The development of in vitro methodologies has contributed significantly in elucidating mechanisms of xenobiotic toxicity in aquatic organisms. The present study aimed to develop the in vitro methodology Precision-Cut Liver Slices (PCLS) and to investigate the effects of model compounds (WY-14,643 [WY] and benzo(a)pyrene [BaP]) and two mixtures of 2- and 3-4-ring polycyclic aromatic hydrocarbons ...