Now showing items 1-10 of 46
Characterization of a waveguide Mach-Zehnder interferometer using PDMS as a cover layer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015)
A Mach-Zehnder interferometer made with shallow rib waveguides is studied experimentally and using simulations. The rib-height giving single-mode guidance is found as function of core thickness and polarization. Devices have been made using shallow rib waveguides (5 nm rib height) in silicon nitride. The sensitivity and the limit of detection (LOD) is studied experimentally regarding the length of ...
Optical trapping and propulsion of red blood cells on waveguide surfaces
(Journal article; Tidsskriftartikkel; Peer reviewed, 2010)
New ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrations
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-01-10)
Super-resolution fluorescence microscopy, also known as nanoscopy, has provided us with a glimpse of future impacts on cell biology. Far-field optical nanoscopy allows, for the first time, the study of sub-cellular nanoscale biological structures in living cells, which in the past was limited to electron microscopy (EM) (in fixed/dehydrated) cells or tissues. Nanoscopy has particular utility in the ...
Rib waveguides for trapping and transport of particles
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-02-23)
Rib waveguides are investigated as an alternative to strip waveguides for planar trapping and transport of microparticles. Microparticles are successfully propelled along the surface of rib waveguides and trapped in the gap between opposing rib waveguides. The trapping capabilities of waveguide end facets formed by a single and opposing waveguide geometries are investigated. The slab beneath a rib ...
Inflammatory response of macrophages and trophoblasts investigated using structured illumination microscopy and quantitative phase microscopy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-09)
<i>Introduction</i>: The super-resolution capability of structured illumination microscopy (SIM) (100 nm) enables 3D imaging of mitochondria, and label-free quantitative phase microscopy (QPM) provides nanoscale quantitative phase values relating to cellular thickness and the refractive index of cellular content.
High-resolution optical nanoscopy of placental cells
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017)
<i>Introduction</i>: The invention of fluorescence based super-resolution optical microscopy, commonly referred to as optical nanoscopy, has provided a glimpse of its future impacts on life science and medical care. Optical nanoscopy enables study of sub-cellular nanoscale biological systems in living cells, which in past was limited to electron microscopy (in fixed cells). Optical nanoscopy provide ...
Optical transport, lifting and trapping of micro-particles by planar waveguides
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-03-03)
Optical waveguides can be used to trap and transport micro-particles. The particles are held close to the waveguide surface by the evanescent field and propelled forward. We propose a new technique to lift and trap particles above the surface of the waveguides. This is made possible by a gap between two opposing, planar waveguides. The field emitted from each of the waveguide ends diverge fast, away ...
Serial Raman spectroscopy of particles trapped on a waveguide
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
We demonstrate that Raman spectroscopy can be used to characterize and identify particles that are trapped and propelled along optical waveguides. To accomplish this, microscopic particles on a waveguide are moved along the waveguide and then individually addressed by a focused laser beam to obtain their characteristic Raman signature within 1 second acquisition time. The spectrum is used to distinguish ...
Quantitative phase microscopy of red blood cells during planar trapping and propulsion
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-08-22)
Red blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling and deforming large cell populations along the length of the waveguide. Bright field microscopy employed with waveguide trapping does not provide quantitative information about ...
Multi-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophages
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-24)
Total internal reflection fluorescence (TIRF) microscopy benefits from high-sensitivity, low background noise, low photo-toxicity and high-contrast imaging of sub-cellular structures close to the membrane surface. Although, TIRF microscopy provides high-contrast imaging it does not provide quantitative information about morphological features of the biological cells. Here, we propose an integrated ...