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dc.contributor.authorHarper, Lynsey R.
dc.contributor.authorBuxton, Andrew S.
dc.contributor.authorRees, Helen C.
dc.contributor.authorBruce, Kat
dc.contributor.authorBrys, Rein
dc.contributor.authorHalfmaerten, David
dc.contributor.authorRead, Daniel S.
dc.contributor.authorWatson, Hayley V.
dc.contributor.authorSayer, Carl D.
dc.contributor.authorJones, Elanor P.
dc.contributor.authorPriestley, Victoria
dc.contributor.authorMächler, Elvira
dc.contributor.authorMúrria, Cesc
dc.contributor.authorGarcès-Pastor, Sandra
dc.contributor.authorMedupin, Cecilia
dc.contributor.authorBurgess, Katherine
dc.contributor.authorBenson, Gillian
dc.contributor.authorBoonham, Neil
dc.contributor.authorGriffiths, Richard A.
dc.contributor.authorHandley, Lori Lawson
dc.contributor.authorHänfling, Bernd
dc.date.accessioned2019-05-16T12:08:40Z
dc.date.available2019-05-16T12:08:40Z
dc.date.issued2018-09-03
dc.description.abstractEnvironmental DNA (eDNA) analysis is a rapid, non-invasive, cost-efficient biodiversity monitoring tool with enormous potential to inform aquatic conservation and management. Development is ongoing, with strong commercial interest, and new uses are continually being discovered. General applications of eDNA and guidelines for best practice in freshwater systems have been established, but habitat-specific assessments are lacking. Ponds are highly diverse, yet understudied systems that could benefit from eDNA monitoring. However, eDNA applications in ponds and methodological constraints specific to these environments remain unaddressed. Following a stakeholder workshop in 2017, researchers combined knowledge and expertise to review these applications and challenges that must be addressed for the future and consistency of eDNA monitoring in ponds. The greatest challenges for pond eDNA surveys are representative sampling, eDNA capture, and potential PCR inhibition. We provide recommendations for sampling, eDNA capture, inhibition testing, and laboratory practice, which should aid new and ongoing eDNA projects in ponds. If implemented, these recommendations will contribute towards an eventual broad standardisation of eDNA research and practice, with room to tailor workflows for optimal analysis and different applications. Such standardisation will provide more robust, comparable, and ecologically meaningful data to enable effective conservation and management of pond biodiversity.en_US
dc.descriptionSource at <a href=https://doi.org/10.1007/s10750-018-3750-5>https://doi.org/10.1007/s10750-018-3750-5. </a>en_US
dc.identifier.citationHarper, L.R., Buxton, A.S., Rees, H.S., Bruce, K., Brys, R., Halfmaerten, D. ... Hänfling, B. (2018). Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds. <i>Hydrobiologia, 826</i>(1), 25-41. https://doi.org/10.1007/s10750-018-3750-5en_US
dc.identifier.cristinIDFRIDAID 1621673
dc.identifier.doi10.1007/s10750-018-3750-5
dc.identifier.issn0018-8158
dc.identifier.issn1573-5117
dc.identifier.urihttps://hdl.handle.net/10037/15320
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.journalHydrobiologia
dc.rights.accessRightsopenAccessen_US
dc.subjectAquaticen_US
dc.subjectBiodiversityen_US
dc.subjectLenticen_US
dc.subjectMetabarcodingen_US
dc.subjectQuantitativeen_US
dc.subjectPCRen_US
dc.subjectSurveyen_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titleProspects and challenges of environmental DNA (eDNA) monitoring in freshwater pondsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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