Now showing items 1-10 of 76
Analysis of glomerular target structures for nephritic anti-dsDNA antibodies playing central roles in lupus nephritis
(Master thesis; Mastergradsoppgave, 2008-05)
Systemic lupus erythematosus is an autoimmune disease characterized by the presence of autoantibodies towards nuclear antigens. Sub-populations of anti-dsDNA antibodies have the potential to bind in the kidney and thereby induce nephritis, but there is no definite distinction that separates non-pathogenic from pathogenic antibodies. There must be parameters that determine anti-dsDNA antibody binding, ...
In vitro reconstitution of proMatrix Metalloproteinase-9/Chondroitin Sulfate Proteoglycan Complexes. Identification of motifs in proMMP-9 and the serglycin core protein involved in the complex formation.
(Master thesis; Mastergradsoppgave, 2014-05-15)
Previously it has been shown that different monocytic leukemic cell lines such as THP-1, MonoMac and U-937 can produce proteolytic enzymes such as MMP-9 as well as various types of proteoglycans (PG). These proteins have been shown to be involved in homeostasis as well as in various diseases, such as cancer. When proMMP-9 was mixed with isolated PGs from these three cell lines or pure serglycin and ...
The oncoproteins gelsolin, periostin and thrombospondin are enriched in Merkel cell carcinoma exosomes, and their promoter activity is stimulated by Large T-antigen of Merkel cell polyomavirus
(Master thesis; Mastergradsoppgave, 2017-05-14)
Merkel cell carcinoma (MCC) is a rare, aggressive neuroendocrine form of skin cancer with high mortality rate and increasing incidence. Around 80 % of MCC tumours are positive for Merkel cell polyomavirus (MCPyV), the only polyomavirus known to induce cancer in humans. Continuous expression of the viral oncogenes LT-ag and st-ag is necessary for MCPyV positive MCC tumour cell survival, suggesting ...
Role of the mannose receptor in tumor associated macrophages. Studies in a mouse melanoma model
(Master thesis; Mastergradsoppgave, 2014-04-15)
Tumor-associated macrophages (TAMs) play an important role in cancer-related inflammation. TAMs develop M2 polarization which may support tumor growth. Mannose receptor (MR) expression is a hallmark for this cell-subpopulation, and the MR has recently been suggested to be involved in TAM polarization. The aim of the study was to investigate the role of MR in TAM polarization and tumor development ...
Real-time polymerase chain reaction species specific for Neisseria Gonorrhoeae
(Master thesis; Mastergradsoppgave, 2008-05)
Gonorrhea is a sexually transmitted disease caused by bacteria called gonococci. The disease was described in ancient times, but until the late nineteenth century it was mixed up with syphilis. Eventually microscopy could differ between the organisms and give a reliable diagnosis. Human is the only known reservoir for gonorrhea. It is transferred during all acts of sexual activity, and an infection ...
A molecular study of putative pili structures in Enterococuus faecium
(Master thesis; Mastergradsoppgave, 2008-12)
Infections caused by Enterococcus faecium have increased over the last few decades. In this thesis there has been an attempt to identify putative pili proteins in E. faecium. The putative genes were ligated into a pENTR vector; the insert was sequenced to check the orientation, sequence, length and ends. Then the putative genes were recombined into a pDEST vector for expression. The expression showed ...
Molecular characterization of Norwegian clinical isolates of Escherichia coli hyperproducing the chromosomal AmpC beta-lactamase : a regional spread of an IS911-mediated blaAmpC-hyperexpressing ST131 clone
(Master thesis; Mastergradsoppgave, 2012-05-15)
The worldwide dissemination of antimicrobial resistance is a growing problem causing increased morbidity, mortality, and financial costs. β-lactams are an important family of antimicrobial agents and accounts for ~46% of the total antibiotic use for systemic infections in Norway. Resistance to β-lactams can be caused by several factors where the production of enzymes, β-lactamases, is the major ...
Et eksperimentelt studium av kreftceller fra livmorhals - Strålesensitivitet, effektutvikling og cGMP nivåer
(Master thesis; Mastergradsoppgave, 2008-06)
I denne oppgaven ble kreftceller fra livmorhals bestrålt med gammastråler. Strålingens effekt på celletetthet, celledød og cellesyklus ble undersøkt for å si noe om disse cellenes strålesensitivitet. Den ekstracellulære konsentrasjonen av cGMP ble også målt. Dette sykliske nukleotidet har relevans fordi det har vist seg at nivået av cGMP går ned etter behandling hos pasienter med livmorhalskreft.
A study of the protein kinase MK5's effect on melanoma cell proliferation
(Master thesis; Mastergradsoppgave, 2012-09-14)
The mitogen-activated protein kinases (MAPKs) play a crucial role in cellular processes such as proliferation, differentiation, apoptosis, metabolism and motility. Both conventional and atypical MAPKs can phosphorylate other proteins referred to as MAPK- activated protein kinases (MAPKAPK) such as MK5 or p38-regulated /activated protein kinase. Mutations in the MK5 gene have been detected in lung, ...
Partial characterization of predicted ABCC5 inhibitors by the aid of human erythrocyte inside-out vesicles
(Master thesis; Mastergradsoppgave, 2011-06-24)
ABCC5 is a member of the superfamily of ABC-transporters, and it has been identified as an efflux transporter of cGMP. This transporter is also involved in export of antibiotic and cytostatic drugs from target cells, and as such represents a challenge in treatment of cancer and infectious diseases. In order to find inhibitors to ABCC5 mediated drug efflux, compounds predicted as potent inhibitors ...