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dc.contributor.authorTeige, Lena Hammerlund
dc.contributor.authorAksnes, Ida
dc.contributor.authorRøsæg, Magnus Vikan
dc.contributor.authorJensen, Ingvill
dc.contributor.authorJørgensen, Jorunn
dc.contributor.authorSindre, Hilde
dc.contributor.authorCollins, Catherine
dc.contributor.authorCollet, Bertrand
dc.contributor.authorRimstad, Espen
dc.contributor.authorDahle, Maria
dc.contributor.authorBoysen, Preben
dc.date.accessioned2020-10-29T09:21:46Z
dc.date.available2020-10-29T09:21:46Z
dc.date.issued2020-07-30
dc.description.abstractSalmonid alphavirus (SAV) is the etiological cause of pancreas disease (PD) in Atlantic salmon (<i>Salmo salar</i>). Several vaccines against SAV are in use, but PD still cause significant mortality and concern in European aquaculture, raising the need for optimal tools to monitor SAV immunity. To monitor and control the distribution of PD in Norway, all salmonid farms are regularly screened for SAV by RT-qPCR. While the direct detection of SAV is helpful in the early stages of infection, serological methods could bring additional information on acquired SAV immunity in the later stages. Traditionally, SAV antibodies are monitored in neutralization assays, but they are time-consuming and cumbersome, thus alternative assays are warranted. Enzyme-linked immunosorbent assays (ELISAs) have not yet been successfully used for anti-SAV antibody detection in aquaculture. We aimed to develop a bead-based immunoassay for SAV-specific antibodies. By using detergent-treated SAV particles as antigens, we detected SAV-specific antibodies in plasma collected from both a SAV challenge trial and a field outbreak of PD. Increased levels of SAV-specific antibodies were seen after most fish had become negative for viral RNA. The bead-based assay is time saving compared to virus neutralization assays, and suitable for non-lethal testing due to low sample size requirements. We conclude that the bead-based immunoassay for SAV antibody detection is a promising diagnostic tool to complement SAV screening in aquaculture.en_US
dc.identifier.citationTeige, Aksnes, Røsæg, Jensen, Jørgensen, Sindre, Collins, Collet, Rimstad, Dahle, Boysen. Detection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassay. Fish and Shellfish Immunology. 2020en_US
dc.identifier.cristinIDFRIDAID 1824581
dc.identifier.doi10.1016/j.fsi.2020.07.055
dc.identifier.issn1050-4648
dc.identifier.issn1095-9947
dc.identifier.urihttps://hdl.handle.net/10037/19701
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalFish and Shellfish Immunology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2?/280847/Norway/?/ViVaAct?/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.titleDetection of specific Atlantic salmon antibodies against salmonid alphavirus using a bead-based immunoassayen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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