Now showing items 51-70 of 76

    • Non-Invasive Methods for Detection of Genetic Abnormalities in Human Embryos Cell-Free DNA Secreted from Human Embryos as an Alternative to DNA from Cell Biopsies for Genetic Analyses 

      Finanger, Viktoria Emeline Swaty (Mastergradsoppgave; Master thesis, 2022-05-15)
      Chromosomal abnormalities are one of the main causes of implantation failure following embryo transfer in in vitro fertilization (IVF). Preimplantation genetic testing for aneuploidies (PGT-A) is time-consuming, expensive, and invasive. Non-invasive PGT-A (niPGT-A) is an alternative where secreted embryonic cell-free DNA (cfDNA) in spent culture medium (SCM) is investigated. This study aimed to ...
    • Oncolytic Activity and Mechanisms of Action by a small β2,2-amino acid derivative LTX-401 

      Mauseth, Brynjar (Master thesis; Mastergradsoppgave, 2015-05-11)
      Antimicrobial peptides are part of the innate immune defence of many organisms and represent a novel class of therapeutics due to their broad-spectrum activities, including cytotoxic activity against cancer cells. It has recently been reported that small antimicrobial β2,2-amino acid derivatives (Mw<500), such as LTX-401, possess potent anticancer activity against Ramos human Burkitt’s lymphoma ...
    • The oncoproteins gelsolin, periostin and thrombospondin are enriched in Merkel cell carcinoma exosomes, and their promoter activity is stimulated by Large T-antigen of Merkel cell polyomavirus 

      Furuholmen-Jenssen, Ida Sofie (Master thesis; Mastergradsoppgave, 2017-05-14)
      Merkel cell carcinoma (MCC) is a rare, aggressive neuroendocrine form of skin cancer with high mortality rate and increasing incidence. Around 80 % of MCC tumours are positive for Merkel cell polyomavirus (MCPyV), the only polyomavirus known to induce cancer in humans. Continuous expression of the viral oncogenes LT-ag and st-ag is necessary for MCPyV positive MCC tumour cell survival, suggesting ...
    • Optimisation of high-resolution fluorescence microscopy of normal- and preeclamptic placentas 

      Knutsen, Kristine Marie Bakken (Master thesis; Mastergradsoppgave, 2019-08-14)
      Preeclampsia (PE) affects 3-5 % of pregnant women and may lead to maternal and/or fetal death. The main theory of PE is placental ischemia, leading to a dysfunctional placenta and clinical signs as hypertension and proteinuria in the mother. The primary aim of the thesis was to implement and optimise a method for high-resolution microscopy of placental cryo-sections. Secondary aims were to compare ...
    • Optimization of biomarkers for morphological analysis of healthy and preeclamptic term human placental tissue sections using advanced fluorescence microscopy methods 

      Nalliah, Maddhusja (Mastergradsoppgave; Master thesis, 2021-07-01)
      Preeclampsia (PE) is a pregnancy-related disorder affecting 5-8% of women worldwide (4% in Norway). It is believed that placental ischemia is the initial event in the development of PE and is characterized by placental insufficiency and clinical symptoms such as hypertension and proteinuria. In this project, we aimed to study suitable biomarkers for morphological analysis of human term placenta from ...
    • Optimization of the use of PrepFiler Express™ Forensic DNA extraction kit lysis buffer discard for mRNA profiling 

      Hamnevoll, Kari (Mastergradsoppgave; Master thesis, 2021-06-01)
      mRNA profiling for body fluid or tissue type identification is able to provide circumstantial information alongside DNA profiles from biological material found at a crime scene. To solve the challenge of material limitation in body fluid identification, studies have successfully developed mRNA profiles of RNA extracted from lysis buffer discard of DNA-extraction. It would be interesting to examine ...
    • Partial characterization of predicted ABCC5 inhibitors by the aid of human erythrocyte inside-out vesicles 

      Ørvoll, Elin (Master thesis; Mastergradsoppgave, 2011-06-24)
      ABCC5 is a member of the superfamily of ABC-transporters, and it has been identified as an efflux transporter of cGMP. This transporter is also involved in export of antibiotic and cytostatic drugs from target cells, and as such represents a challenge in treatment of cancer and infectious diseases. In order to find inhibitors to ABCC5 mediated drug efflux, compounds predicted as potent inhibitors ...
    • The population structure of human carriage and clinical isolates of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Norway 

      Andreassen, Lotte (Master thesis; Mastergradsoppgave, 2017-05-15)
      The increasing emergence of antimicrobial resistant bacteria worldwide is recognised as a severe threat to public health on a global scale. Without effective antimicrobial agents to treat bacterial infections, modern medicine will be set back several decades and deaths caused by bacterial infections will increase. The most widely used class of antimicrobials, is -lactams, and the increase in resistance ...
    • Prevalence of plasmid-mediated quinolone resistance in Norwegian and Swedish clinical isolates of Escherchia coli and Klebsiella spp. 

