• Clitellate worms (Annelida) in late-glacial and Holocene sedimentary DNA records from the Polar Urals and northern Norway 

      Lammers, Youri; Clarke, Charlotte; Erséus, Christer; Brown, Antony Gavin; Edwards, Mary Elizabeth; Gielly, Ludovic; Haflidason, Haflidi; Mangerud, Jan; Rota, E; Svendsen, John-Inge; Alsos, Inger Greve (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-26)
      While there are extensive macro‐ and microfossil records of a range of plants and animals from the Quaternary, earthworms and their close relatives amongst annelids are not preserved as fossils and therefore the knowledge of their past distributions is limited. This lack of fossils means that clitellate worms (Annelida) are currently underused in palaeoecological research, even though they can provide ...
    • High resolution ancient sedimentary DNA shows that alpine plant diversity is associated with human land use and climate change 

      Garces Pastor, Sandra; Alsos, Inger Greve; Coissac, Eric; Lavergne, Sébastien; Schwörer, Christoph; Theurillat, Jean-Paul; Heintzman, Peter D.; Wangensteen, Owen S.; Tinner, Willy; Rey, Fabian; Heer, Martina; Rutzer, Astrid; Walsh, Kevin; Lammers, Youri; Brown, Antony G.; Goslar, Tomasz; Rijal, Dilli P.; Karger, Dirk N.; Pellissier, Loïc; Heiri, Oliver (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-11-04)
      The European Alps are highly rich in species, but their future may be threatened by ongoing changes in human land use and climate. Here, we reconstructed vegetation, temperature, human impact and livestock over the past ~12,000 years from Lake Sulsseewli, based on sedimentary ancient plant and mammal DNA, pollen, spores, chironomids, and microcharcoal. We assembled a highly-complete local DNA ...
    • High-throughput sequencing of African chikanda cake highlights conservation challenges in orchids 

      Veldman, Sarina; Gravendeel, Barbara; Otieno, Joseph; Lammers, Youri; Duijm, Elza; Nieman, Aline; Bytebier, Benny; Ngugi, Grace; Martos, Florent; van Andel, TR; de Boer, Hugo (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-04-01)
      Chikanda is a traditional dish made with wild-harvested ground orchid tubers belonging to three orchidioid genera, Disa, Satyrium and Habenaria, all of which are CITES appendix II-listed. Identification of collected orchid tubers is very difficult and documentation of constituent species in prepared chikanda has hitherto been impossible. Here amplicon metabarcoding was used in samples of six prepared ...
    • Holocene floristic diversity and richness in northeast Norway revealed by sedimentary ancient DNA (sedaDNA) and pollen 

      Clarke, Charlotte; Edwards, Mary Elizabeth; Brown, Antony Gavin; Gielly, Ludovic; Lammers, Youri; Heintzman, Peter D.; Murguzur, Francisco Javier Ancin; Bråthen, Kari Anne; Goslar, Tomasz; Alsos, Inger Greve (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-30)
      We present a Holocene record of floristic diversity and environmental change for the central Varanger Peninsula, Finnmark, based on ancient DNA extracted from the sediments of a small lake (<i>sed</i>aDNA). The record covers the period c. 10 700 to 3300 cal. a BP and is complemented by pollen data. Measures of species richness, sample evenness and beta diversity were calculated based on <i>sed</i>aDNA ...
    • Paleoeconomy more than demography determined prehistoric human impact in Arctic Norway 

      Brown, Antony; Rijal, Dilli Prasad; Heintzman, Peter D.; Clarke, Charlotte, L.; Blankholm, Hans Peter; Høeg, Helge I.; Lammers, Youri; Bråthen, Kari Anne; Edwards, M E; Alsos, Inger Greve (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-10-07)
      Population size has increasingly been taken as the driver of past human environmental impact worldwide, and particularly in the Arctic. However, sedimentary ancient DNA (sedaDNA), pollen and archaeological data show that over the last 12,000 years, paleoeconomy and culture determined human impacts on the terrestrial ecology of Arctic Norway. The large Mortensnes site complex (Ceavccagea ¯dgi, 70◦N) ...
    • Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation 

