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Effect of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 multiplex assayed cytokines – Consequences for defining reference values in healthy humans
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-06-06)
Cytokines are potentially useful biomarkers of sepsis and other inflammatory conditions. Many cytokines can be released by leukocytes and platelets after sampling. The sampling and processing techniques are consequently critically important to measure the <i>in vivo</i> levels. We therefore examined the effects of four different anticoagulants, EDTA, citrate, lepirudin, heparin compared to serum, ...
Combined inhibition of C5 and CD14 efficiently attenuated the inflammatory response in a porcine model of meningococcal sepsis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-02-27)
Background<br>
Fulminant meningococcal sepsis, characterized by overwhelming innate immune activation, mostly affects young people and causes high mortality. This study aimed to investigate the effect of targeting two key molecules of innate immunity, complement component C5, and co-receptor CD14 in the Toll-like receptor system, on the inflammatory response in meningococcal sepsis.<p>
Methods ...
Gas embolism during surgery. A complement mediated condition?
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-10-01)
<p><i>Introduction</i>: Venous air embolism (VAE) may arise during surgical procedures. VAE might be complicated with a systemic inflammatory response, disseminated intervascular coagulation, multi-organ failure and cardiovascular collapse. During a short time-span, three patients at our institution developed signs of VAE in conjunction with gynecological surgery. One died, one developed severe ...
Bradykinin-Induced Shock Increase Exhaled Nitric Oxide, Complement Activation and Cytokine Production in Pigs
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-03-04)
Bradykinin is an important mediator in blood pressure regulation, ischemic
precondition and capillary leakage, allergy, anaphylaxis, inflammation, and
nociception, at least partly via the generation of nitric oxide (NO). Macrophages
are particularly abundant in the porcine lung circulation. Upon bradykinin binding
macrophages release cytokines and endothelial ...
Combined inhibition of complement and CD14 improved outcome in porcine polymicrobial sepsis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-11-27)
Introduction: Sepsis is an exaggerated and dysfunctional immune response to infection. Activation of innate immunity
recognition systems including complement and the Toll-like receptor family initiate this disproportionate inflammatory
response. The aim of this study was to explore the effect of combined inhibition of the complement component
C5 and the Toll-like receptor co-factor CD14 on survival, ...
Staphylococcus aureus-induced complement activation promotes tissue factor-mediated coagulation
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02-13)
<p><i>Background - </i>There is extensive cross‐talk between the complement system, the Toll‐like receptors (TLRs), and hemostasis. Consumptive coagulopathy is a hallmark of sepsis, and is often mediated through increased tissue factor (TF) expression.
<p><i>Objectives - </i>To study the relative roles of complement, TLRs and TF in <i>Staphylococcus aureus</i>‐induced coagulation.
<p><i>Methods - ...
Open chest and pericardium facilitate transpulmonary passage of venous air emboli
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-17)
<p><i>Background:</i> Transpulmonary passage of air emboli can lead to fatal brain- and myocardial infarctions. We studied whether pigs with open chest and pericardium had a greater transpulmonary passage of venous air emboli than pigs with closed thorax.
<p><i>Methods:</i> We allocated pigs with verified closed foramen ovale to venous air infusion with either open chest with sternotomy and opening ...
Phagocytosis of live and dead Escherichia coli and Staphylococcus aureus in human whole blood is markedly reduced by combined inhibition of C5aR1 and CD14
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-15)
<i>Background</i> - Sepsis is a dysregulated host response to infection. The aim of this study was to investigate the effects of complement- and CD14 inhibition on phagocytosis of live and dead Gram-negative and Gram-positive bacteria in human whole blood.<p>
<p><i>Methods</i> - Lepirudin-anticoagulated blood was incubated with live or dead <i>E. coli</i> or <i>S. aureus</i> at 37 °C for 120 min ...
Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-27)
<i>Introduction</i> - Right ventricular (RV) myocardial dysfunction is a common feature in septic shock. It can worsen outcome, but the etiology is poorly understood. Pulmonary artery hypertension (PAH) plays a part in the pathogenesis of the right heart dysfunction in sepsis but its importance is unknown. In pigs, PAH in sepsis is substantial and the translational value of porcine sepsis models ...
Air Bubbles Activate Complement and Trigger Hemostasis and C3-Dependent Cytokine Release Ex Vivo in Human Whole Blood
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-03)
Venous air embolism, which may complicate medical and surgical procedures, activates complement and triggers
thromboinflammation. In lepirudin-anticoagulated human whole blood, we examined the effect of air bubbles on complement and its
role in thromboinflammation. Whole blood from 16 donors was incubated with air bubbles without or with inhibitors of C3, C5,
C5aR1, or CD14. Complement activation, ...