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dc.contributor.authorSvistounov, Dmitri
dc.contributor.authorWarren, Alessandra
dc.contributor.authorMcNerney, Gregory P
dc.contributor.authorOwen, Dylan M.
dc.contributor.authorZencak, Dusan
dc.contributor.authorZykova, Svetlana N
dc.contributor.authorCrane, Harry
dc.contributor.authorHuser, Thomas
dc.contributor.authorQuinn, Ronald J.
dc.contributor.authorSmedsrød, Bård
dc.contributor.authorLe Couteur, David G
dc.contributor.authorCogger, Victoria C
dc.date.accessioned2013-03-13T10:12:25Z
dc.date.available2013-03-13T10:12:25Z
dc.date.issued2012
dc.description.abstractFenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional structured illumination microscopy, scanning electron microscopy, internal reflectance fluorescence microscopy and two-photon fluorescence microscopy were used to study liver sinusoidal endothelial cells isolated from mice. There was an inverse distribution between sieve plates and membrane rafts visualized by structured illumination microscopy and the fluorescent raft stain, Bodipy FL C5 ganglioside GM1. 7-ketocholesterol and/or cytochalasin D increased both fenestrations and lipid-disordered membrane, while Triton X-100 decreased both fenestrations and lipid-disordered membrane. The effects of cytochalasin D on fenestrations were abrogated by co-administration of Triton X-100, suggesting that actin disruption increases fenestrations by its effects on membrane rafts. Vascular endothelial growth factor (VEGF) depleted lipid-ordered membrane and increased fenestrations. The results are consistent with a sieve-raft interaction, where fenestrations form in non-raft lipid-disordered regions of endothelial cells once the membrane-stabilizing effects of actin cytoskeleton and membrane rafts are diminished.en
dc.identifier.citationPLoS ONE (2012), vol. 7(9): e46134en
dc.identifier.cristinIDFRIDAID 975255
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0046134
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/4971
dc.identifier.urnURN:NBN:no-uit_munin_4702
dc.language.isoengen
dc.publisherPublic Library of Science (PLoS)en
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715en
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Physiopathology: 721en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Patofysiologi: 721en
dc.titleThe Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cellsen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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