• High-throughput sequencing of African chikanda cake highlights conservation challenges in orchids 

      Veldman, Sarina; Gravendeel, Barbara; Otieno, Joseph; Lammers, Youri; Duijm, Elza; Nieman, Aline; Bytebier, Benny; Ngugi, Grace; Martos, Florent; van Andel, TR; de Boer, Hugo (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-04-01)
      Chikanda is a traditional dish made with wild-harvested ground orchid tubers belonging to three orchidioid genera, Disa, Satyrium and Habenaria, all of which are CITES appendix II-listed. Identification of collected orchid tubers is very difficult and documentation of constituent species in prepared chikanda has hitherto been impossible. Here amplicon metabarcoding was used in samples of six prepared ...
    • Plant biodiversity assessment through soil eDNA reflects temporal and local diversity 

      Ariza Salazar, Maria; Fouks, Bertrand; Mauvisseau, Quentin; Halvorsen, Rune; Alsos, Inger Greve; de Boer, Hugo (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-04-12)
      1. Several studies have shown the potential of eDNA-based proxies for plant identification, but little is known about their spatial and temporal resolution. This limits its use for plant biodiversity assessments and monitoring of vegetation responses to environmental changes. Here we calibrate the temporal and spatial plant signals detected with soil eDNA surveys by comparing with a standard visual ...