Blar i forfatter Det helsevitenskapelige fakultet "Werner, Guido"

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    • Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40 

      Zischka, Melanie; Künne, Carsten T.; Blom, Jochen; Wobser, Dominique; Sakιnç, Türkân; Schmidt-Hohagen, Kerstin; Dabrowski, P. Wojtek; Nitsche, Andreas; Hübner, Johannes; Hain, Torsten; Chakraborty, Trinad; Linke, Burkhard; Goesmann, Alexander; Voget, Sonja; Daniel, Rolf; Schomburg, Dietmar; Hauck, Rüdiger; Hafez, Hafez M.; Tielen, Petra; Jahn, Dieter; Solheim, Margrete; Sadowy, Ewa; Larsen, Jesper; Jensen, Lars Bogø; Ruiz-Garbajosa, Patricia; Pérez, Dianelys Quiñones; Mikalsen, Theresa; Bender, Jennifer; Steglich, Matthias; Nübel, Ulrich; Witte, Wolfgang; Werner, Guido (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-03-12)
      Background: Enterococcus faecalis is a multifaceted microorganism known to act as a beneficial intestinal commensal bacterium. It is also a dreaded nosocomial pathogen causing life-threatening infections in hospitalised patients. Isolates of a distinct MLST type ST40 represent the most frequent strain type of this species, distributed worldwide and originating from various sources (animal, human, ...

    • Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis 

      Starikova, Irina; Al-Haroni, Mohammed; Werner, Guido; Roberts, Adam P.; Nielsen, Kåre M.; Johnsen, Pål Jarle (Journal article; Tidsskriftartikkel; Peer reviewed, 2013-07-05)
      Objectives: To determine the fitness effects of various mobile genetic elements (MGEs) in Enterococcus faecium and Enterococcus faecalis when newly acquired. We also tested the hypothesis that the biological cost of vancomycin resistance plasmids could be mitigated during continuous growth in the laboratory.<p> <p>Methods: Different MGEs, including two conjugative transposons (CTns) of the Tn916 ...

    • The global dissemination of hospital clones of Enterococcus faecium 

      van Hal, Sebastiaan J.; Willems, Rob J.L.; Gouliouris, Theodore; Ballard, Susan A.; Coque, Teresa M.; Hammerum, Anette M.; Hegstad, Kristin; Westh, Henrik; Howden, Benjamin P.; Malhotra-Kumar, Surbhi; Werner, Guido; Yanagihara, Katsunori; Earl, Ashlee M.; Raven, Katherine E.; Corander, Jukka; Bowden, Rory (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-30)
      <i>Background</i> - The hospital-adapted A1 group of <i>Enterococcus faecium</i> remains an organism of significant concern in the context of drug-resistant hospital-associated infections. How this pathogen evolves and disseminates remains poorly understood.<br><br> <i>Methods</i> - A large, globally representative collection of short-read genomic data from the hospital-associated A1 group of ...

    • Investigating the mobilome in clinically important lineages of Enterococcus faecium and Enterococcus faecalis 

      Mikalsen, Theresa; Pedersen, Torunn Annie; Willems, Rob J; Coque, Teresa M.; Werner, Guido; Sadowy, Eva; van Schaik, Willem; Jensen, Lars Bogø; Sundsfjord, Arnfinn; Hegstad, Kristin (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-04-10)
      Background: The success of Enterococcus faecium and E. faecalis evolving as multi-resistant nosocomial pathogens is associated with their ability to acquire and share adaptive traits, including antimicrobial resistance genes encoded by mobile genetic elements (MGE). Here, we investigate this mobilome in successful hospital associated genetic lineages, E. faecium sequence type (ST)17 (n=10) and ...

    • The successful uptake and sustainability of rapid infectious disease and antimicrobial resistance point-of-care testing requires a complex 'mix-and-match' implementation package 

      Hays, John P.; Mitsakakis, Konstantinos; Luz, Saturnino; van Belkum, Alex; Becker, Karsten; van den Bruel, Ann; Harbarth, Stephan; Rex, John H.; Simonsen, Gunnar Skov; Werner, Guido; Di Gregori, Valentina; Lüdke, Gerd; van Staa, Tjeerd; Moran-Gilad, Jacob; Bachmann, Till T. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-02)
      The emergence and spread of antimicrobial resistance is one of the major global issues currently threatening the health and wealth of nations, with effective guidelines and intervention strategies urgently required. Such guidelines and interventions should ideally be targeted at individuals, communities, and nations, requiring international coordination for maximum effect. In this respect, the ...

    • Taking hospital pathogen surveillance to the next level 

      Werner, Guido; Couto, Natacha; Feil, Edward J.; Novais, Angela; Hegstad, Kristin; Howden, Benjamin P.; Friedrich, Alexander W.; Reuter, Sandra (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-04-26)
      High-throughput bacterial genomic sequencing and subsequent analyses can produce large volumes of high-quality data rapidly. Advances in sequencing technology, with commensurate developments in bioinformatics, have increased the speed and efficiency with which it is possible to apply genomics to outbreak analysis and broader public health surveillance. This approach has been focused on targeted ...

    • Update on prevalence and mechanisms of resistance to linezolid, tigecycline and daptomycin in enterococci in Europe: towards a common nomenclature 

      Bender, Jennifer; Cattoir, Vincent; Hegstad, Kristin; Sadowy, Eva; Coque, Teresa M.; Westh, Henrik; Hammerum, Anette M.; Schaffer, Kirsten; Burns, Karen; Murchan, Stephen; Novais, Carla; Freitas, Ana R.; Peixe, Luísa; Del Grosso, Maria; Pantosti, Annalisa; Werner, Guido (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-02)
      Vancomycin-resistant enterococci (VRE) are important nosocomial pathogens. Invasive VRE infections are difficult to treat since common therapeutic options including ampicillin and glycopeptides often fail. In vitro, most VRE remain susceptible to last-resort antibiotics such as linezolid, tigecycline and daptomycin. However, neither tigecycline nor linezolid act in a bactericidal manner, and ...