• Molecular and biochemical characterisation of naturally occurring hyperexpressed and mutated extended spectrum AmpC beta-lactamases in Norwegian clinical isolates of Escherichia coli 

      Igeltjørn, Lise (Master thesis; Mastergradsoppgave, 2009-11-17)
      Jeg har karakterisert kliniske E. coli isolater, med nedsatt følsomhet for cefalosporiner, på nukleotid og amino syre nivå.
    • Molecular characterization of Norwegian clinical isolates of Escherichia coli hyperproducing the chromosomal AmpC beta-lactamase : a regional spread of an IS911-mediated blaAmpC-hyperexpressing ST131 clone 

      Ramberg, Cathrine Caspersen (Master thesis; Mastergradsoppgave, 2012-05-15)
      The worldwide dissemination of antimicrobial resistance is a growing problem causing increased morbidity, mortality, and financial costs. β-lactams are an important family of antimicrobial agents and accounts for ~46% of the total antibiotic use for systemic infections in Norway. Resistance to β-lactams can be caused by several factors where the production of enzymes, β-lactamases, is the major ...
    • A molecular genetic study investigating the role of maternal and placental laeverin gene mutation and fetal whole genome copy number variations in the pathophysiology of preeclampsia 

      Olsen, Hanne Listau (Master thesis; Mastergradsoppgave, 2011-11-29)
      Summary Preeclampsia is a clinical syndrome affecting only pregnant women and is defined as new onset of hypertension and proteinuria after 20 weeks of gestation. Approximately, 2-7 % of all pregnant women in the developed world are affected by this condition and it is a major cause of maternal and fetal morbidity and mortality. Despite intensive research the pathophysiology of preeclampsia is not ...
    • A molecular study of putative pili structures in Enterococuus faecium 

      Schei, Ingvild Kristin Midtervoll (Master thesis; Mastergradsoppgave, 2008-12)
      Infections caused by Enterococcus faecium have increased over the last few decades. In this thesis there has been an attempt to identify putative pili proteins in E. faecium. The putative genes were ligated into a pENTR vector; the insert was sequenced to check the orientation, sequence, length and ends. Then the putative genes were recombined into a pDEST vector for expression. The expression showed ...
    • Oncolytic Activity and Mechanisms of Action by a small β2,2-amino acid derivative LTX-401 

      Mauseth, Brynjar (Master thesis; Mastergradsoppgave, 2015-05-11)
      Antimicrobial peptides are part of the innate immune defence of many organisms and represent a novel class of therapeutics due to their broad-spectrum activities, including cytotoxic activity against cancer cells. It has recently been reported that small antimicrobial β2,2-amino acid derivatives (Mw<500), such as LTX-401, possess potent anticancer activity against Ramos human Burkitt’s lymphoma ...
    • The oncoproteins gelsolin, periostin and thrombospondin are enriched in Merkel cell carcinoma exosomes, and their promoter activity is stimulated by Large T-antigen of Merkel cell polyomavirus 

      Furuholmen-Jenssen, Ida Sofie (Master thesis; Mastergradsoppgave, 2017-05-14)
      Merkel cell carcinoma (MCC) is a rare, aggressive neuroendocrine form of skin cancer with high mortality rate and increasing incidence. Around 80 % of MCC tumours are positive for Merkel cell polyomavirus (MCPyV), the only polyomavirus known to induce cancer in humans. Continuous expression of the viral oncogenes LT-ag and st-ag is necessary for MCPyV positive MCC tumour cell survival, suggesting ...
    • Partial characterization of predicted ABCC5 inhibitors by the aid of human erythrocyte inside-out vesicles 

      Ørvoll, Elin (Master thesis; Mastergradsoppgave, 2011-06-24)
      ABCC5 is a member of the superfamily of ABC-transporters, and it has been identified as an efflux transporter of cGMP. This transporter is also involved in export of antibiotic and cytostatic drugs from target cells, and as such represents a challenge in treatment of cancer and infectious diseases. In order to find inhibitors to ABCC5 mediated drug efflux, compounds predicted as potent inhibitors ...
    • Prevalence of plasmid-mediated quinolone resistance in Norwegian and Swedish clinical isolates of Escherchia coli and Klebsiella spp. 

      Karah, Nabil (Master thesis; Mastergradsoppgave, 2008-06-12)
      The aim of this study was to examine the presence of plasmid-mediated qnr and aac(6')-Ib-cr resistance genes among Escherichia coli and Klebsiella spp. clinical isolates obtained between 2003 and 2005 from Norway and Sweden and showed resistance and/or reduced susceptibility to nalidixic acid and ciprofloxacin, respectively. Prevalence of the qnr (1.6%) and aac(6’)-Ib-cr (14.1%) determinants was ...
    • Production and purification of recombinant C-terminal truncated pro-Matrix Metalloprotease-9 

