Show simple item record

dc.contributor.authorKolton, Cari B
dc.contributor.authorPodnecky, Nicole L.
dc.contributor.authorShadomy, Sean V
dc.contributor.authorGee, Jay E
dc.contributor.authorHoffmaster, Alex R
dc.date.accessioned2018-02-23T11:54:34Z
dc.date.available2018-02-23T11:54:34Z
dc.date.issued2017-11-16
dc.description.abstractBackground: <br> Bacillus anthracis, which causes anthrax in humans and animals, is enzootic in parts of the U.S. state of Texas where cases are typically reported in animals annually. The gamma phage lysis assay is a common diagnostic method for identifcation of B. anthracis and is based on the bacterium’s susceptibility to lysis. This test has been shown to be 97% specifc for B. anthracis, as a small number of strains of other Bacillus spp. are known to be susceptible. In this study, we evaluated the performance of a combination of B. anthracis diagnostic assays on 700 aerobic, spore-forming isolates recovered from soil collected in Texas. These assays include phenotypic descriptions, gamma phage susceptibility, and real-time polymerase chain reaction specifc for B. anthracis. Gamma phage-susceptible isolates were also tested using cell wall and capsule direct fuorescent-antibody assays specifc for B. anthracis. Gamma phage-susceptible isolates that were ruled out as B. anthracis were identifed by 16S rRNA gene sequencing. <br> Findings: <br> We identifed 29 gamma phage-susceptible isolates. One was confrmed as B. anthracis, while the other 28 isolates were ruled out for B. anthracis by the other diagnostic tests. Using 16S rRNA gene sequencing results, we identifed these isolates as members of the B. cereus group, Bacillus sp. (not within B. cereus group), Lysinibacillus spp., and Solibacillus silvestris. Based on these results, we report a specifcity of 96% for gamma phage lysis as a diagnostic test for B. anthracis, and identifed susceptible isolates outside the Bacillus genus. <br> Conclusions: <br> In this study we found gamma phage susceptibility to be consistent with previously reported results. However, we identifed non-B. anthracis environmental isolates (including isolates from genera other than Bacillus) that are susceptible to gamma phage lysis. To date, susceptibility to gamma phage lysis has not been reported in genera other than Bacillus. Though these isolates are not of clinical origin, description of unexpected positives is important, especially as new diagnostic assays for B. anthracis are being developed based on gamma phage lysis or gamma phage proteins.en_US
dc.descriptionSource at: <a href=https://doi.org/10.1186/s13104-017-2919-8> https://doi.org/10.1186/s13104-017-2919-8 </a>en_US
dc.identifier.citationKolton, C. B., Podnecky, N. L., Shadomy, S. V., Gee, J. E. & Hoffmaster, A. R. (2017). Bacillus anthracis gamma phage lysis among soil bacteria: An update on test specificity. BMC Research Notes, 10(1), 2-6. https://doi.org/10.1186/s13104-017-2919-8en_US
dc.identifier.cristinIDFRIDAID 1542917
dc.identifier.doi10.1186/s13104-017-2919-8
dc.identifier.issn1756-0500
dc.identifier.urihttps://hdl.handle.net/10037/12207
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.relation.journalBMC Research Notes
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776en_US
dc.titleBacillus anthracis gamma phage lysis among soil bacteria: An update on test specificityen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record