• The anticancer activity of lytic peptides is inhibited by heparan sulfate on the surface of the tumor cells 

      Fadnes, Bodil Irene; Rekdal, Øystein; Uhlin-Hansen, Lars (Journal article; Tidsskriftartikkel; Peer reviewed, 2009-06-15)
      Background: Cationic antimicrobial peptides (CAPs) with antitumor activity constitute a promising group of novel anticancer agents. These peptides induce lysis of cancer cells through interactions with the plasma membrane. It is not known which cancer cell membrane components influence their susceptibility to CAPs. We have previously shown that CAPs interact with the two glycosaminoglycans (GAGs), ...
    • Biosynthesis of Promatrix Metalloproteinase-9/Chondroitin Sulphate Proteoglycan Heteromer Involves a Rottlerin-Sensitive Pathway 

      Malla, Nabin; Berg, Eli; Moens, Ugo; Uhlin-Hansen, Lars; Winberg, Jan-Olof (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      Background: Previously we have shown that a fraction of the matrix metalloproteinase-9 (MMP-9) synthesized by the macrophage cell line THP-1 was bound to a chondroitin sulphate proteoglycan (CSPG) core protein as a reduction sensitive heteromer. Several biochemical properties of the enzyme were changed when it was bound to the CSPG. <br>Methodology/Principal Findings: By use of affinity chromatography, ...
    • Characterisation and prognostic value of tertiary lymphoid structures in oral squamous cell carcinoma 

      Wirsing, Anna Maria; Rikardsen, Oddveig G; Steigen, Sonja Eriksson; Uhlin-Hansen, Lars; Hadler-Olsen, Elin Synnøve (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-08-23)
      <p><i>Background -</i>Oral squamous cell carcinomas are often heavily infiltrated by immune cells. The organization of B-cells, follicular dendritic cells, T-cells and high-endothelial venules into structures termed tertiary lymphoid structures have been detected in various types of cancer, where their presence is found to predict favourable outcome. The purpose of the present study was to evaluate ...
    • Cleavage of the urokinase receptor (uPAR) on oral cancer cells: Regulation by transforming growth factor - beta1 (TGF-beta1) and potential effects on migration and invasion 

      Magnussen, Synnøve; Hadler-Olsen, Elin Synnøve; Costea, Daniela Elena; Berg, Eli; Cavalcanti Jacobsen, Cristiane de Albuquerque; Mortensen, Bente; Salo, Tuula; Martinez, Inigo Zubiavrre; Winberg, Jan-Olof; Uhlin-Hansen, Lars; Svineng, Gunbjørg (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-05-19)
      Background: Urokinase plasminogen activator (uPA) receptor (uPAR) is up-regulated at the invasive tumour front of human oral squamous cell carcinoma (OSCC), indicating a role for uPAR in tumour progression. We previously observed elevated expression of uPAR at the tumour-stroma interface in a mouse model for OSCC, which was associated with increased proteolytic activity. The tumour microenvironment ...
    • Clinicopathological characteristics of oral squamous cell carcinoma in Northern Norway: a retrospective study 

      Rikardsen, Oddveig G; Bjerkli, Inger-Heidi; Uhlin-Hansen, Lars; Hadler-Olsen, Elin Synnøve; Steigen, Sonja Eriksson (Journal article; Tidsskriftartikkel; Peer reviewed, 2014)
    • A combined histo-score based on tumor differentiation and lymphocytic infiltrate is a robust prognostic marker for mobile tongue cancer 

      Bjerkli, Inger-Heidi; Hadler-Olsen, Elin Synnøve; Nginamau, Elisabeth Sivy; Laurvik, Helene; Søland, Tine Merete; Costea, Daniela Elena; Uhlin-Hansen, Lars; Steigen, Sonja Eriksson (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-30)
      We wanted to evaluate the prognostic value of common histopathological variables in a large cohort of patients with cancer in the mobile tongue as such information can be important for treatment stratification of the individual patient, especially for patients with low-stage disease. In addition, we wanted to investigate whether an alternative scoring model with fewer options would compromise the ...
    • Grading of oral squamous cell carcinomas – Intra and interrater agreeability: Simpler is better? 

