• Are well-studied marine biodiversity hotspots still blackspots for animal barcoding? 

      Mugnai, Francesco; Meglécz, Emese; Costantini, Federica; Abbiati, Marco; Bavestrello, Giorgio; Bertasi, Fabio; Bo, Marzia; Capa, Maria; Chenuil, Anne; Colangelo, Marina Antonia; De Clerck, Olivier; Gutiérrez, José Miguel; Lattanzi, Loretta; Leduc, Michèle; Martin, Daniel; Matterson, Kenan Oguz; Mikac, Barbara; Plaisance, Laetitia; Ponti, Massimo; Riesgo, Ana; Turicchia, Eva; Waeschenbach, Andrea; Wangensteen, Owen S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-10)
      Marine biodiversity underpins ecosystem health and societal well-being. Preservation of biodiversity hotspots is a global challenge. Molecular tools, like DNA barcoding and metabarcoding, hold great potential for biodiversity monitoring, possibly outperforming more traditional taxonomic methods. However, metabarcoding-based biodiversity assessments are limited by the availability of sequences in ...
    • Bat echolocation plasticity in allopatry: a call for caution in acoustic identification of Pipistrellus sp. 

      Montauban, Cecilia; Mas, Maria; Tuneu-Corral, Carme; Wangensteen, Owen S.; Budinski, Ivana; Martí-Carreras, Joan; Flaquer, Carles; Puig-Montserrat, Xavier; López-Baucells, Adrià (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-23)
      Animals modify their behaviours and interactions in response to changing environments. In bats, environmental adaptations are reflected in echolocation signalling that is used for navigation, foraging and communication. However, the extent and drivers of echolocation plasticity are not fully understood, hindering our identification of bat species with ultrasonic detectors, particularly for cryptic ...
    • Bats as natural samplers: First record of the invasive pest rice water weevil Lissorhoptrus oryzophilus in the Iberian Peninsula 

      Montauban, Cecilia; Mas, Maria; Wangensteen, Owen S.; Sarto i Monteys, Víctor; Gisbert Fornós, David; Ferré Mola, Xavi; López-Baucells, Adrià (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-19)
      We face an increasing global food security challenge as the human population continues to grow across the globe. As agricultural production rises to keep up with food demand, so too does the expansion of crop detrimental pest species. Early detection can be crucial to control their damage and relies on the use of accurate and dependable techniques. We report the first record of rice water weevil ...
    • DNA metabarcoding reveals the importance of gelatinous zooplankton in the diet of Pandalus borealis, a keystone species in the Arctic 

      Urban, Paulina; Præbel, Kim; Bhat, Shripathi; Dierking, Jan; Wangensteen, Owen S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-22)
      Information about the dietary composition of a species is crucial to understanding their position and role in the food web. Increasingly, molecular approaches such as DNA metabarcoding are used in studying trophic relationships, not least because they may alleviate problems such as low taxonomic resolution or underestimation of digestible taxa in the diet. Here, we used DNA metabarcoding with universal ...
    • DNA metabarcoding unveils niche overlapping and competition among Caribbean sea urchins 

      Rodríguez-Barreras, Ruber; Godoy-Vitorino, Filipa; Præbel, Kim; Wangensteen, Owen S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-10)
      Detailed information of trophic interactions among consumer–resources in food webs is usually limited due to the lack of accurate identification of eaten food resources. The use of DNA-metabarcoding has been proven useful for molecular identification of the numerous taxa present in stomach contents. Here, we characterize the diet and trophic behavior of four sea urchin species inhabiting shallow ...
    • Estuarine molecular bycatch as a landscape-wide biomonitoring tool 

      Mariani, Stefano; Harper, Linsey R.; Collins, Rupert A.; Baillie, Charles; Wangensteen, Owen S.; McDevitt, Allan D.; Heddell-Cowie, Morton; Genner, Martin J. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-13)
      Environmental DNA analysis is rapidly transforming biodiversity monitoring and bolstering conservation applications worldwide. This approach has been assisted by the development of metabarcoding PCR primers that are suited for detection of a wide range of taxa. However, little effort has gone into exploring the value of the non-target DNA sequences that are generated in every survey, but subsequently ...
    • Fine-scale differences in eukaryotic communities inside and outside salmon aquaculture cages revealed by eDNA metabarcoding 

      Turon, Marta; Nygaard, Markus; Guri, Gledis; Wangensteen, Owen S.; Præbel, Kim (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-08-26)
      Aquaculture impacts on marine benthic ecosystems are widely recognized and monitored. However, little is known about the community changes occurring in the water masses surrounding aquaculture sites. In the present study, we studied the eukaryotic communities inside and outside salmonid aquaculture cages through time to assess the community changes in the neighbouring waters of the farm. Water ...
    • For all audiences: Incorporating immature stages into standardised spider inventories has a major impact on the assessment of biodiversity patterns 

      Domènech, Marc; Wangensteen, Owen S.; Enguídanos, Alba; Malumbres-Olarte, Jagoba; Arnedo, Miquel A. (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-04-25)
      Although arthropods are the largest component of animal diversity, they are traditionally underrepresented in biological inventories and monitoring programmes. However, no biodiversity assessment can be considered informative without including them. Arthropod immature stages are often discarded during sorting, despite frequently representing more than half of the collected individuals. To date, ...
    • Metabarcoding reveals high-resolution biogeographical and metaphylogeographical patterns through marine barriers 

      Antich, Adrià; Palacín, Creu; Zarcero, Jesús; Wangensteen, Owen S.; Turon, Xavier (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-12-29)
      Aim: It has been predicted that there should be concordance between biogeographical and phylogeographical processes structuring multi-species regional assemblages. We hypothesise that oceanographic barriers in the marine environment affect concomitantly the distribution and the connectivity of the marine biota, thus producing congruent biogeographical and phylogeographical structures. We also ...
    • Mitochondrial Cytochrome Oxidase Subunit 1: A Promising Molecular Marker for Species Identification in Foraminifera 

      Girard, Elsa B.; Langerak, Anouk; Jompa, Jamaluddin; Wangensteen, Owen S.; Macher, Jan-Niklas; Renema, Willem (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-02)
      Traditional morphological methods for species identification are highly time consuming, especially for small organisms, such as Foraminifera, a group of shell-building microbial eukaryotes. To analyze large amounts of samples more efficiently, species identification methods have extended to molecular tools in the last few decades. Although a wide range of phyla have good markers available, for ...
    • Monitoring bacterial community dynamics in a drinking water treatment plant: an integrative approach using metabarcoding and microbial indicators in large water volumes 

      Pinar-Méndez, Anna; Wangensteen, Owen S.; Præbel, Kim; Galofré, Belén; Méndez, Javier; Blanch, Anicet R.; García-Aljaro, Cristina (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-04-30)
      Monitoring bacterial communities in a drinking water treatment plant (DWTP) may help to understand their regular operations. Bacterial community dynamics in an advanced full-scale DWTP were analyzed by 16S rRNA metabarcoding, and microbial water quality indicators were determined at nine different stages of potabilization: river water and groundwater intake, decantation, sand filtration, ozonization, ...
    • More Than Expected From Old Sponge Samples: A Natural Sampler DNA Metabarcoding Assessment of Marine Fish Diversity in Nha Trang Bay (Vietnam) 

      Turon, Marta; Angulo-Preckler, Carlos; Antich, Adrià; Præbel, Kim; Wangensteen, Owen S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-09)
      Sponges have recently been proposed as ideal candidates to act as natural samplers for environmental DNA due to their efficiency in filtering water. However, validation of the usefulness of DNA recovered from sponges to reveal vertebrate biodiversity patterns in Marine Protected Areas is still needed. Additionally, nothing is known about how different sponge species and morphologies influence the ...
    • Niche separation between two dominant crustacean predators in European estuarine soft-bottom habitats 

