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DNA vaccines for fish: Review and perspectives on correlates of protection
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-10-24)
Recently in 2016, the European Medicines Agency (EMA) recommended granting a marketing authorization in the EU for “Clynav,” a DNA vaccine against salmon pancreas disease (salmonid alphavirus‐3). Generally, DNA vaccines induce both early and late immune responses in fish that may be protective against disease. Several transcriptomic approaches have been performed to map immunome profiles following ...
Adjuvants and immunostimulants in fish vaccines: Current knowledge and future perspectives.
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
Vaccination is the most adequate method to control infectious diseases that threaten the aquaculture industry worldwide. Unfortunately, vaccines are usually not able to confer protection on their own; especially those vaccines based on recombinant antigens or inactivated pathogens. Therefore, the use of adjuvants or immunostimulants is often necessary to increase the vaccine efficacy. Traditional ...
Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-03-30)
Reactive oxygen species (ROS) production in macrophage‐like cells is induced as an antimicrobial defence against invading pathogens. In this study, we have explored how different stimuli and metabolic inhibitors affect the level of respiratory burst in Atlantic salmon (<i>Salmo salar</i> L.) head kidney macrophage‐like cells. Cells stimulated in vitro by bacterial lipopolysaccharide (LPS) and ß‐glucan ...
A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016)
The long-term persistence and activity of a naked plasmid DNA (pGL3-35S) containing a luc gene (reporter gene) controlled by a plant 35S CaMV promoter was studied in Atlantic salmon (Salmo salar L.) after injection. Atlantic salmon (mean weight 70 grams) were injected intramuscularly with 100 μg of plasmid DNA. Blood, different tissues and organs were sampled at different time points up to day 535 ...
Th17 master transcription factors RORα and RORγ regulate the expression of IL-17C, IL-17D and IL-17F in Cynoglossus semilaevis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-11-10)
The RAR-related orphan receptors (RORs) are members of the nuclear receptor family of intracellular transcription factors. In this study, we examined the regulatory properties of RORα (CsRORα) and RORγ (CsRORγ) in tongue sole (Cynoglossus semilaevis). CsRORα and CsRORγ expression was detected in major lymphoid organs and altered to significant extents after bacterial and viral infection. CsRORα ...
Antigen dose and humoral immune response correspond with protection for inactivated infectious pancreatic necrosis virus vaccines in Atlantic salmon (Salmo salar L)
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
An enduring challenge in the vaccinology of infectious pancreatic necrosis virus (IPNV) is the lack of correlation
between neutralizing antibodies and protection against mortality. To better understand the immunological basis of
vaccine protection, an efficacy trial including Atlantic salmon (Salmo salar L.) vaccinated with a high antigen (HiAg)
or low antigen (LoAg) dose vaccine was carried out ...
Vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicida
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-12-11)
We present a study on the effect of water temperature on immunization of Atlantic lumpfish. In total, 360 fish were vaccinated with either 50 μl of an oil‐based injection vaccine (VAX), with Aeromonas salmonicida and Vibrio salmonicida antigens, or PBS. Fish were vaccinated at three different water temperatures, 5°C, 10°C and 15°C, and sorted into six groups (N = 60). Lumpfish were weighed every 3 ...
Transcription factor T-bet in Atlantic salmon: characterization and gene expression in mucosal tissues during Aeromonas salmonicida infection
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-07-06)
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 ...
Strategies and hurdles using DNA vaccines to fish
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014)
Transgene and immune gene expression following intramuscular injection of Atlantic salmon (Salmo salar L.) with DNA-releasing PLGA nano- and microparticles
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
The use of poly-(D,L-lactic-co-glycolic) acid (PLGA) particles as carriers for DNA delivery has received considerable attention in mammalian studies. DNA vaccination of fish has been shown to elicit durable transgene expression, but no reports exist on intramuscular administration of PLGA-encapsulated plasmid DNA (pDNA). We injected Atlantic salmon (Salmo salar L.) intramuscularly with a plasmid ...