      Steigen, Sonja E; Søland, Tine Merete; Nginamau, Elisabeth Sivy; Laurvik, Helene; Costea, Daniela-Elena; Johannessen, Anne Christine; Jebsen, Peter Wilhelm; Bjerkli, Inger-Heidi; Uhlin-Hansen, Lars; Hadler-Olsen, Elin Synnøve (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-01-03)
      <i>Background</i> - Numerous studies have been presented on histological grading of oral squamous cell carcinomas (OSCC) for predicting survival, but uncertainty of their usefulness rises due to discordances of results. A scoring system should be robust and well validated, and intra‐ and interrater agreement can be used as a tool to visualize the strength of reproducibility.<p><p> <i>Methods</i> ...
    • Intracellular MMP-2 Activity in Skeletal Muscle is Associated with Type II Fibers 

      Hadler-Olsen, Elin Synnøve; Solli, Ann Iren; Hafstad, Anne Dragøy; Winberg, Jan-Olof; Uhlin-Hansen, Lars (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-09-29)
      Matrix metalloproteinase 2 (MMP-2) is a proteolytic enzyme implicated in motility, differentiation, and regeneration of skeletal muscle fibers through processing of extracellular substrates. Although MMP-2 has been found to be localized intracellularly in cardiomyocytes where the enzyme is thought to contribute to post-ischemic loss of contractility, little is known about intracellular MMP-2 activity ...
    • Loss of S100A14 expression at the tumor-invading front correlates with poor differentiation and worse prognosis in oral squamous cell carcinoma 

      Pandey, Sushma; Osman, Tarig Al-Hadi; Sharma, Sunita; Vallenari, Evan Michael; Shahdafar, Aboulghassem; Pun, Chin B.; Gautam, Dej K.; Uhlin-Hansen, Lars; Rikardsen, Oddveig G; Johannessen, Anne Christine; Costea, Daniela Elena; Sapkota, Dipak (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-23)
      <i>Background</i> - We previously showed a tumor‐suppressive function of S100A14 in oral squamous cell carcinoma (OSCC). This study aimed to examine the prognostic significance and differentiation‐related function of S100A14 in OSCC.<p><p> <i>Methods</i> - S100A14 expression was examined in 170 OSCCs from Norwegian and Nepalese populations using immunohistochemistry. Pro‐differentiation function ...
    • Macrophages modulate migration and invasion of human tongue squamous cell carcinoma 

      Pirilä, Emma; Väyrynen, Otto; Sundquist, Elias; Päkkilä, Kaisa; Nyberg, Pia; Nurmenniemi, Sini; Pääkkönen, Virve; Pesonen, Paula; Dayan, Dan; Vered, Marilena; Uhlin-Hansen, Lars; Salo, Tuula (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-03-26)
      Oral tongue squamous cell carcinoma (OTSCC) has a high mortality rate and the incidence is rising worldwide. Despite advances in treatment, the disease lacks specific prognostic markers and treatment modality. The spreading of OTSCC is dependent on the tumor microenvironment and involves tumor-associated macrophages (TAMs). Although the presence of TAMs is associated with poor prognosis in OTSCC, ...
    • Plectin as a prognostic marker in non-metastatic oral squamous cell carcinoma 

      Rikardsen, Oddveig G; Magnussen, Synnøve; Svineng, Gunbjørg; Hadler-Olsen, Elin Synnøve; Uhlin-Hansen, Lars; Steigen, Sonja Eriksson (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-08-26)
      <p>Background: Oral squamous cell carcinoma (OSCC) is associated with a poor 5-year survival rate. In general, patients diagnosed with small tumors have a fairly good prognosis, but some small tumors have an aggressive behavior leading to early death. There are at present no reliable prognostic biomarkers for oral cancers. Thus, to optimize treatment for the individual patient, there is a need ...
    • Presence of high-endothelial venules correlates with a favorable immune microenvironment in oral squamous cell carcinoma 

      Wirsing, Anna Maria; Ervik, Ida Korsnes; Seppola, Marit; Uhlin-Hansen, Lars; Steigen, Sonja Eriksson; Hadler-Olsen, Elin Synnøve (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02-07)
      Oral squamous cell carcinomas are associated with a poor prognosis, which may be partly due to functional impairment of the immune response. Lymphocyte recruitment to the tumor site is facilitated by high-endothelial venules, whereas expression of programmed-death ligand 1 (PD-L1) can impair T-cell function. Thus, we hypothesize that these factors are important in shaping the immune response in oral ...
    • Presence of high-endothelial venules correlates with a favorable immune microenvironment in oral squamous cell carsinoma 

      Wirsing, Anna Maria; Ervik, Ida Korsnes; Seppola, Marit; Uhlin-Hansen, Lars; Steigen, Sonja Eriksson; Hadler-Olsen, Elin (Master thesis; Mastergradsoppgave, 2017-10-31)
      Oral squamous cell carcinomas (OSCC) are associated with a poor prognosis, which may be partly due to functional impairment of the immune response. Lymphocyte recruitment to the tumor site is facilitated by high-endothelial venules (HEV), whereas expression of programmed-death ligand 1 (PD-L1) can impair T cell function. Thus, we hypothesize that these factors are important in shaping the immune ...
    • Presence of tumour high-endothelial venules is an independent positive prognostic factor and stratifies patients with advanced-stage oral squamous cell carcinoma 

