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dc.contributor.authorChang, Chia Jung
dc.contributor.authorRobertsen, Camilla
dc.contributor.authorSun, Baojian
dc.contributor.authorRobertsen, Børre
dc.date.accessioned2022-05-24T08:47:21Z
dc.date.available2022-05-24T08:47:21Z
dc.date.issued2014-07-02
dc.description.abstractn this work we have tested the in vivo antiviral activity of type I interferons (IFNs) in Atlantic salmon by injecting presmolts intramuscularly (i.m.) with plasmids encoding IFNa1, IFNb or IFNc under the control of a CMV promoter, and measured expression of antiviral genes in organs and protection against infection with infectious salmon anemia virus (ISAV)infection. Allthree IFN plasmids induced expression of antiviral genes (Mx, Viperin, ISG15 and IFIT5) at the muscle injection site while the control plasmid had little effect. Only IFNb and IFNc plasmids induced expression of antiviral genes in head kidney, liver and heart. This suggests that IFNb and IFNc are distributed systemically while IFNa1 is active only at the injection site. Injection of IFNc plasmid was found to induce expression of antiviral genes and receptors for virus RNA (RIG-I, TLR3 and TLR7) in head kidney from 1 to at least 8 weeks. Immunoblotting showed increased expression of ISG15 and Mx protein in liver with time during this time period. Challenge of presmolts with ISAV8 weeks after injection ofIFN plasmids, showed strong protection ofthe IFNc plasmid injected fish, low protection of the IFNb plasmid injected fish and no protection of the IFNa1 plasmid injected fish. Clues to the difference in protection obtained with IFNb and IFNc plasmids were found by immunohistochemical and immunoblot studies of Mx protein, which indicated that IFNc plasmid stimulated stronger Mx protein expression in heart tissues and liver endothelial cells than IFNb plasmid. Taken together,these data suggestthati.m. injection ofthe IFNc expression plasmid may be a new method for protecting Atlantic salmon against virus infection.en_US
dc.identifier.citationChang CJ, Robertsen C, Sun B, Robertsen B. Protection of Atlantic salmon against virus infection by intramuscular injection of IFNc expression plasmid. Vaccine. 2014;32(36):4695-4702en_US
dc.identifier.cristinIDFRIDAID 1148757
dc.identifier.doi10.1016/j.vaccine.2014.05.059
dc.identifier.issn0264-410X
dc.identifier.issn1873-2518
dc.identifier.urihttps://hdl.handle.net/10037/25266
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalVaccine
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2014 The Author(s)en_US
dc.titleProtection of Atlantic salmon against virus infection by intramuscular injection of IFNc expression plasmiden_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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