dc.contributor.author | Hegstad, Kristin | |
dc.contributor.author | Mylvaganam, Haima | |
dc.contributor.author | Janice, Jessin | |
dc.contributor.author | Josefsen, Ellen H. | |
dc.contributor.author | Sivertsen, Audun | |
dc.contributor.author | Skaare, Dagfinn | |
dc.date.accessioned | 2020-06-09T11:14:47Z | |
dc.date.available | 2020-06-09T11:14:47Z | |
dc.date.issued | 2020-01-29 | |
dc.description.abstract | <i>Haemophilus influenzae</i> colonizes the respiratory tract in humans and causes both invasive and noninvasive infections. Resistance to extended-spectrum cephalosporins in <i>H. influenzae</i> is rare in Europe. In this study, we defined acquired resistance gene loci and <i>ftsI</i> mutations in multidrug-resistant (MDR) and/or PBP3-mediated beta-lactam-resistant (rPBP3) <i>H. influenzae</i> strains, intending to understand the mode of spread of antibiotic resistance determinants in this species. Horizontal transfer of mobile genetic elements and transformation with resistance-conferring <i>ftsI</i> alleles were contributory. We found one small plasmid and three novel integrative conjugative elements (ICEs) which carry different combinations of resistance genes. Demonstration of transfer and/or ICE circular forms showed that the ICEs are functional. Two extensively MDR genetically unrelated <i>H. influenzae</i> strains (F and G) from the same geographical region shared an identical novel MDR ICE (Tn<i>6686</i>) harboring <i>bla</i><sub>TEM-1</sub>, <i>catA2</i>-like, and <i>tet</i>(B). The first Nordic case of MDR <i>H. influenzae</i> septicemia, strain 0, originating from the same geographical area as these strains, had a similar resistance pattern but contained another ICE [Tn<i>6687</i> with <i>bla</i><sub>TEM-1</sub>, <i>catP</i> and <i>tet</i>(B)] with an overall structure quite similar to that of Tn<i>6686</i>. Comparison of the complete <i>ftsI</i> genes among rPBP3 strains revealed that the entire gene or certain regions of it are identical in genetically unrelated strains, indicating horizontal gene transfer. Our findings illustrate that <i>H. influenzae</i> is capable of acquiring resistance against a wide range of commonly used antibiotics through horizontal gene transfer, in terms of conjugative transfer of ICEs and transformation of chromosomal genes. | en_US |
dc.identifier.citation | Hegstad K, Mylvaganam H, Janice JJ, Josefsen EH, Sivertsen A, Skaare D. Role of Horizontal Gene Transfer in the Development of Multidrug Resistance in Haemophilus influenzae. mSphere. 2020;5(1) | en_US |
dc.identifier.cristinID | FRIDAID 1790997 | |
dc.identifier.doi | 10.1128/mSphere.00969-19 | |
dc.identifier.issn | 2379-5042 | |
dc.identifier.uri | https://hdl.handle.net/10037/18499 | |
dc.language.iso | eng | en_US |
dc.publisher | American Society for Microbiology | en_US |
dc.relation.journal | mSphere | |
dc.relation.uri | https://msphere.asm.org/content/msph/5/1/e00969-19.full.pdf | |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2020 The Author(s) | en_US |
dc.subject | VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710 | en_US |
dc.subject | VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710 | en_US |
dc.title | Role of Horizontal Gene Transfer in the Development of Multidrug Resistance in Haemophilus influenzae | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |