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dc.contributor.authorPetersen, Karina
dc.contributor.authorHultman, Maria Thérése
dc.contributor.authorBytingsvik, Jenny
dc.contributor.authorHarju, Mikael
dc.contributor.authorEvenset, Anita
dc.contributor.authorTollefsen, Knut Erik
dc.date.accessioned2018-04-05T08:19:55Z
dc.date.available2018-04-05T08:19:55Z
dc.date.issued2017-09-01
dc.description.abstractContaminants from various anthropogenic activities are detected in the Arctic due to long-range atmospheric transport, ocean currents, and living organisms such as migrating fish or seabirds. Although levels of persistent organic pollutants (POPs) in Arctic fish are generally low, local hot spots of contamination were found in freshwater systems such as Lake Ellasjøen at Bjørnøya (Bear Island, Norway). Higher concentrations of organic halogenated compounds (OHC), and higher levels of cytochrome P450 and DNA-double strand breaks were reported in Arctic char (Salvelinus alpinus) from this lake compared to fish from other lakes on Bjørnøya. Although several of the measured contaminants are potential endocrine disrupters, few studies have investigated potential endocrine disruptive effects of the contaminant cocktail in this fish population. The aim of this study was to compare acutely toxic and estrogenic potency of the cocktail of pollutants as evidenced by cytotoxic and/or estrogenic effects in vitro using extracts of Arctic char livers from contaminated Lake Ellasjøen with those from less contaminated Lake Laksvatn at Bjørnøya. This was performed by in situ sampling and contaminant extraction from liver tissue, followed by chemical analysis and in vitro testing of the following contaminated tissue extracts: F1-nonpolar OHC, F2-polar pesticides and metabolites of OHC, and F3-polar OHC. Contaminant levels were highest in extracts from Ellasjøen fish. The F2 and F3 extracts from Lake Laksvatn and Lake Ellasjøen fish reduced in vitro cell viability at a concentration ratio of 0.03–1 relative to tissue concentration in Arctic char. Only the F3 liver extract from Ellasjøen fish increased in vitro vitellogenin protein expression. Although compounds such as estrogenic OH-PCBs were quantified in Ellasjøen F3 extracts, it remains to be determined which compounds were inducing estrogenic effects.en_US
dc.descriptionThis is an Accepted Manuscript of an article published by Taylor & Francis Group in Journal of Toxicology and Environmental Health, Part A on 1/9/2017, available online: <a href=https://doi.org/10.1080/15287394.2017.1357277> https://doi.org/10.1080/15287394.2017.1357277 </a>.en_US
dc.identifier.citationPetersen, K., Hultman, M. T., Bytingsvik, J., Harju, M., Evenset, A., Tollefsen, K. E. (2017). Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes. Journal of Toxicology and Environmental Health. 80(16-18):1017-1030en_US
dc.identifier.cristinIDFRIDAID 1515112
dc.identifier.doi10.1080/15287394.2017.1357277
dc.identifier.issn1528-7394
dc.identifier.issn1087-2620
dc.identifier.urihttps://hdl.handle.net/10037/12472
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.relation.journalJournal of Toxicology and Environmental Health
dc.relation.projectIDNorges forskningsråd: 221371en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/MILJØ2015/196318/NORWAY/FORURENS: Non-animal (alternative) testing methods for REACH//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/MILJØFORSK/196318/NORWAY/FORURENS: Non-animal (alternative) testing methods for REACH//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/MILJØ2015/221373/NORWAY/Forurens: Is the cocktail effect of environmental contaminants a threat for Arctic fish populations?//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Toksikologi: 730en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Toxicology: 730en_US
dc.titleCharacterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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