      Rahman, Fatema (Master thesis; Mastergradsoppgave, 2016-06-21)
      MMP-9 consists of N-terminal signal peptide, a pro-domain, catalytic domain, a long flexible hinge region and C- terminal hemopexin domain. In the present study a truncated version of proMMP-9 which lacking hinge region and C-terminal hemopexin domain was produced. To do so Sf9 insect cells and the BaculoDirect Baculovirus expression system were used. Using site directed mutagenesis, single mutation ...
    • Real-time polymerase chain reaction species specific for Neisseria Gonorrhoeae 

      Olsen, Merethe Elise (Master thesis; Mastergradsoppgave, 2008-05)
      Gonorrhea is a sexually transmitted disease caused by bacteria called gonococci. The disease was described in ancient times, but until the late nineteenth century it was mixed up with syphilis. Eventually microscopy could differ between the organisms and give a reliable diagnosis. Human is the only known reservoir for gonorrhea. It is transferred during all acts of sexual activity, and an infection ...
    • The role of MMP-2 and MMP-14 in cell migration and invasion 

      Okoli, Ndidiamaka Chinelo (Master thesis; Mastergradsoppgave, 2014-02-03)
      Cancer is becoming a global epidemic in recent times and can affect different organs in the body and even more worrying is that they do not have a common cause. Several factors have been implicated in the onset and spread of cancers, one of which are the metalloproteinases (MMPs). The MMPs utilize the same strategy they employ in normal physiological processes in pathology, thus creating a dilemma ...
    • Role of the mannose receptor in tumor associated macrophages. Studies in a mouse melanoma model 

      Rønningen, Iselin (Master thesis; Mastergradsoppgave, 2014-04-15)
      Tumor-associated macrophages (TAMs) play an important role in cancer-related inflammation. TAMs develop M2 polarization which may support tumor growth. Mannose receptor (MR) expression is a hallmark for this cell-subpopulation, and the MR has recently been suggested to be involved in TAM polarization. The aim of the study was to investigate the role of MR in TAM polarization and tumor development ...
    • Study of interaction between BK virus large T-antigen and agnoprotein 

      Adou, Koman Mireille Sophie Chinan (Master thesis; Mastergradsoppgave, 2011-08)
      Human polyomavirus BK (BKV) is a non enveloped virus with a double-stranded, circular DNA genome. BKV infects >70% of the human population world-wide. Infection occurs predominantly during childhood and the virus remains in a latent state throughout life in the immune competent individuals. In the context of immunosuppression, however, reactivation occurs and can lead to renal stenosis and interstitial ...
    • A study of possible genetic causes of inherited breast and ovarian cancer in a Norwegian cancer population 

      Jarhelle, Elisabeth (Master thesis; Mastergradsoppgave, 2013-05-15)
      Homologous recombination repair (HRR) is an important repair mechanism, and mutations disrupting the function of this machinery might contribute to cancer formation. Several proteins interact in this mechanism, and the two best known are BRCA1 and BRCA2. Mutations in their corresponding genes BRCA1 and BRCA2 are found in 40% of hereditary breast and ovarian cancer cases. However, there are still ...
    • A study of the interaction between MAPKAP Kinase 5 / MK5 and DNAJB1 

      Lægreid, Kari Jenssen (Master thesis; Mastergradsoppgave, 2011-05-02)
      The mitogen activated protein kinases (MAPK) are a large and diverse family of protein kinases, contributing to the cells ability to respond to external stimuli by relaying messages in a well orchestrated way until they reach their final destinations. This is achieved through successive phosphorylation events. One member of this large family is mitogen activated protein kinase activated protein ...
    • A study of the protein kinase MK5's effect on melanoma cell proliferation 

      Dumitriu, Gianina Aurica (Master thesis; Mastergradsoppgave, 2012-09-14)
      The mitogen-activated protein kinases (MAPKs) play a crucial role in cellular processes such as proliferation, differentiation, apoptosis, metabolism and motility. Both conventional and atypical MAPKs can phosphorylate other proteins referred to as MAPK- activated protein kinases (MAPKAPK) such as MK5 or p38-regulated /activated protein kinase. Mutations in the MK5 gene have been detected in lung, ...
    • Theoretical and experimental studies of thermolysin inhibition 

      Wuxiuer, Yimingjiang (Master thesis; Mastergradsoppgave, 2008-05-15)
      Zinc-metalloproteinases play a key role in the biosythesis and metabolism of different bioactive peptides. As a member of zinc-metalloproteinases, thermolysin has served as a model system to study the inhibition mechanism of other metalloproteinases. Inhibitors of thermolysin have considerable potential as therapeutic agents. In the present master thesis, docking calculations were performed and ...
    • The world of β-glucans : a review of biological roles, applications and potential areas of research 

      Ali, Syed Haris (Master thesis; Mastergradsoppgave, 2009-05-15)
      Among many known and tested immunomodulators, polysaccharides isolated from various natural sources occupy a prominent position. An important group of these polysaccharides is represented by the homopolymers of β-glucose, called β-glucans. Their very low-to-negligible toxicity and immunomodulating effects show the promise it has in the therapy of a variety of infectious and cancer illnesses. ...