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dc.contributor.authorCioni, Lara
dc.contributor.authorNikiforov, Vladimir
dc.contributor.authorMiranda Fernandes Coelho, Ana Carolina
dc.contributor.authorSandanger, Torkjel M
dc.contributor.authorHerzke, Dorte
dc.date.accessioned2022-11-29T09:59:34Z
dc.date.available2022-11-29T09:59:34Z
dc.date.issued2022-11-23
dc.description.abstractPer- and polyfluoroalkyl substances (PFAS) are a class of chemicals including over 4700 substances. As a limited number of PFAS is routinely analyzed in human serum, complementary analytical methods are required to characterize the overlooked fraction. A promising tool is the total oxidizable precursors (TOP) assay to look for precursors by oxidation to perfluoroalkyl acids (PFAA). The TOP assay was originally developed for large volumes of water and had to be adapted for 250 μL of human serum. Optimization of the method was performed on serum samples spiked with model precursors. Oxidative conditions similar to previous TOP assay methods were not sufficient for complete oxidation of model precursors. Prolonged heating time (24 h) and higher oxidant amount (95 mg of Na2S2O8 per 225 μL of serum) were needed for complete conversion of the model precursors and accomplishing PFAA yields of 35–100 %. As some precursors are not fully converted to PFAA, the TOP assay can only provide semi-quantitative estimates of oxidizable precursors in human serum. However, the TOP assay can be used to give indications about the identity of unknown precursors by evaluating the oxidation products, including perfluoroalkyl sulfonic acids (PFSA) and perfluoroalkyl ether carboxylic acids (PFECA). The optimized TOP assay for human serum opens the possibility for high-throughput screening of human serum for undetected PFAA precursors.en_US
dc.identifier.citationCioni L, Nikiforov V, Miranda Fernandes Coelho AC, Sandanger TM, Herzke D. Total Oxidizable Precursors Assay for PFAS in Human Serum. Environment International. 2022en_US
dc.identifier.cristinIDFRIDAID 2081479
dc.identifier.doi10.1016/j.envint.2022.107656
dc.identifier.issn0160-4120
dc.identifier.issn1873-6750
dc.identifier.urihttps://hdl.handle.net/10037/27580
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofCioni, L. (2023). Human Exposure to PFAS and Other Anthropogenic Organofluorine Chemicals in Tromsø between 1986 and 2015. (Doctoral thesis). <a href=https://hdl.handle.net/10037/31818>https://hdl.handle.net/10037/31818</a>.
dc.relation.journalEnvironment International
dc.relation.projectIDEC/H2020: 860665en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleTotal Oxidizable Precursors Assay for PFAS in Human Serumen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)