dc.contributor.author | Samuelsen, Ørjan | |
dc.contributor.author | Overballe-Petersen, Søren | |
dc.contributor.author | Bjørnholt, Jørgen | |
dc.contributor.author | Brisse, Sylvain | |
dc.contributor.author | Doumith, Michel | |
dc.contributor.author | Woodford, Neil | |
dc.contributor.author | Hopkins, Katie L. | |
dc.contributor.author | Aasnæs, Bettina | |
dc.contributor.author | Haldorsen, Bjørg | |
dc.contributor.author | Sundsfjord, Arnfinn | |
dc.date.accessioned | 2018-02-14T08:23:56Z | |
dc.date.available | 2018-02-14T08:23:56Z | |
dc.date.issued | 2017-11-15 | |
dc.description.abstract | The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing worldwide. Here we present associated patient data and molecular, epidemiological and phenotypic characteristics of all CPE isolates in Norway from 2007 to 2014 confirmed at the Norwegian National Advisory Unit on Detection of Antimicrobial Resistance. All confirmed CPE isolates were characterized pheno- and genotypically, including by whole genome sequencing (WGS). Patient data were reviewed retrospectively. In total 59 CPE isolates were identified from 53 patients. Urine was the dominant clinical sample source (37%) and only 15% of the isolates were obtained from faecal screening. The majority of cases (62%) were directly associated with travel or hospitalization abroad, but both intra-hospital transmission and one inter-hospital outbreak were observed. The number of CPE cases/year was low (2–14 cases/year), but an increasing trend was observed. Klebsiella spp. (n = 38) and E. coli (n = 14) were the dominant species and bla<sub>KPC</sub> (n = 20), bla<sub>NDM</sub> (n = 19), bla<sub>OXA-48-like</sub> (n = 12) and bla<sub>VIM</sub> (n = 7) were the dominant carbapenemase gene families. The CPE isolates were genetically diverse except for K. pneumoniae where clonal group 258 associated with bla<sub>KPC</sub> dominated. All isolates were multidrug-resistant and a significant proportion (21%) were resistant to colistin. Interestingly, all bla<sub>OXA-48-like</sub>, and a large proportion of bla<sub>NDM</sub>-positive Klebsiella spp. (89%) and E. coli (83%) isolates were susceptible in vitro to mecillinam. Thus, mecillinam could have a role in the treatment of uncomplicated urinary tract infections caused by OXA-48- or NDM-producing E. coli or K. pneumoniae. In conclusion, the impact of CPE in Norway is still limited and mainly associated with travel abroad, reflected in the diversity of clones and carbapenemase genes. | en_US |
dc.description | Source at <a href=https://doi.org/10.1371/journal.pone.0187832> https://doi.org/10.1371/journal.pone.0187832 </a> | en_US |
dc.identifier.citation | Samuelsen, Ø., Overballe-Petersen, S., Bjørnholt, J., Brisse, S., Doumith, M., Woodford, N. & Sundsfjord A. (2017). Molecular and epidemiological characterization of carbapenemase-producing Enterobacteriaceae in Norway, 2007 to 2014. PLoS ONE. 12(11). | en_US |
dc.identifier.cristinID | FRIDAID 1530335 | |
dc.identifier.doi | 10.1371/journal.pone.0187832 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/10037/12155 | |
dc.language.iso | eng | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.journal | PLoS ONE | |
dc.rights.accessRights | openAccess | en_US |
dc.subject | VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715 | en_US |
dc.subject | VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715 | en_US |
dc.title | Molecular and epidemiological characterization of carbapenemase-producing Enterobacteriaceae in Norway, 2007 to 2014 | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |