Now showing items 1-10 of 12
Et eksperimentelt studium av kreftceller fra livmorhals - Strålesensitivitet, effektutvikling og cGMP nivåer
(Master thesis; Mastergradsoppgave, 2008-06)
I denne oppgaven ble kreftceller fra livmorhals bestrålt med gammastråler. Strålingens effekt på celletetthet, celledød og cellesyklus ble undersøkt for å si noe om disse cellenes strålesensitivitet. Den ekstracellulære konsentrasjonen av cGMP ble også målt. Dette sykliske nukleotidet har relevans fordi det har vist seg at nivået av cGMP går ned etter behandling hos pasienter med livmorhalskreft.
Prevalence of plasmid-mediated quinolone resistance in Norwegian and Swedish clinical isolates of Escherchia coli and Klebsiella spp.
(Master thesis; Mastergradsoppgave, 2008-06-12)
The aim of this study was to examine the presence of plasmid-mediated qnr and aac(6')-Ib-cr resistance genes among Escherichia coli and Klebsiella spp. clinical isolates obtained between 2003 and 2005 from Norway and Sweden and showed resistance and/or reduced susceptibility to nalidixic acid and ciprofloxacin, respectively. Prevalence of the qnr (1.6%) and aac(6’)-Ib-cr (14.1%) determinants was ...
Real-time polymerase chain reaction species specific for Neisseria Gonorrhoeae
(Master thesis; Mastergradsoppgave, 2008-05)
Gonorrhea is a sexually transmitted disease caused by bacteria called gonococci. The disease was described in ancient times, but until the late nineteenth century it was mixed up with syphilis. Eventually microscopy could differ between the organisms and give a reliable diagnosis. Human is the only known reservoir for gonorrhea. It is transferred during all acts of sexual activity, and an infection ...
Downstream gene expression of wild type p53 tumor suppressor gene versus mutated and null p53
(Master thesis; Mastergradsoppgave, 2006-11)
P53 is a key tumor suppressor and transcription factor protecting us from cancer. The wild type p53 protein functions as a regulatory protein, triggering a variety of cellular responses to different signals. Activation of p53 can lead to cell division arrest, DNA repair, or apoptosis. More than 60% of all human cancers contain p53 mutations. P53 is also reported in many studies to play a role in the ...
The world of β-glucans : a review of biological roles, applications and potential areas of research
(Master thesis; Mastergradsoppgave, 2009-05-15)
Among many known and tested immunomodulators, polysaccharides isolated from various natural sources occupy a prominent position. An important group of these polysaccharides is represented by the homopolymers of β-glucose, called β-glucans. Their very low-to-negligible toxicity and immunomodulating effects show the promise it has in the therapy of a variety of infectious and cancer illnesses. ...
A molecular study of putative pili structures in Enterococuus faecium
(Master thesis; Mastergradsoppgave, 2008-12)
Infections caused by Enterococcus faecium have increased over the last few decades. In this thesis there has been an attempt to identify putative pili proteins in E. faecium. The putative genes were ligated into a pENTR vector; the insert was sequenced to check the orientation, sequence, length and ends. Then the putative genes were recombined into a pDEST vector for expression. The expression showed ...
Theoretical and experimental studies of thermolysin inhibition
(Master thesis; Mastergradsoppgave, 2008-05-15)
Zinc-metalloproteinases play a key role in the biosythesis and metabolism of different bioactive peptides. As a member of zinc-metalloproteinases, thermolysin has served as a model system to study the inhibition mechanism of other metalloproteinases. Inhibitors of thermolysin have considerable potential as therapeutic agents. In the present master thesis, docking calculations were performed and ...
The 5-HT1E receptor. Receptor structure and ligand interactions.
(Master thesis; Mastergradsoppgave, 2006-12-08)
5-HT (5-Hydroxytryptamine; Serotonin) is the neurotransmitter with the highest number of known receptors in humans, and the importance of 5-HT is also reflected in the number of species using 5-HT as a signalling molecule and the number of 5-HT receptors found within each species. 15 human 5-HT receptors have at this date been characterised and classified into seven 5-HT receptor families. Six of ...
Human articular chondrocytes express Chemerin receptor, ChemR23, which conveys inflammatory signalling
(Master thesis; Mastergradsoppgave, 2008-12-17)
Common features of arthritis include destruction of extracellular matrices in cartilage and bone as a result of chronic inflammation. Cartilage deterioration is generally described as a result from the effect of immune cells and their inflammatory mediators. However, recent reports suggest a role of chondrocytes in the initiation of inflammation in joints, and that they play a pivotal role in the ...
Characterization of the Arabidopsis thaliana homologue of NBR1
(Master thesis; Mastergradsoppgave, 2009-05-15)
It is now well established that p62 and NBR1 are selectively degraded by autophagy and can act as cargo receptors or adaptors for the autophagic degradation of ubiquitinated substrates. Research on autophagy in plants is also well under way, but the mechanism by which target substrates are sequestered for autophagic degradation has not been elucidated. The uncharacterized plant protein Q9SB64 shares ...