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dc.contributor.authorBakke-Mckellep, AM
dc.contributor.authorSperstad, Sigmund
dc.contributor.authorPenn, MH
dc.contributor.authorSalas, PM
dc.contributor.authorRefstie, Ståle
dc.contributor.authorLandsverk, Tor
dc.contributor.authorRingø, Einar
dc.contributor.authorKrogdahl, Åshild
dc.date.accessioned2009-09-10T11:33:59Z
dc.date.available2009-09-10T11:33:59Z
dc.date.issued2007
dc.description.abstractSoyabean meal (SBM)-induced enteritis in the distal intestine of the teleost Atlantic salmon (Salmo salar L.) and other salmonids may be considered a model for diet-related mucosal disorders in other animals and man. The role of the intestinal microbiota in its pathogenesis was explored. Compared to diets containing fishmeal (FM) as the sole protein source, responses to extracted SBM or the prebiotic inulin, with or without oxytetracycline (OTC) inclusion, were studied following a 3-week feeding trial. Intestinal microbiota, organosomatic indices and histology, as well as immunohistochemical detection of proliferating cell nuclear antigen (PCNA), heat shock protein 70 (HSP70) and caspase-3-positive cells in the distal intestine, were studied. Distal intestine somatic indices (DISI) were higher in inulin and lower in SBM compared to FM-fed fish. The low DISI caused by SBM corresponded with histological changes, neither of which was affected by OTC, despite a significant decrease in adherent bacteria count. Image analysis of PCNA-stained sections showed a significant increase in the proliferative compartment length in SBM-fed fish, accompanied by apparent increases in reactivity to HSP70 and caspase-3 along the mucosal folds, indicating induction of cellular repair and apoptosis, respectively. Fish fed the SBM diet had higher total number as well as a more diverse population composition of adherent bacteria in the distal intestine. Thus SBM-induced enteritis is accompanied by induction of distal intestinal epithelial cell protective responses and changes in microbiota. Putative involvement of bacteria in the inflammatory response merits further investigation.en
dc.descriptionThis is the publishers version/PDF (institutional repository or PubMed Central can publish publishers version after 12 month embargo)en
dc.format.extent2478869 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationBritish Journal of Nutrition 97(2007) s. 699-713 DOI: 10.1017/S0007114507381397en
dc.identifier.cristinIDFRIDAID 393604
dc.identifier.issn0007-1145
dc.identifier.urihttps://hdl.handle.net/10037/2103
dc.identifier.urnURN:NBN:no-uit_munin_1854
dc.language.isoengen
dc.publisherCambridge University Pressen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923en
dc.subjectInflammationen
dc.subjectEnteritisen
dc.subjectMucosal disorderen
dc.subjectDieten
dc.subjectIntestineen
dc.titleEffects of dietary soybean meal, inulin and oxytetracycline on gastrointestinal histological characteristics, distal intestine cell proliferation and intestinal microbiota in Atlantic salmon (Salmo salar L.)en
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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