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dc.contributor.authorPenaranda, Ma Michelle Demogina
dc.contributor.authorJensen, Ingvill
dc.contributor.authorTollersrud, Linn G.
dc.contributor.authorBruun, Jack-Ansgar
dc.contributor.authorJørgensen, Jorunn B.
dc.date.accessioned2019-10-25T10:41:06Z
dc.date.available2019-10-25T10:41:06Z
dc.date.issued2019-01-29
dc.description.abstractFish immunology research is at a pivotal point with the increasing availability of functional immunoassays and major advances in <i>omics</i> approaches. However, studies on fish B cells and their distinct subsets remain a challenge due to the limited availability of differentially expressed surface markers. To address this constraint, cell surface proteome of Atlantic salmon IgM<sup>+</sup> B cells were analyzed by mass spectrometry and compared to surface proteins detected from two adherent salmon head kidney cell lines, ASK and SSP-9. Out of 21 cluster of differentiation (CD) molecules identified on salmon IgM<sup>+</sup> B cells, CD22 and CD79A were shortlisted as potential markers based on the reported B cell-specific surface expression of their mammalian homologs. Subsequent RT-qPCR analyses of flow cytometry-sorted subpopulations from head kidney leukocytes confirmed that both <i>cd22</i> and <i>cd79a</i> genes were highly expressed in IgM<sup>+</sup> lymphoid cells but were observed in barely detectable levels in IgM<sup>−</sup> non-lymphoid suspension and adherent cells. Similarly, significantly high <i>cd22</i> and <i>cd79a</i> mRNA levels were observed in IgM<sup>+</sup> or IgT<sup>+</sup> lymphoid cells from the spleen and peritoneal cavity, but not in their corresponding IgM<sup>−</sup> IgT<sup>−</sup> non-lymphoid fractions. This suggests that the B cell restrictive expression of CD22 and CD79A extend down to the transcription level, which was consistent across different lymphoid compartments and immunoglobulin isotypes, thus strongly supporting the potential of CD22 and CD79A as pan-B cell markers for salmon. In addition, this study provides novel information on the salmon B cell surface protein repertoire, as well as insights on B cell evolution. Further investigation of the identified salmon CD molecules, including development of immunological tools for detection, will help advance our understanding of the dynamics of salmon B cell responses such as during infection, vaccination, or immunostimulation.en_US
dc.descriptionSource at <a href=https://doi.org/10.3389/fimmu.2019.00037>https://doi.org/10.3389/fimmu.2019.00037</a>.en_US
dc.identifier.citationPeñaranda, M.M.D., Jensen, I., Bruun, J.A. & Jørgensen, J.B. (2019). Profiling the Atlantic Salmon IgM+ B Cell Surface Proteome: Novel Information on Teleost Fish B Cell Protein Repertoire and Identification of Potential B Cell Markers. <i>Frontiers in Immunology, 10</i>, 37. https://doi.org/10.3389/fimmu.2019.00037en_US
dc.identifier.cristinIDFRIDAID 1732369
dc.identifier.doi10.3389/fimmu.2019.00037
dc.identifier.issn1664-3224
dc.identifier.urihttps://hdl.handle.net/10037/16473
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Immunology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/245892/Norway/Multiple routes to B cell memory in Atlantic salmon//en_US
dc.relation.urihttps://doi.org/10.3389/fimmu.2019.00037
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473en_US
dc.subjectB cellsen_US
dc.subjectcell surface markersen_US
dc.subjectteleost fishen_US
dc.subjectsalmonen_US
dc.subjectCD22en_US
dc.subjectCD79Aen_US
dc.subjectIgMen_US
dc.subjectproteomicsen_US
dc.titleProfiling the Atlantic Salmon IgM+ B Cell Surface Proteome: Novel Information on Teleost Fish B Cell Protein Repertoire and Identification of Potential B Cell Markersen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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