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    • Minimizing polymerase biases in metabarcoding 

      Nichols, Ruth V.; Vollmers, Christopher; Newsom, Lee A.; Wang, Yue; Heintzman, Peter D.; Leighton, McKenna; Green, Richard E.; Shapiro, Beth (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-05-24)
      DNA metabarcoding is an increasingly popular method to characterize and quantify biodiversity in environmental samples. Metabarcoding approaches simultaneously amplify a short, variable genomic region, or “barcode,” from a broad taxonomic group via the polymerase chain reaction (PCR), using universal primers that anneal to flanking conserved regions. Results of these experiments are reported as ...