      Alsos, Inger Greve; Lammers, Youri; Yoccoz, Nigel Gilles; Jørgensen, Tina; Sjögren, Per; Gielly, Ludovic; Edwards, Mary E. (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-04-17)
      Metabarcoding of lake sediments have been shown to reveal current and past biodiversity, but little is known about the degree to which taxa growing in the vegetation are represented in environmental DNA (eDNA) records. We analysed composition of lake and catchment vegetation and vascular plant eDNA at 11 lakes in northern Norway. Out of 489 records of taxa growing within 2 m from the lake shore, ...
    • Pollen, macrofossils and sedaDNA reveal climate and land use impacts on Holocene mountain vegetation of the Lepontine Alps, Italy 

      van Vugt, Lieveke; Garces Pastor, Sandra; Gobet, Erika; Brechbühl, Sarah; Knetge, Antonietta; Lammers, Youri; Stengele, Katja; Alsos, Inger Greve; Tinner, Willy; Schwörer, Christoph (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-10-10)
      Both climate change and anthropogenic disturbance affect vegetation composition, but it is difficult to separate these drivers of vegetation change from one another. A better understanding of past vegetation dynamics is necessary to disentangle the influence of different forcing factors and assess future vegetation change. Here we present the first multi-proxy palaeoecological study combining ...
    • Postglacial species arrival and diversity buildup of northern ecosystems took millennia 

      Alsos, Inger Greve; Rijal, Dilli Prasad; Ehrich, Dorothee; Karger, Dirk Nikolaus; Yoccoz, Nigel; Heintzman, Peter D.; Brown, Antony; Lammers, Youri; Pellissier, Loïc; Alm, Torbjørn; Bråthen, Kari Anne; Coissac, Eric; Merkel, Marie Føreid; Alberti, Adriana; Denoeud, France; Bakke, Jostein (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-09-28)
      What drives ecosystem buildup, diversity, and stability? We assess species arrival and ecosystem changes across 16 millennia by combining regional-scale plant sedimentary ancient DNA from Fennoscandia with near-complete DNA and trait databases. We show that postglacial arrival time varies within and between plant growth forms. Further, arrival times were mainly predicted by adaptation to temperature, ...
    • The sedimentary ancient DNA workflow 

      Heintzman, Peter D.; Nota, Kevin; Rouillard, Alexandra; Lammers, Youri; Murchie, Tyler; Armbrecht, Linda; Garcés-Pastor, Sandra; Vernot, Benjamin (Chapter; Bokkapittel, 2023)
      Sedimentary ancient DNA (sedaDNA) is continuing to revolutionise our understanding of past biological and geological processes by retrieving and analysing the ancient DNA preserved in lake, cave, open terrestrial, midden, permafrozen, and marine environments (Crump, 2021). The study of sedaDNA began in the late 1990s (Coolen and Overmann, 1998) with the first reports of extinct animal sedaDNA in ...
    • Shotgun-sequencing of plant DNA: Selection og material and methods 

      Merkel, Marie Kristine Føreid; Pitelkova, Iva; Lammers, Youri; Coissac, Eric; Alberti, Adriana; Dossat, Carole; Kirchefer, Andreas; Alsos, Inger Greve (Conference object; Konferansebidrag, 2017)
      Initially, rbcLaand ITS2 were PCR amplified and Sanger sequenced for 564 species from 1900 specimens. To increase the amount of the genome covered, shotgun sequencing of 1670 species is in progress. Our Sanger analyses had a success rate of 74% and 85% for ITS2 and rbcLa, respectively. Overall success rate of Sanger sequencing decreased with later collection date in the year. Preliminary results of ...