Vis enkel innførsel

dc.contributor.authorLunde, Tracy Munthali
dc.contributor.authorAl-Haroni, Mohammed
dc.contributor.authorRoberts, Adam P.
dc.date.accessioned2019-01-29T17:48:34Z
dc.date.available2019-01-29T17:48:34Z
dc.date.issued2018-12-06
dc.description.abstract<p><I>Background</I>: Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these elements.</p> <p><I>Objectives and Design</I>: In this study, the CN and CR of Tn916-Tn1545 elements in oral streptococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the influence of tetracycline on the CR of Tn916-Tn1545 elements.</p> <p><I>Results</I>: The ddPCR assay designed in this study is a reliable way to rapidly determine CN and CR of Tn916-Tn1545 elements.</p> <p><I>Conclusions</i>: Our data also suggest that Tn916-Tn1545 elements are generally stable without selective pressure in the clinical oral Streptococcus strains investigated in this study.</p>en_US
dc.identifier.citationLunde, T.M., Al-Haroni, M. & Roberts, A.P. (2018). Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR. <I>Journal of Oral Microbiology, 11</I>(1). https://doi.org/10.1080/20002297.2018.1552060en_US
dc.identifier.cristinIDFRIDAID 1666072
dc.identifier.doi10.1080/20002297.2018.1552060
dc.identifier.issn2000-2297
dc.identifier.urihttps://hdl.handle.net/10037/14554
dc.language.isoengen_US
dc.publisherTaylor & Francis Openen_US
dc.relation.ispartofLunde, T.M. (2022). Antibiotic Resistance in Oral Streptococci: The prevalence, diversity, stability, and fitness cost of Tn916 -Tn1545 family in oral streptococcal isolates. (Doctoral thesis). <a href=https://hdl.handle.net/10037/24192>https://hdl.handle.net/10037/24192</a>.
dc.relation.journalJournal of Oral Microbiology
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Clinical dentistry disciplines: 830en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk odontologiske fag: 830en_US
dc.subjectOral streptococcien_US
dc.subjectTn916-Tn1545 familyen_US
dc.subjectantibiotic resistanceen_US
dc.subjectmobile genetic elements (MGEs)en_US
dc.subjectdroplet digital PCRen_US
dc.titleDetermination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCRen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel