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dc.contributor.authorDiab, Joseph
dc.contributor.authorAl-Mahdi, Rania
dc.contributor.authorGouveia, Sandra
dc.contributor.authorHansen, Terkel
dc.contributor.authorJensen, Einar
dc.contributor.authorGoll, Rasmus
dc.contributor.authorMoritz, Thomas
dc.contributor.authorFlorholmen, Jon
dc.contributor.authorForsdahl, Guro
dc.date.accessioned2020-09-22T07:04:09Z
dc.date.available2020-09-22T07:04:09Z
dc.date.issued2018-11-22
dc.description.abstract<p><i>Background</i> - The bioactive metabolites of omega 3 and omega 6 polyunsaturated fatty acids (ω-3 and ω-6) are known as oxylipins and endocannabinoids (eCBs). These lipid metabolites are involved in prompting and resolving the inflammatory response that leads to the onset of inflammatory bowel disease (IBD). This study aims to quantify these bioactive lipids in the colonic mucosa and to evaluate the potential link to cytokine gene expression during inflammatory events in ulcerative colitis (UC). <p><i>Methods</i> - Colon biopsies were taken from 15 treatment-naïve UC patients, 5 deep remission UC patients, and 10 healthy controls. Thirty-five oxylipins and 11 eCBs were quantified by means of ultra-high-performance liquid chromatography coupled with tandem mass spectrometry. Levels of mRNA for 10 cytokines were measured by reverse transcription polymerase chain reaction. <p><i>Results</i> - Levels of ω-6-related oxylipins were significantly elevated in treatment-naïve patients with respect to controls, whereas the levels of ω-3 eCBs were lower. 15S-Hydroxy-eicosatrienoic acid (15S-HETrE) was significantly upregulated in UC deep remission patients compared with controls. All investigated cytokines had significantly higher mRNA levels in the inflamed mucosa of treatment-naïve UC patients. Cytokine gene expression was positively correlated with several ω-6 arachidonic acid–related oxylipins, whereas negative correlation was found with lipoxin, prostacyclin, and the eCBs. <p><i>Conclusions</i> - Increased levels of ω-6-related oxylipins and decreased levels of ω-3-related eCBs are associated with the debut of UC. This highlights the altered balance between pro- and anti-inflammatory lipid mediators in IBD and suggests potential targets for intervention.en_US
dc.identifier.citationDiab J, Al-Mahdi R, Gouveia S, Hansen T, Jensen e, Goll r, Moritz T, Florholmen J, Forsdahl G. A Quantitative Analysis of Colonic Mucosal Oxylipins and Endocannabinoids in Treatment-Naïve and Deep Remission Ulcerative Colitis Patients and the Potential Link With Cytokine Gene Expression. Inflammatory Bowel Diseases. 2018;25(3):490-497en_US
dc.identifier.cristinIDFRIDAID 1638432
dc.identifier.doi10.1093/ibd/izy349
dc.identifier.issn1078-0998
dc.identifier.issn1536-4844
dc.identifier.urihttps://hdl.handle.net/10037/19447
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofDiab, J. (2020). The Metabolome and Lipidome of Ulcerative Colitis. (Doctoral thesis). <a href=https://hdl.handle.net/10037/19449>https://hdl.handle.net/10037/19449</a>.
dc.relation.journalInflammatory Bowel Diseases
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2018 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Gastroenterology: 773en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Gasteroenterologi: 773en_US
dc.titleA Quantitative Analysis of Colonic Mucosal Oxylipins and Endocannabinoids in Treatment-Naïve and Deep Remission Ulcerative Colitis Patients and the Potential Link With Cytokine Gene Expressionen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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