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dc.contributor.authorWutkowska, Magdalena
dc.contributor.authorVader, Anna
dc.contributor.authorMundra, Sunil
dc.contributor.authorCooper, Elisabeth J.
dc.contributor.authorEidesen, Pernille Bronken
dc.date.accessioned2019-08-14T14:01:59Z
dc.date.available2019-08-14T14:01:59Z
dc.date.issued2019-01-08
dc.description.abstractDescribing dynamics of belowground organisms, such as fungi, can be challenging. Results of studies based on environmental DNA (eDNA) may be biased as the template does not discriminate between metabolically active cells and dead biomass. We analyzed ribosomal DNA (rDNA) and ribosomal RNA (rRNA) coextracted from 48 soil samples collected from a manipulated snow depth experiment in two distinct vegetation types in Svalbard, in the High Arctic. Our main goal was to compare if the rDNA and rRNA metabarcoding templates produced congruent results that would lead to consistent ecological interpretation. Data derived from both rDNA and rRNA clustered according to vegetation types. Different sets of environmental variables explained the community composition based on the metabarcoding template. rDNA and rRNA-derived community composition of symbiotrophs and saprotrophs, unlike pathotrophs, clustered together in a similar way as when the community composition was analyzed using all OTUs in the study. Mean OTU richness was higher for rRNA, especially in symbiotrophs. The metabarcoding template was more important than vegetation type in explaining differences in richness. The proportion of symbiotrophic, saprotrophic and functionally unassigned reads differed between rDNA and rRNA, but showed similar trends. There was no evidence for increased snow depth influence on fungal community composition or richness. Our findings suggest that template choice may be especially important for estimating biodiversity, such as richness and relative abundances, especially in Helotiales and Agaricales, but not for inferring community composition. Differences in study results originating from rDNA or rRNA may directly impact the ecological conclusions of one’s study, which could potentially lead to false conclusions on the dynamics of microbial communities in a rapidly changing Arctic.en_US
dc.description.sponsorshipNorthern Area Program - ConocoPhillips and Lundin Petroleum Publication fund of UiT The Arctic University of Norwayen_US
dc.identifier.citationWutkowska, M., Vader, A., Mundra, S., Cooper, E..J. & Eidesen, P.B. (2019). Dead or Alive; or Does It Really Matter? Level of Congruency Between Trophic Modes in Total and Active Fungal Communities in High Arctic Soil. <i>Frontiers in Microbiology, 9</i>, 3243. https://doi.org/10.3389/fmicb.2018.03243en_US
dc.identifier.cristinIDFRIDAID 1656110
dc.identifier.doi10.3389/fmicb.2018.03243
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/10037/15914
dc.language.isoengen_US
dc.publisherFrontiersen_US
dc.relation.ispartofWutkowska, M. (2020). Microbial eukaryotes and their functional importance in the Arctic. A Svalbardian perspective. (Doctoral thesis). <a href=https://hdl.handle.net/10037/19552>https://hdl.handle.net/10037/19552</a>.
dc.relation.journalFrontiers in Microbiology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/230970/Norway/The effect of snow depth and snow melt timing on arctic terrestrial ecosystems//en_US
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.03243/full
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472en_US
dc.subjectbelow-ground processesen_US
dc.subjectfungal trophic modeen_US
dc.subjectfungal functional groupen_US
dc.subjectsnow regimeen_US
dc.subjectarctic vegetationen_US
dc.subjectsnow fencesen_US
dc.titleDead or Alive; or Does It Really Matter? Level of Congruency Between Trophic Modes in Total and Active Fungal Communities in High Arctic Soilen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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