      Wirsing, Anna Maria; Rikardsen, Oddveig G; Steigen, Sonja Eriksson; Uhlin-Hansen, Lars; Hadler-Olsen, Elin Synnøve (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-09-17)
      Background: Staging of oral squamous cell carcinoma is based on the TNM system, which has been deemed insufficient for prognostic purposes. Hence, better prognostic tools are needed to reflect the biological diversity of these cancers. Previously, high numbers of specialized blood vessels called high-endothelial venules have been reported to be associated with prolonged survival in patients with ...
    • Proteases in plasma and kidney of db /db mice as markers of diabetes-induced nephropathy 

      Hadler-Olsen, Elin Synnøve; Winberg, Jan-Olof; Reinholt, Finn P.; Larsen, Terje; Uhlin-Hansen, Lars; Jenssen, Trond Geir; Berg, Eli; Kolset, Svein Olav (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      Db/db mice are overweight, dyslipidemic and develop diabetic complications, relevant for similar complications in human type 2 diabetes. We have used db/db and db/+ control mice to investigate alterations in proteinase expression and activity in circulation and kidneys by SDS-PAGE zymography, electron microscopy, immunohistochemistry, Western blotting, and in situ zymography. Plasma from db/db mice ...
    • The proteoglycan repertoire of lymphoid cells 

      Fadnes, Bodil Irene; Husebekk, Anne; Svineng, Gunbjørg; Rekdal, Øystein; Yanagishita, Masaki; Kolset, Svein Olav; Uhlin-Hansen, Lars (Journal article; Tidsskriftartikkel; Peer reviewed, 2012)
      Proteoglycans have been studied to a limited extent in lymphoid cells. In this study we have investigated the expression of proteoglycans in B-cells, CD4+ T-cells, CD8+ T-cells, natural killer cells, as well as in nine different cell lines established from patients with lymphoid malignancies. Serglycin was the major proteoglycan expressed at mRNA level by the primary lymphocytes. None of the syndecans ...
    • Small lytic peptides escape the inhibitory effect of heparan sulfate on the surface of cancer cells 

      Fadnes, Bodil Irene; Uhlin-Hansen, Lars; Lindin, Inger; Rekdal, Øystein (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      Several naturally occurring cationic antimicrobial peptides (CAPs), including bovine lactoferricin (LfcinB), display promising anticancer activities. These peptides are unaffected by multidrug resistance mechanisms and have been shown to induce a protective immune response against solid tumors, thus making them interesting candidates for developing novel lead structures for anticancer treatment. ...
    • Stromal impact on tumor growth and lymphangiogenesis in human carcinoma xenografts 

      Rikardsen, Oddveig G; Hadler-Olsen, Elin Synnøve; Steigen, sonja eriksson; Ljones, Hilde; Grenman, Reidar; Kanapathippillai, Premasany; Winberg, Jan-Olof; Svineng, Gunbjørg; Uhlin-Hansen, Lars (Journal article; Tidsskriftartikkel; Peer reviewed, 2010)
    • Tumor budding score predicts lymph node status in oral tongue squamous cell carcinoma and should be included in the pathology report 

      Bjerkli, Inger-Heidi; Laurvik, Helene; Nginamau, Elisabeth Sivy; Søland, Tine Merete; Costea, Daniela Elena; Hov, Håkon; Uhlin-Hansen, Lars; Hadler-Olsen, Elin Synnøve; Steigen, Sonja Eriksson (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-25)
      <i>Background</i> - The majority of oral cavity cancers arise in the oral tongue. The aim of this study was to evaluate the prognostic value of tumor budding in oral tongue squamous cell carcinoma, both as a separate variable and in combination with depth of invasion. We also assessed the prognostic impact of the 8th edition of the American Joint Committee on Cancer’s TNM classification (TNM8), where ...
    • Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes 

      Magnussen, Synnøve; Hadler-Olsen, Elin Synnøve; Latysheva, Nadezhda; Pirila, Emma; Steigen, Sonja Eriksson; Hanes, Robert; Salo, Tuula; Winberg, Jan-Olof; Uhlin-Hansen, Lars; Svineng, Gunbjørg (Journal article; Tidsskriftartikkel; Peer reviewed, 2014)