      Siegenthaler, Andjin; Wangensteen, Owen S.; Benvenuto, Chiara; Lollobrigidi, Riccardo; Mariani, Stefano (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-04-05)
      Epibenthic predators in estuarine shallow soft-bottom environments are generally considered to have broad ecological niches with a wide overlap. This allows them to cope with abundant but highly variable prey communities. The assessment of trophic relationships in shallow soft-bottom habitats is, however, challenging and often complicated by the bias and low resolution of the analytical tools available ...
    • Resource competition drives an invasion-replacement event among shrew species on an island 

      Browett, Samuel S.; Synnott, Rebecca; O'Meara, Denise B.; Antwis, Rachael E.; Browett, Stephen S.; Bown, Kevin J.; Wangensteen, Owen S.; Dawson, Deborah A.; Searle, Jeremy B.; Yearsley, Jon M.; McDevitt, Allan D. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-01-08)
      Invasive mammals are responsible for the majority of native species extinctions on islands. While most of these extinction events will be due to novel interactions between species (e.g. exotic predators and naive prey), it is more unusual to find incidences where a newly invasive species causes the decline/extinction of a native species on an island when they normally coexist elsewhere in their ...
    • Spatio-temporal patterns of eukaryotic biodiversity in shallow hard-bottom communities from the West Antarctic Peninsula revealed by DNA metabarcoding 

      Preckler, Carlos Angulo; Turon, Marta; Præbel, Kim; Avila, Conxita; Wangensteen, Owen S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-05-05)
      Aim: We studied molecular eukaryotic biodiversity patterns in shallow hard-bottom Antarctic benthic communities using community DNA metabarcoding. Polar ecosystems are extremely exposed to climate change, and benthic macroinvertebrate communities have demonstrated rapid response to a range of natural and anthropogenic pressures. However, these rich and diverse ecosystems are poorly studied, ...
    • To denoise or to cluster, that is not the question: optimizing pipelines for COI metabarcoding and metaphylogeography 

      Antich, Adrià; Palacin, Creu; Wangensteen, Owen S.; Turon, Xavier (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-05)
      <i>Background</i> - The recent blooming of metabarcoding applications to biodiversity studies comes with some relevant methodological debates. One such issue concerns the treatment of reads by denoising or by clustering methods, which have been wrongly presented as alternatives. It has also been suggested that denoised sequence variants should replace clusters as the basic unit of metabarcoding ...
    • The Two Sides of the Mediterranean: Population Genomics of the Black Sea Urchin Arbacia lixula (Linnaeus, 1758) in a Warming Sea 

      Carreras, Carlos; Ordóñez, Victor; García-Cisneros, Alex; Wangensteen, Owen S.; Palacín, Creu; Pascual, Marta; Turon, Xavier (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-10)
      Global environmental changes may have a profound impact on ecosystems. In this context, it is crucial to gather biological and ecological information of the main species in marine communities to predict and mitigate potential effects of shifts in their distribution, abundance, and interactions. Using genotyping by sequencing (GBS), we assessed the genetic structure of a keystone species in the ...
    • Using DNA Metabarcoding to Characterize the Prey Spectrum of Two Co-Occurring Themisto Amphipods in the Rapidly Changing Atlantic-Arctic Gateway Fram Strait 

      Dischereit, Annkathrin; Wangensteen, Owen S.; Præbel, Kim; Auel, Holger; Havermans, Charlotte (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-11-04)
      The two congeneric hyperiids Themisto libellula and T. abyssorum provide an important trophic link between lower and higher trophic levels in the rapidly changing Arctic marine ecosystem. These amphipods are characterized by distinct hydrographic affinities and are hence anticipated to be impacted differently by environmental changes, with major consequences for the Arctic food web. In this ...