Blar i tittel Mastergradsoppgaver i marin bioteknologi

Viser treff 21-40 av 40

    • Exploring the potential of two arctic marine bacteria for the production of bioactive metabolites 

      Jenssen, Marte (Master thesis; Mastergradsoppgave, 2017-05-15)
      The number of compounds being isolated from the marine environment is increasing, and there is a great potential for discovering new marine derived drug candidates. Improved collection techniques has strengthened bioprospecting on a wider diversity of marine microorganisms. The focus on microorganisms has led to the realisation that many of the natural products originally isolated from macroorganisms, ...

    • Expression of Secondary Metabolite Gene Clusters and Production of Secondary Metabolites in Three Nostoc strains Subjected to Deprivation on Nutrients and Competition 

      Wilhelmsen, Oda Sofie Bye (Master thesis; Mastergradsoppgave, 2019-05-15)
      Cyanobacteria are a unique source of natural products, where most of them are synthesized by non-ribosomal peptide synthase, polyketide synthase, or a hybrid of these pathways. The identification of such biosynthetic genes responsible for the production of secondary metabolites is still a relatively unexplored area, and it remains many natural products for which a biosynthetic origin is unknown. ...

    • From Lab to Industry - Enzymes from Undifferentiated Marine Rest Raw Material 

      Steinsholm, Silje (Master thesis; Mastergradsoppgave, 2014-05-15)
      The aim of this project was to optimize the extraction of esterase enzymes from undifferentiated herring rest raw material. This was conducted by comparing, in laboratory scale, the outcome of using homogenised and non-homogenised raw material, and by extracting with buffers of different pH. Further on, clarification experiments of flocculation were performed on the optimal extract. The focus was ...

    • Genomic characterization and insights of local adaptation in Norwegian juvenile lumpfish populations 

      Granviken, Emilie Østby (Master thesis; Mastergradsoppgave, 2021-05-18)
      The lumpfish (Cyclopterus lumpus Linnaeus, 1758) has for many years been an attractive target for the roe fisheries in Norway and has more recently become an important cleanerfish in the salmonid farming industry for the control of salmon lice (Copepoda: Caligidae). Despite this, there is a lack of knowledge about several life aspects of the species and studies on its genetic structure have been ...

    • Impact of CRISPR/Cas9 on the expressions of selected immune genes in salmonid cells 

      Palerud, Jocelyn Hernandez (Master thesis; Mastergradsoppgave, 2023-07-05)
      The discovery of CRISPR/Cas9 as a gene editing tool has revolutionized the field of molecular biology due to its huge potential for innovative applications. However, the biosafety concerns on CRISPR/Cas9-mediated gene editing like off-target mutations, triggering of cell death, and induction of cellular stress need to be elucidated. This thesis aims to investigate the impacts of electroporation ...

    • Isolation and characterisation of secondary metabolites from arctic, marine invertebrates 

      Glomsaker, Eirin (Master thesis; Mastergradsoppgave, 2017-05-15)
      Bioprospecting is the systematic search for and discovery of products in nature, with the purpose of developing commercial products. The marine environment displays a rich biological diversity, as well as a diversity within environmental factors. This environment has necessitated the production of potent secondary metabolites by marine organisms in their arms race against predators and pathogens, ...

    • Isolering av etylester og triacylglyserol ved hjelp av fast fase ekstraksjon 

      Guldberg, Mia Brattli (Master thesis; Mastergradsoppgave, 2018-05-15)
      I de siste par tiårene har det vært en kraftig økning i humant bruk av kosttilskudd som inneholder langkjedede omega-3 fettsyrer, det vil si eicosapentaensyre (EPA) og docosaheksaensyre (DHA). Årsaken er de antatte positive helseeffekter disse fettsyrene har. I dag er det vanligst at slike kosttilskudd tas i form av kapsler, blant annet fordi mange mennesker synes marine oljer er ubehagelig å drikke ...

    • Manipulating the Epigenetic Machinery of Marine Fungi Through the Application of Epigenetic Modifiers to Induce the Biosynthesis of Novel Secondary Metabolites 

      Marø, Cathrine Eiken (Master thesis; Mastergradsoppgave, 2020-05-15)
      Fungi are among the most prolific producers of secondary metabolites, many of which have huge utility for society as various anti-infective and anticancer drugs. The genes encoding these secondary metabolites are arranged in continuous biosynthetic gene clusters (BGCs). Each fungus can have tens of different BGCs in its genome, possessing the ability to produce a multitude of secondary metabolites ...

    • Mikrobiell stabilitet og kjemisk sammensetning i hydrolysat fra Calanus finmarchicus 

      Jørstad, Torunn Johansen (Master thesis; Mastergradsoppgave, 2015-05-15)
      Calanus finmarchicus (raudåte) er den mest tallrike av alle marine skalldyrarter, med en stående bestand på cirka 70-80 millioner tonn i det Nordiske hav. Siden ny bærekraftig innhøstingsteknologi er utviklet for arten, er det mulig å utnytte denne ressursen kommersielt. I dag produseres olje fra C. finmarchicus, og har flere dokumenterte helseeffekter. Ved produksjon framstilles fire ganger mer ...

    • Mikrobiota hos grønn kråkebolle, Strongylocentrotus droebachiensis. Fokus på identifikasjon av bakteriestammer 

      Bentdal, Solveig (Master thesis; Mastergradsoppgave, 2017-05-15)
      It has been reported that the green sea urchin, Strongylocentrotus droebachiensis, can be affected by symptoms that is associated with the disease called “bald sea urchin disease”. However, no pathogen for sea urchins are known in the literature, but a working hypothesis claims that species of the Vibrio splendidus clade can be associated with symptoms characterized as bald sea urchin disease in S. ...

