Now showing items 1-10 of 12
Analysis of glomerular target structures for nephritic anti-dsDNA antibodies playing central roles in lupus nephritis
(Master thesis; Mastergradsoppgave, 2008-05)
Systemic lupus erythematosus is an autoimmune disease characterized by the presence of autoantibodies towards nuclear antigens. Sub-populations of anti-dsDNA antibodies have the potential to bind in the kidney and thereby induce nephritis, but there is no definite distinction that separates non-pathogenic from pathogenic antibodies. There must be parameters that determine anti-dsDNA antibody binding, ...
Real-time polymerase chain reaction species specific for Neisseria Gonorrhoeae
(Master thesis; Mastergradsoppgave, 2008-05)
Gonorrhea is a sexually transmitted disease caused by bacteria called gonococci. The disease was described in ancient times, but until the late nineteenth century it was mixed up with syphilis. Eventually microscopy could differ between the organisms and give a reliable diagnosis. Human is the only known reservoir for gonorrhea. It is transferred during all acts of sexual activity, and an infection ...
A molecular study of putative pili structures in Enterococuus faecium
(Master thesis; Mastergradsoppgave, 2008-12)
Infections caused by Enterococcus faecium have increased over the last few decades. In this thesis there has been an attempt to identify putative pili proteins in E. faecium. The putative genes were ligated into a pENTR vector; the insert was sequenced to check the orientation, sequence, length and ends. Then the putative genes were recombined into a pDEST vector for expression. The expression showed ...
Et eksperimentelt studium av kreftceller fra livmorhals - Strålesensitivitet, effektutvikling og cGMP nivåer
(Master thesis; Mastergradsoppgave, 2008-06)
I denne oppgaven ble kreftceller fra livmorhals bestrålt med gammastråler. Strålingens effekt på celletetthet, celledød og cellesyklus ble undersøkt for å si noe om disse cellenes strålesensitivitet. Den ekstracellulære konsentrasjonen av cGMP ble også målt. Dette sykliske nukleotidet har relevans fordi det har vist seg at nivået av cGMP går ned etter behandling hos pasienter med livmorhalskreft.
Prevalence of plasmid-mediated quinolone resistance in Norwegian and Swedish clinical isolates of Escherchia coli and Klebsiella spp.
(Master thesis; Mastergradsoppgave, 2008-06-12)
The aim of this study was to examine the presence of plasmid-mediated qnr and aac(6')-Ib-cr resistance genes among Escherichia coli and Klebsiella spp. clinical isolates obtained between 2003 and 2005 from Norway and Sweden and showed resistance and/or reduced susceptibility to nalidixic acid and ciprofloxacin, respectively. Prevalence of the qnr (1.6%) and aac(6’)-Ib-cr (14.1%) determinants was ...
Molecular and biochemical characterisation of naturally occurring hyperexpressed and mutated extended spectrum AmpC beta-lactamases in Norwegian clinical isolates of Escherichia coli
(Master thesis; Mastergradsoppgave, 2009-11-17)
Jeg har karakterisert kliniske E. coli isolater, med nedsatt følsomhet for cefalosporiner, på nukleotid og amino syre nivå.
The 5-HT1E receptor. Receptor structure and ligand interactions.
(Master thesis; Mastergradsoppgave, 2006-12-08)
5-HT (5-Hydroxytryptamine; Serotonin) is the neurotransmitter with the highest number of known receptors in humans, and the importance of 5-HT is also reflected in the number of species using 5-HT as a signalling molecule and the number of 5-HT receptors found within each species. 15 human 5-HT receptors have at this date been characterised and classified into seven 5-HT receptor families. Six of ...
Downstream gene expression of wild type p53 tumor suppressor gene versus mutated and null p53
(Master thesis; Mastergradsoppgave, 2006-11)
P53 is a key tumor suppressor and transcription factor protecting us from cancer. The wild type p53 protein functions as a regulatory protein, triggering a variety of cellular responses to different signals. Activation of p53 can lead to cell division arrest, DNA repair, or apoptosis. More than 60% of all human cancers contain p53 mutations. P53 is also reported in many studies to play a role in the ...
Theoretical and experimental studies of thermolysin inhibition
(Master thesis; Mastergradsoppgave, 2008-05-15)
Zinc-metalloproteinases play a key role in the biosythesis and metabolism of different bioactive peptides. As a member of zinc-metalloproteinases, thermolysin has served as a model system to study the inhibition mechanism of other metalloproteinases. Inhibitors of thermolysin have considerable potential as therapeutic agents. In the present master thesis, docking calculations were performed and ...
Human articular chondrocytes express Chemerin receptor, ChemR23, which conveys inflammatory signalling
(Master thesis; Mastergradsoppgave, 2008-12-17)
Common features of arthritis include destruction of extracellular matrices in cartilage and bone as a result of chronic inflammation. Cartilage deterioration is generally described as a result from the effect of immune cells and their inflammatory mediators. However, recent reports suggest a role of chondrocytes in the initiation of inflammation in joints, and that they play a pivotal role in the ...