Blar i forfatter Fakultet for naturvitenskap og teknologi "Acharya, Ganesh"

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    • Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections 

      Villegas, Luis; Dubey, Vishesh Kumar; Nystad, Mona; Tinguely, Jean-Claude; Coucheron, David Andre; Dullo, Firehun Tsige; Priyadarshi, Anish; Acuna Maldonado, Sebastian Andres; Ahmad, Azeem; Mateos, Jose M.; Barmettler, Gery; Ziegler, Urs; Birgisdottir, Åsa Birna; Hovd, Aud-Malin Karlsson; Fenton, Kristin Andreassen; Acharya, Ganesh; Agarwal, Krishna; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-24)
      Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used in histological investigations, their resolving capabilities are insufficient to visualize details at subcellular level. Although a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases, the ...

    • High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination 

      Ahmad, Azeem; Dubey, Vishesh; Jayakumar, Nikhil; Habib, Anowarul; Butola, Ankit; Nystad, Mona; Acharya, Ganesh; Basnet, Purusotam; Mehta, Dalip Singh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-04)
      High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence light sources are implemented to achieve high spatial phase sensitivity in QPM at the cost of either reduced temporal resolution or smaller field ...

    • Inflammatory response of macrophages and trophoblasts investigated using structured illumination microscopy and quantitative phase microscopy 

      Singh, Rajwinder; Wolfson, Deanna Lynn; Dubey, Vishesh; Ahmad, Azeem; Acharya, Ganesh; Mehta, Dalip Singh; Basnet, Purusotam; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-09)
      <i>Introduction</i>: The super-resolution capability of structured illumination microscopy (SIM) (100 nm) enables 3D imaging of mitochondria, and label-free quantitative phase microscopy (QPM) provides nanoscale quantitative phase values relating to cellular thickness and the refractive index of cellular content.

    • Live-cell imaging of human spermatozoa using structured illumination microscopy 

      Opstad, Ida Sundvor; Popova, Daria; Acharya, Ganesh; Basnet, Purusotam; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-05)
      Structural details of spermatozoa are interesting from the perspectives of fundamental biology and growing reproductive health problems. Studies of nanostructural details of these extremely motile cells have been limited to fixed cells, largely using electron microscopy. Here we provide the protocols for and demonstrate live-cell multi-color super-resolution imaging of human spermatozoa using ...

    • Multi-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophages 

      Dubey, Vishesh; Ahmad, Azeem; Singh, Rajwinder; Wolfson, Deanna; Basnet, Purusotam; Acharya, Ganesh; Mehta, Dalip Singh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-24)
      Total internal reflection fluorescence (TIRF) microscopy benefits from high-sensitivity, low background noise, low photo-toxicity and high-contrast imaging of sub-cellular structures close to the membrane surface. Although, TIRF microscopy provides high-contrast imaging it does not provide quantitative information about morphological features of the biological cells. Here, we propose an integrated ...

    • Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress condition 

      Dubey, Vishesh Kumar; Popova, Daria; Ahmad, Azeem; Acharya, Ganesh; Basnet, Purusotam; Mehta, Dalip Singh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-05)
      Semen quality assessed by sperm count and sperm cell characteristics such as morphology and motility, is considered to be the main determinant of men’s reproductive health. Therefore, sperm cell selection is vital in assisted reproductive technology (ART) used for the treatment of infertility. Conventional bright field optical microscopy is widely utilized for the imaging and selection of sperm cells ...

    • Quantitative assessment of morphology and sub-cellular changes in macrophages and trophoblasts during inflammation 

      Singh, Rajwinder; Dubey, Vishesh; Wolfson, Deanna; Ahmad, Azeem; Butola, Ankit; Acharya, Ganesh; Singh Mehta, Dalip; Basnet, Purusotam; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-12)
      In pregnancy during an inflammatory condition, macrophages present at the feto-maternal junction release an increased amount of nitric oxide (NO) and pro-inflammatory cytokines such as TNF-α and INF-γ, which can disturb the trophoblast functions and pregnancy outcome. Measurement of the cellular and sub-cellular morphological modifications associated with inflammatory responses are important in order ...

    • Visualizing ultrastructural details of placental tissue with super-resolution structured illumination microscopy 

      Villegas Hernandez, Luis Enrique; Nystad, Mona; Ströhl, Florian; Basnet, Purusotam; Acharya, Ganesh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-14)
      Super-resolution fluorescence microscopy is a widely employed technique in cell biology research, yet remains relatively unexplored in the field of histopathology. Here, we describe the sample preparation steps and acquisition parameters necessary to obtain fluorescent multicolor super-resolution structured illumination microscopy (SIM) images of both formalin-fixed paraffin-embedded and cryo-preserved ...