
    • The effect of oral uptake of nicotine on skin blood perfusion of the face and hands in snus users as determined by thermography. 

      Høiland, Ina Isabella (Master thesis; Mastergradsoppgave, 2014-05-15)
      In recent years the number of people using cigarettes in Norway has significantly declined, with intake of nicotine being replaced by smokeless tobacco (ST) products such as snus placed in the oral cavity. While health risks from smoking cigarettes are well known little is known about health risks of using ST. The main aim of this thesis was to compare the effects of oral use of snus with nicotine ...
    • The effect of sub-lethal concentration of ciprofloxacin on the transfer of multidrug resistance plasmids, fitness costs on the host and the stability of the newly acquired plasmids 

      Mwansa, Besa James (Master thesis; Mastergradsoppgave, 2014-09-30)
      The increase in reported cases of antimicrobial resistance has raised a lot of concerns in the public health community. This has prompted efforts aimed at finding out factors that have been contributing to this situation. In Gram-negative bacteria plasmids have been singled out as the most important vehicles behind the spread of antimicrobial resistance. Some plasmids can harbour several resistance ...
    • Effect of viral early proteins, mutations and IL-17F on the transcriptional activity of the Merkel cell polyomavirus promoter in different cell lines 

      Abdulsalam, Ibrahim Afolabi (Master thesis; Mastergradsoppgave, 2016-05-23)
      Merkel cell polyomavirus (MCPyV) is common in the human population with a seropositivity of approximately 60%. The virus is chronically shed from healthy skin, but the genuine host cell remains unknown and a permissive cell culture system is lacking. The viral genome is in an episomal state in cells where MCPyV has been found. The virus is not harmful in healthy individuals, but it is involved in ...
    • Effects of exogenous hydrogen sulfide administration on cardiac function and reactive oxygen species production : a study in hearts from normal rats and rats with heart hypertrophy or ischemia 

      Paunas, Teodora Ioana (Master thesis; Mastergradsoppgave, 2011-09)
      Coronary heart disease is the leading cause of death worldwide. Infarct size can be limited by interventions used after the ischemic event like the use of thrombolytic therapy or primary percutaneous coronary intervention. Paradoxically, however, the return of blood flow can also result in additional cardiac damage and complications, referred to as reperfusion injury. It has been shown that reperfusion ...
    • Et eksperimentelt studium av kreftceller fra livmorhals - Strålesensitivitet, effektutvikling og cGMP nivåer 

      Livik, Marit (Master thesis; Mastergradsoppgave, 2008-06)
      I denne oppgaven ble kreftceller fra livmorhals bestrålt med gammastråler. Strålingens effekt på celletetthet, celledød og cellesyklus ble undersøkt for å si noe om disse cellenes strålesensitivitet. Den ekstracellulære konsentrasjonen av cGMP ble også målt. Dette sykliske nukleotidet har relevans fordi det har vist seg at nivået av cGMP går ned etter behandling hos pasienter med livmorhalskreft.
    • Hereditary breast and ovarian cancer. Diversity of genetic causes of HBOC in a Norwegian breast and ovarian cancer patient cohort, BRCA2 c.8331+2C>T - a Norwegian founder mutation 

      Skarsfjord, Siri Hermansen (Master thesis; Mastergradsoppgave, 2017-05-14)
      Hereditary breast and ovarian cancer (HBOC) causes 5-10 % of breast cancer cases and 25 % of ovarian cancer cases. About 24 % of HBOC are caused by deleterious variants in BRCA1 and BRCA2. Currently, more than 25 different genes have been associated with HBOC, including BRCA1 and BRCA2, many of which encode proteins participating in homologous recombination repair (HRR) and mismatch repair (MMR). ...
    • Human articular chondrocytes express Chemerin receptor, ChemR23, which conveys inflammatory signalling 

      Berg, Vivian (Master thesis; Mastergradsoppgave, 2008-12-17)
      Common features of arthritis include destruction of extracellular matrices in cartilage and bone as a result of chronic inflammation. Cartilage deterioration is generally described as a result from the effect of immune cells and their inflammatory mediators. However, recent reports suggest a role of chondrocytes in the initiation of inflammation in joints, and that they play a pivotal role in the ...
    • Identification, isolation and characterisation of scavenging endothelial cells in placenta 

      Mjølsnes, Nina (Master thesis; Mastergradsoppgave, 2014-05-15)
      Haemopoiesis occurs in the BM, where haemopoietic stem cells (HSC) reside in a three-dimensional “niche” and give rise to the millions of circulating blood cells required everyday of life. The precise regulation of this immense task occurs by interaction of HSC with other BM cells and extracellular matrix molecules. In addition, the BM vasculature has also now been identified as a key HSC regulator. ...
    • In vitro reconstitution of proMatrix Metalloproteinase-9/Chondroitin Sulfate Proteoglycan Complexes. Identification of motifs in proMMP-9 and the serglycin core protein involved in the complex formation. 

      Dawadi, Rangita (Master thesis; Mastergradsoppgave, 2014-05-15)
      Previously it has been shown that different monocytic leukemic cell lines such as THP-1, MonoMac and U-937 can produce proteolytic enzymes such as MMP-9 as well as various types of proteoglycans (PG). These proteins have been shown to be involved in homeostasis as well as in various diseases, such as cancer. When proMMP-9 was mixed with isolated PGs from these three cell lines or pure serglycin and ...