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Analysis of glomerular target structures for nephritic anti-dsDNA antibodies playing central roles in lupus nephritis
(Master thesis; Mastergradsoppgave, 2008-05)
Systemic lupus erythematosus is an autoimmune disease characterized by the presence of autoantibodies towards nuclear antigens. Sub-populations of anti-dsDNA antibodies have the potential to bind in the kidney and thereby induce nephritis, but there is no definite distinction that separates non-pathogenic from pathogenic antibodies. There must be parameters that determine anti-dsDNA antibody binding, ...
In vitro reconstitution of proMatrix Metalloproteinase-9/Chondroitin Sulfate Proteoglycan Complexes. Identification of motifs in proMMP-9 and the serglycin core protein involved in the complex formation.
(Master thesis; Mastergradsoppgave, 2014-05-15)
Previously it has been shown that different monocytic leukemic cell lines such as THP-1, MonoMac and U-937 can produce proteolytic enzymes such as MMP-9 as well as various types of proteoglycans (PG). These proteins have been shown to be involved in homeostasis as well as in various diseases, such as cancer. When proMMP-9 was mixed with isolated PGs from these three cell lines or pure serglycin and ...
The oncoproteins gelsolin, periostin and thrombospondin are enriched in Merkel cell carcinoma exosomes, and their promoter activity is stimulated by Large T-antigen of Merkel cell polyomavirus
(Master thesis; Mastergradsoppgave, 2017-05-14)
Merkel cell carcinoma (MCC) is a rare, aggressive neuroendocrine form of skin cancer with high mortality rate and increasing incidence. Around 80 % of MCC tumours are positive for Merkel cell polyomavirus (MCPyV), the only polyomavirus known to induce cancer in humans. Continuous expression of the viral oncogenes LT-ag and st-ag is necessary for MCPyV positive MCC tumour cell survival, suggesting ...
Role of the mannose receptor in tumor associated macrophages. Studies in a mouse melanoma model
(Master thesis; Mastergradsoppgave, 2014-04-15)
Tumor-associated macrophages (TAMs) play an important role in cancer-related inflammation. TAMs develop M2 polarization which may support tumor growth. Mannose receptor (MR) expression is a hallmark for this cell-subpopulation, and the MR has recently been suggested to be involved in TAM polarization. The aim of the study was to investigate the role of MR in TAM polarization and tumor development ...
Studies of genes potentially involved in the induction of flowering in Festuca pratensis (Huds.)
(Master thesis; Mastergradsoppgave, 2006-06-08)
Many plants in northern regions require a period of low temperatures and short days (called vernalisation) during the winter season to initiate the flowering process. In this experiment, some genes thought to be involved in vernalisation response and induction of flowering in meadow fescue (Festuca pratensis) has been investigated. The genes were chosen based on their possible contribution in the ...
A study of regulatory small RNAs in Vibrio salmonicida: construction of a knock-out mutant and a cDNA library
(Master thesis; Mastergradsoppgave, 2008-06-01)
The marine fish pathogen Vibrio salmonicida is the causative agent for vibriosis in
Atlantic salmon (Salmo salar L.), Rainbow trout (Oncorhynchus mykiss) and Atlantic cod
(Gadus morhua L.). V. salmonicidas virulence is regulated by Quorum sensing (QS)
systems, which includes important regulatory small RNAs (sRNAs). sRNAs have the last
years been identified in large numbers, and mostly in pathogenic ...
Real-time polymerase chain reaction species specific for Neisseria Gonorrhoeae
(Master thesis; Mastergradsoppgave, 2008-05)
Gonorrhea is a sexually transmitted disease caused by bacteria called gonococci. The disease was described in ancient times, but until the late nineteenth century it was mixed up with syphilis. Eventually microscopy could differ between the organisms and give a reliable diagnosis. Human is the only known reservoir for gonorrhea. It is transferred during all acts of sexual activity, and an infection ...
Regulation of prostanoid effects in whole blood : immediate-early anti-inflammatory effects of prostaglandin E2
(Master thesis; Mastergradsoppgave, 2008-06-01)
To understand the regulation of the pathophysiological processes, such as inflammation, thrombosis and atherosclerosis, it is very important to characterize the interactions between circulating cells and which molecules that contributes to and promote these interactions. As a part of this, the role of eicosanoids in cell-cell interactions in whole blood ex vivo and isolated blood cells are investigated. ...
A molecular study of putative pili structures in Enterococuus faecium
(Master thesis; Mastergradsoppgave, 2008-12)
Infections caused by Enterococcus faecium have increased over the last few decades. In this thesis there has been an attempt to identify putative pili proteins in E. faecium. The putative genes were ligated into a pENTR vector; the insert was sequenced to check the orientation, sequence, length and ends. Then the putative genes were recombined into a pDEST vector for expression. The expression showed ...
Molecular characterization of Norwegian clinical isolates of Escherichia coli hyperproducing the chromosomal AmpC beta-lactamase : a regional spread of an IS911-mediated blaAmpC-hyperexpressing ST131 clone
(Master thesis; Mastergradsoppgave, 2012-05-15)
The worldwide dissemination of antimicrobial resistance is a growing problem causing increased morbidity, mortality, and financial costs. β-lactams are an important family of antimicrobial agents and accounts for ~46% of the total antibiotic use for systemic infections in Norway. Resistance to β-lactams can be caused by several factors where the production of enzymes, β-lactamases, is the major ...