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dc.contributor.authorGu, Yuexi
dc.contributor.authorAbudu, Yakubu Princely
dc.contributor.authorKumar, Suresh
dc.contributor.authorBissa, Bhawana
dc.contributor.authorChoi, Seong Won
dc.contributor.authorJia, Jingyue
dc.contributor.authorLazarou, Michael
dc.contributor.authorEskelinen, Eeva-Liisa
dc.contributor.authorJohansen, Terje
dc.contributor.authorDeretic, Vojo
dc.date.accessioned2020-03-16T13:00:47Z
dc.date.available2020-03-16T13:00:47Z
dc.date.issued2019-10-18
dc.description.abstractMammalian homologs of yeast Atg8 protein (mAtg8s) are important in autophagy, but their exact mode of action remains ill‐defined. Syntaxin 17 (Stx17), a SNARE with major roles in autophagy, was recently shown to bind mAtg8s. Here, we identified LC3‐interacting regions (LIRs) in several SNAREs that broaden the landscape of the mAtg8‐SNARE interactions. We found that Syntaxin 16 (Stx16) and its cognate SNARE partners all have LIR motifs and bind mAtg8s. Knockout of Stx16 caused defects in lysosome biogenesis, whereas a Stx16 and Stx17 double knockout completely blocked autophagic flux and decreased mitophagy, pexophagy, xenophagy, and ribophagy. Mechanistic analyses revealed that mAtg8s and Stx16 control several properties of lysosomal compartments including their function as platforms for active mTOR. These findings reveal a broad direct interaction of mAtg8s with SNAREs with impact on membrane remodeling in eukaryotic cells and expand the roles of mAtg8s to lysosome biogenesis.en_US
dc.identifier.citationGu, Y., Abudu, Y.P., Kumar, S., Bissa, B., Choi, S.W., Jia, J. ... Deretic, V. (2019). Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs. <i>EMBO Journal, 38</i>(22): e101994).en_US
dc.identifier.cristinIDFRIDAID 1738387
dc.identifier.doi10.15252/embj.2019101994
dc.identifier.issn0261-4189
dc.identifier.issn1460-2075
dc.identifier.urihttps://hdl.handle.net/10037/17755
dc.language.isoengen_US
dc.publisherEMBO Pressen_US
dc.relation.journalEMBO Journal
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/249884/Norway/Autophagy-regulated Signalosomes in Cellular Stress and Disease Pathways//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titleMammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREsen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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