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Profiling Atlantic salmon B cell populations: CpG-mediated TLR-ligation enhances IgM secretion and modulates immune gene expression
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-02-23)
While TLR-activated pathways are key regulators of B cell responses in mammals, their impact on teleost B cells are scarcely addressed. Here, the potential of Atlantic salmon B cells to respond to TLR ligands was shown by demonstrating a constitutive expression of nucleic-acid sensing TLRs in magnetic sorted IgM<sup>+</sup> cells. Of the two receptors recognizing CpG in teleosts, <i>tlr9</i> was the ...
Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar).
(Journal article; Tidsskriftartikkel; Peer reviewed, 2010)
Structural and functional characterization of salmon STAT1, STAT2 and IRF9 homologs sheds light on interferon signaling in teleosts
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014)
Mammalian IRF9 and STAT2, together with STAT1, form the ISGF3 transcription factor complex, which is critical for type I interferon (IFN)-induced signaling, while IFNγ stimulation is mediated by homodimeric STAT1 protein. Teleost fish are known to possess most JAK and STAT family members, however, description of their functional activity in lower vertebrates is still scarce. In the present study we ...
Microbial danger signals control transcriptional induction of distinct MHC class I L lineage genes in Atlantic salmon
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-11)
Antigen processing and presentation by major histocompatibility complex (MHC) molecules is a cornerstone in vertebrate immunity. Like mammals, teleosts possess both classical MHC class I and multiple families of divergent MHC class I genes. However, while certain mammalian MHC class I-like molecules have proven to be integral in immune regulation against a broad array of pathogens, the biological ...
Homing of Antigen-Presenting Cells in Head Kidney and Spleen – Salmon Head Kidney Hosts Diverse APC Types
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
Lymph nodes and spleen are major organs where mammalian antigen-presenting cells (APCs) initiate and orchestrate Ag-specific immune responses. Unlike mammals, teleosts lack lymph nodes and an interesting question is whether alternative organs may serve as sites for antigen presentation in teleosts. In the current study, fluorescent ovalbumin (Ova) and CpG oligonucleotides (ODNs) injected intra-abdominally ...
Stimulation of exosome release by extracellular DNA is conserved across multiple cell types
(Journal article; Tidsskriftartikkel; Manuskript; Peer reviewed; Preprint, 2018-06-28)
Exosomes are distinguished from other types of extracellular vesicles by their small and relatively uniform size (30-100 nm) and their composition which reflects their endo-lysosomal origin. Involvement of these extracellular organelles in intercellular communication and their implication in pathological conditions has fuelled intensive research on mammalian exosomes; however, currently, very little ...
Vaccine adjuvants in fish vaccines make a difference: comparing the effects of three adjuvants (Montanide ISA763A oil, CpG/poly I:C combo and VHSV glycoprotein) used alone or in combination to formulate an inactivated salmonid alphavirus whole virus antigen
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014)
Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes
(Conference object; Konferansebidrag, 2015-06-28)
Exosomes are secreted nanosize vesicles (30−100 nm) derived from multivesicular endosomes. Exosomes are released by different immune cell types, including T- and B-lymphocytes, mast
cells and antigen-presenting cells (APCs). The composition of exosomes - including protein and RNA content reflects their endosomal origin and the type of cells that produce them.
Mammalian APCs produce large amounts ...
Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
(Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference
genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable during experimental infection with salmon ...
IPNV with high and low virulence: host immune responses and viral mutations during infection
(Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
Infectious pancreatic necrosis virus (IPNV) is an aquatic member of the Birnaviridae family that causes widespread disease in salmonids. IPNV is represented by multiple strains with markedly different virulence. Comparison of isolates reveals hyper variable regions (HVR), which are presumably associated with pathogenicity. However little is known about the rates and modes of sequence divergence and ...