      Karah, Nabil (Master thesis; Mastergradsoppgave, 2008-06-12)
      The aim of this study was to examine the presence of plasmid-mediated qnr and aac(6')-Ib-cr resistance genes among Escherichia coli and Klebsiella spp. clinical isolates obtained between 2003 and 2005 from Norway and Sweden and showed resistance and/or reduced susceptibility to nalidixic acid and ciprofloxacin, respectively. Prevalence of the qnr (1.6%) and aac(6’)-Ib-cr (14.1%) determinants was ...
    • Production and purification of recombinant C-terminal truncated pro-Matrix Metalloprotease-9 

      Rahman, Fatema (Master thesis; Mastergradsoppgave, 2016-06-21)
      MMP-9 consists of N-terminal signal peptide, a pro-domain, catalytic domain, a long flexible hinge region and C- terminal hemopexin domain. In the present study a truncated version of proMMP-9 which lacking hinge region and C-terminal hemopexin domain was produced. To do so Sf9 insect cells and the BaculoDirect Baculovirus expression system were used. Using site directed mutagenesis, single mutation ...
    • The prognostic significance of miR-17-5p and miR-20a-5p in prostate cancer 

      Ingebriktsen, Lise Martine (Mastergradsoppgave; Master thesis, 2019-05-15)
      Prostate cancer accounts for extensive mortality and is the second most frequent cancer type occurring in men, acknowledged as a severe health problem globally. In Norway, Prostate cancer is the most common form of cancer in men, and around 5.000 patients are diagnosed each year. Using in situ hybridization, we examined the in situ tissue distribution of miR-17-5p and miR-20a-5p in prostate cancer ...
    • Real-time polymerase chain reaction species specific for Neisseria Gonorrhoeae 

      Olsen, Merethe Elise (Master thesis; Mastergradsoppgave, 2008-05)
      Gonorrhea is a sexually transmitted disease caused by bacteria called gonococci. The disease was described in ancient times, but until the late nineteenth century it was mixed up with syphilis. Eventually microscopy could differ between the organisms and give a reliable diagnosis. Human is the only known reservoir for gonorrhea. It is transferred during all acts of sexual activity, and an infection ...
    • Regulation of urokinase receptor (uPAR) cleavage by cancer associated fibroblasts (CAFs) in oral cancer 

      Kitaw, Manyahilishal Etana (Master thesis; Mastergradsoppgave, 2019-05-15)
      Oral squamous cell carcinoma (OSCC) is one of the frequently diagnosed type of oral cancers and is a leading cause of cancer associated mortality and morbidity worldwide. Cancer associated fibroblasts (CAFs) are activated fibroblasts that are found in association with cancer cells. CAFs are the most abundant stromal cells in the tumor microenvironment (TME). In the TME, cell interactions mediated ...
    • The Regulatory Relationship between E2F4 and MiRNA-363, and it’s Relevance to Oral Cancer 

      Assadi, Geed Alaa (Master thesis; Mastergradsoppgave, 2020-08-14)
      BACKGROUND Oral Squamous Cell Carcinoma (OSCC) is the most common type of head and neck cancer and persists a leading cause of cancer associated mortality and morbidity universally. Survival rate is still poor at less than 50% urging the need for biomarkers to allow better diagnosis, prognosis and therapeutic strategies. In this study we focus on E2F4, a repressor of cell cycle, and further propose ...
    • The role of MMP-2 and MMP-14 in cell migration and invasion 

      Okoli, Ndidiamaka Chinelo (Master thesis; Mastergradsoppgave, 2014-02-03)
      Cancer is becoming a global epidemic in recent times and can affect different organs in the body and even more worrying is that they do not have a common cause. Several factors have been implicated in the onset and spread of cancers, one of which are the metalloproteinases (MMPs). The MMPs utilize the same strategy they employ in normal physiological processes in pathology, thus creating a dilemma ...
    • Role of the mannose receptor in tumor associated macrophages. Studies in a mouse melanoma model 

      Rønningen, Iselin (Master thesis; Mastergradsoppgave, 2014-04-15)
      Tumor-associated macrophages (TAMs) play an important role in cancer-related inflammation. TAMs develop M2 polarization which may support tumor growth. Mannose receptor (MR) expression is a hallmark for this cell-subpopulation, and the MR has recently been suggested to be involved in TAM polarization. The aim of the study was to investigate the role of MR in TAM polarization and tumor development ...
    • Study of human PRKAR1A and its role in autophagy 

      Lamsal, Apsana (Master thesis; Mastergradsoppgave, 2018-05-11)
      The human PRKAR1A gene is a 381- amino acid protein encoding the regulatory subunit (RIα) of the cAMP dependent Protein Kinase A (PKA). The main function of PRKAR1A is to regulate the catalytic activity of PKA. PRKAR1A is shown to regulate autophagy via association with mTOR kinase and colocalization with the autophagy marker proteins LC3B, Rab7 and Rab9. Contradictory findings regarding the ...
    • Study of interaction between BK virus large T-antigen and agnoprotein 

      Adou, Koman Mireille Sophie Chinan (Master thesis; Mastergradsoppgave, 2011-08)
      Human polyomavirus BK (BKV) is a non enveloped virus with a double-stranded, circular DNA genome. BKV infects >70% of the human population world-wide. Infection occurs predominantly during childhood and the virus remains in a latent state throughout life in the immune competent individuals. In the context of immunosuppression, however, reactivation occurs and can lead to renal stenosis and interstitial ...
    • A study of possible genetic causes of inherited breast and ovarian cancer in a Norwegian cancer population 

      Jarhelle, Elisabeth (Master thesis; Mastergradsoppgave, 2013-05-15)
      Homologous recombination repair (HRR) is an important repair mechanism, and mutations disrupting the function of this machinery might contribute to cancer formation. Several proteins interact in this mechanism, and the two best known are BRCA1 and BRCA2. Mutations in their corresponding genes BRCA1 and BRCA2 are found in 40% of hereditary breast and ovarian cancer cases. However, there are still ...
    • A study of the interaction between MAPKAP Kinase 5 / MK5 and DNAJB1 

      Lægreid, Kari Jenssen (Master thesis; Mastergradsoppgave, 2011-05-02)
      The mitogen activated protein kinases (MAPK) are a large and diverse family of protein kinases, contributing to the cells ability to respond to external stimuli by relaying messages in a well orchestrated way until they reach their final destinations. This is achieved through successive phosphorylation events. One member of this large family is mitogen activated protein kinase activated protein ...