    • Modeling the "microbial chassis effect" on the performance of a genetic switch 

      Verusevski, Vanja (Master thesis; Mastergradsoppgave, 2022-03-21)
      Escherichia coli is one of the most established bacterial hosts for genetic devices, partially due to the available knowledge and tools for ease of manipulation. However, there is an incentive to increase the current number of available recipients. For instance, marine bacteria are being recognized for their potential as microbial "chassis" due to their rich genetic and metabolic diversity. With ...

    • Naphthoquinone pigments from the green sea urchin, Strongylocentrotus droebachiensis 

      Knutsen, Haakon (Master thesis; Mastergradsoppgave, 2021-02-08)
      Spinochromes are part of a subgroups of quinones called naphthoquinones. These molecules have a series of bioactivity making them a promising target for marine researchers all over the world. Sulphated spinochromes was recently identified, but their structure or bioactivity is yet to be elucidated. In this project, an attempt to extract these sulphated spinochromes from red spherule cells are made. ...

    • Oksidativ stabilitet til kommersielle fiskeoljeprodukter 

      Brun, Alf Thorstein (Master thesis; Mastergradsoppgave, 2012-11-15)
      Markedet for produkter med langkjedede omega-3 fettsyrer har de siste årene vært i stadig vekst. Dette kommer av godt dokumenterte positive helseeffekter spesielt ved forebygging av hjerte- og karsykdommer. Hoveddelen av disse omega-3 oljene produseres i dag av fete fiskesorter fra farvannene rundt Sør-Amerika og mesteparten (40 %) raffineres i Norge. Omega-3 produkter har gjerne et konsentrert ...

    • Påvisning av sekundærmetabolitter i marine bakterieisolater 

      Wedø, Charlotte (Master thesis; Mastergradsoppgave, 2011-06-14)
      Bioaktive forbindelser påvist hos marine invertebrater ses ofte å ha strukturelle likheter med bakterielle sekundærmetabolitter og antas derfor å være produsert av mikrobielle symbionter. Målet med oppgaven var å screene småskala kulturfiltrater fra marine bakterieisolater fra svamper og sjøpungen Synoicum pulmonaria for å identifisere mulige produsenter av sekundærmetabolitter ved sammenligning ...

    • Phototrophic, heterotrophic and mixotrophic growth in cold-adapted marine microalgae 

      Tovar Aguirre, Ana Lilia (Master thesis; Mastergradsoppgave, 2022-05-15)
      Microalgae are grown in different habitats and conditions, such as high salinity, extreme temperatures, and variable pH. Cold-adapted microalgae may have the capacity of generating high biomass production, which can potentially be produced under cold and light-limited conditions in the Arctic. Most microalgae are obligated photoautotrophs; however, few species have been found living in complete ...

    • Protein og kitin i grakse fra produksjon av Calanus® Oil 

      Udin, Therese Christina (Master thesis; Mastergradsoppgave, 2017-05-15)
      Letingen etter nye ressurser som er rik på flerumettede langkjedede omega-3 fettsyrer, har ført til utnyttelse av dyreplanktonet Calanus finmarchicus (raudåte). Det blir høstet inn ved tråling i overflaten under de mest lipidrike stadiene i vår- og sommermånedene. Den største andelen av lipidene er voksestere med et høyt innhold av omega-3 fettsyrer; stearidonsyre (18:4n-4), EPA (20:5n-3) og DHA ...

    • The Role of Silicate in the Parasitism of Pleurosigma sp. Diatoms by the Thraustochytrid Phycophthorum isakeiti 

      Powers, Daniel Jerome Finley (Master thesis; Mastergradsoppgave, 2020-05-15)
      The recently described thraustochytrid diatom parasite Phycophthorum isakeiti has been isolated from the marine system of northern Norway (Hassett, 2020). Protist P. isakeiti’s ecological role, biodiscovery potential, and interactions with a possible diatom host, Pleurosigma sp. remain largely unexplored. Here I present culturing experiments to test whether P. isakeiti is an obligate parasite. To ...

    • SAV Ag CpG/polyI:C formulated vaccination potentiate protective immune responses in Atlantic salmon - no additive or synergistic effect present when co-injecting a Rhabdovirus G-DNA vaccine 

      Thim, Hanna Leena (Master thesis; Mastergradsoppgave, 2013-02-11)
      Vaccines of today rely on adjuvant efficacy to provide a link between innate and adaptive immunity. CpG oligodeoxynucleotides (ODN) and polyinosinic:polycytidylic acid (polyI:C) are both toll-like receptor (TLR) ligands signaling through a combination of pathogen recognition receptors (PRR) and in mammals CpG/polyI:C are known to induce T-helper 1 (Th1) and cytotoxic T-lymphocyte (CTL) responses. ...

    • Securidine analogues from the marine bryozoan Securiflustra securifrons. 

      Salamonsen, Daniel (Master thesis; Mastergradsoppgave, 2021-05-12)
      Har isolert analoger av Securidine A, samt andre forbindelser fra arten Securiflustra securifrons, utført strukturoppklaring og testet for bioaktivitet.

    • Uptake of Polystyrene Nanoplastic and its Impacts on the Proteome of Salmonid cells 

      Heim, Oda (Master thesis; Mastergradsoppgave, 2023-06-27)
      Micro- and nanoplastic particles, produced from the breakdown of larger plastic debris, represent a whole new field of hazardous pollution with the potential to cause great damage on a range of marine species. This escalating concern of plastic pollution in marine environments and its consequential impacts on marine species, has emphasized the urgency for an in-depth understanding of the uptake and ...