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dc.contributor.authorWushur, Imin
dc.contributor.authorSylte, Ingebrigt
dc.contributor.authorWinberg, Jan-Olof
dc.date.accessioned2018-10-10T12:31:56Z
dc.date.available2018-10-10T12:31:56Z
dc.date.issued2014-12-24
dc.description.abstractThe present review describes the current knowledge about the reaction mechanism of drosophilid alcohol dehydrogenases (DADH), a member of the short chain dehydrogenase/reductase (SDR) superfamily. Included is the binding order of the substrates to the enzyme, rate limiting steps, stereochemistry of the reaction, active site topology, role of important amino acids and water molecules in the reaction and pH dependence of kinetic coefficients. We focus on the contribution from steady state kinetics where alternative substrates, dead end and product inhibitors, isotopes and mutated DADHs have been used as well as on the contributions from X-ray crystallography, NMR and theoretical calculations. Furthermore, we also raise some open questions in order to fully understand the reaction mechanism of this enzyme.en_US
dc.descriptionSource at <a href= https://doi.org/10.1016/j.pisc.2014.12.008> https://doi.org/10.1016/j.pisc.2014.12.008 </a>.en_US
dc.identifier.citationWushur, I., Sylte, I. & Winberg, J-O. (2015). The catalytic reaction mechanism of drosophilid alcohol dehydrogenases. Perspectives in Science, 4, 46-56. https://doi.org/10.1016/j.pisc.2014.12.008.en_US
dc.identifier.cristinIDFRIDAID 1617639
dc.identifier.doi10.1016/j.pisc.2014.12.008
dc.identifier.issn2213-0209
dc.identifier.urihttps://hdl.handle.net/10037/13936
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalPerspectives in Science
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical biochemistry: 726en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk biokjemi: 726en_US
dc.subjectDrosophilid alcohol dehydrogenasesen_US
dc.subjectTopology of binding sitesen_US
dc.subjectSteady-state kineticsen_US
dc.subjectProton transferen_US
dc.subjectShort-chain dehydrogenase/reductase superfamily (SDR)en_US
dc.titleThe catalytic reaction mechanism of drosophilid alcohol dehydrogenasesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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