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dc.contributor.authorØie, Cristina Ionica
dc.contributor.authorWolfson, Deanna
dc.contributor.authorYasunori, Tanji
dc.contributor.authorDumitriu, Gianina
dc.contributor.authorSørensen, Karen Kristine
dc.contributor.authorMcCourt, Peter Anthony
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorSmedsrød, Bård
dc.date.accessioned2020-03-24T11:07:53Z
dc.date.available2020-03-24T11:07:53Z
dc.date.issued2020-01-21
dc.description.abstractThe liver is constantly exposed to dietary antigens, viruses, and bacterial products with inflammatory potential. For decades cellular uptake of virus has been studied in connection with infection, while the few studies designed to look into clearance mechanisms focused mainly on the role of macrophages. In recent years, attention has been directed towards the liver sinusoidal endothelial cells (LSECs), which play a central role in liver innate immunity by their ability to scavenge pathogen- and damage-associated molecular patterns. Every day our bodies are exposed to billions of gut-derived pathogens which must be efficiently removed from the circulation to prevent inflammatory and/or immune reactions in other vascular beds. Here, we have used GFP-labelled Enterobacteria phage T4 (GFP-T4-phage) as a model virus to study the viral scavenging function and metabolism in LSECs. The uptake of GFP-T4-phages was followed in real-time using deconvolution microscopy, and LSEC identity confirmed by visualization of fenestrae using structured illumination microscopy. By combining these imaging modalities with quantitative uptake and inhibition studies of radiolabelled GFP-T4-phages, we demonstrate that the bacteriophages are effectively degraded in the lysosomal compartment. Due to their high ability to take up and degrade circulating bacteriophages the LSECs may act as a primary anti-viral defence mechanism.en_US
dc.identifier.citationØie CI, Wolfson DL, Yasunori T, Dumitriu GA, Sørensen KK, McCourt PAG, Ahluwalia BS, Smedsrød b. Liver sinusoidal endothelial cells contribute to the uptake and degradation of entero bacterial viruses.. Scientific Reports. 2020en_US
dc.identifier.cristinIDFRIDAID 1789957
dc.identifier.doi10.1038/s41598-020-57652-0
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/17837
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.relation.journalScientific Reports
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7-IDEAS-ERC/336716/EU/High-speed chip-based nanoscopy to discover real-time sub-cellular dynamics/NANOSCOPY/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMED2/262538/Norway/Role of scavenger endothelial cells in elimination of virus//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titleLiver sinusoidal endothelial cells contribute to the uptake and degradation of entero bacterial virusesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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