Blar i forfatter Artikler, rapporter og annet (fysikk og teknologi) "Acuna Maldonado, Sebastian Andres"

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    • Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections 

      Villegas, Luis; Dubey, Vishesh Kumar; Nystad, Mona; Tinguely, Jean-Claude; Coucheron, David Andre; Dullo, Firehun Tsige; Priyadarshi, Anish; Acuna Maldonado, Sebastian Andres; Ahmad, Azeem; Mateos, Jose M.; Barmettler, Gery; Ziegler, Urs; Birgisdottir, Åsa Birna; Hovd, Aud-Malin Karlsson; Fenton, Kristin Andreassen; Acharya, Ganesh; Agarwal, Krishna; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-24)
      Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used in histological investigations, their resolving capabilities are insufficient to visualize details at subcellular level. Although a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases, the ...

    • Deriving high contrast fluorescence microscopy images through low contrast noisy image stacks 

      Acuna Maldonado, Sebastian Andres; ROY, MAYANK; Villegas, Luis; Dubey, Vishesh Kumar; Ahluwalia, Balpreet Singh; Agarwal, Krishna (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-11)
      Contrast in fluorescence microscopy images allows for the differentiation between different structures by their difference in intensities. However, factors such as point-spread function and noise may reduce it, affecting its interpretability. We identified that fluctuation of emitters in a stack of images can be exploited to achieve increased contrast when compared to the average and Richardson-Lucy ...

    • Scalable-resolution structured illumination microscopy 

      Butola, Ankit; Acuna Maldonado, Sebastian Andres; Hansen, Daniel Henry; Agarwal, Krishna (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-11-15)
      Structured illumination microscopy suffers from the need of sophisticated instrumentation and precise calibration. This makes structured illumination microscopes costly and skill-dependent. We present a novel approach to realize super-resolution structured illumination microscopy using an alignment non-critical illumination system and a reconstruction algorithm that does not need illumination ...