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dc.contributor.authorOpstad, Ida Sundvor
dc.contributor.authorPopova, Daria
dc.contributor.authorAcharya, Ganesh
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2019-03-20T13:21:44Z
dc.date.available2019-03-20T13:21:44Z
dc.date.issued2018-11-05
dc.description.abstractStructural details of spermatozoa are interesting from the perspectives of fundamental biology and growing reproductive health problems. Studies of nanostructural details of these extremely motile cells have been limited to fixed cells, largely using electron microscopy. Here we provide the protocols for and demonstrate live-cell multi-color super-resolution imaging of human spermatozoa using structured illumination microscopy (SIM). By using patches of agarose for immobilization, we achieved four-channel 3D SIM imaging of the plasma membrane, nucleus, mitochondria and microtubulin in the same living sperm cells. We expect that high-resolution imaging of living spermatozoa will be implemented for research on fundamental cellular mechanisms together with morphological aberrations involved in male infertility for a future improved cell selection process in in vitro fertilization treatments.en_US
dc.description.sponsorshipUiT The Arctic Univeristy of Norwayen_US
dc.descriptionSource at <a href=https://doi.org/10.1364/BOE.9.005939> https://doi.org/10.1364/BOE.9.005939</a>. © 2018 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.en_US
dc.identifier.citationOpstad, I.S., Popova, D.A., Acharya, G., Basnet, P. & Ahluwalia, B.S. (2018). Live-cell imaging of human spermatozoa using structured illumination microscopy. <i>Biomedical Optics Express, 9</i>(12), 5939-5945. https://doi.org/10.1364/BOE.9.005939en_US
dc.identifier.cristinIDFRIDAID 1659173
dc.identifier.doi10.1364/BOE.9.005939
dc.identifier.issn2156-7085
dc.identifier.urihttps://hdl.handle.net/10037/15036
dc.language.isoengen_US
dc.publisherOptical Society of Americaen_US
dc.relation.ispartofOpstad, I.S. (2021). Bringing optical nanoscopy to life - Super-resolution microscopy of living cells. (Doctoral thesis). <a href=https://hdl.handle.net/10037/20306>https://hdl.handle.net/10037/20306</a>
dc.relation.ispartofPopova, D. (2021). Advanced methods in reproductive medicine: Application of optical nanoscopy, artificial intelligence-assisted quantitative phase microscopy and mitochondrial DNA copy numbers to assess human sperm cells. (Doctoral thesis). <a href=https://hdl.handle.net/10037/22598>https://hdl.handle.net/10037/22598</a>.
dc.relation.journalBiomedical Optics Express
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Physics: 430en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Fysikk: 430en_US
dc.titleLive-cell imaging of human spermatozoa using structured illumination microscopyen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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