Blar i forfatter Mastergradsoppgaver i biologi (Helsefak) "Bohra, Pallavi"
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Directed evolution of Escherichia coli LacZ gene to create diversity in glycosidic bonds hydrolysis
Bohra, Pallavi (Master thesis; Mastergradsoppgave, 2011-05-16)Starting with LacZ of Escherichia coli, coding for β-galactosidase, the aim of the thesis project is to apply in vitro directed evolution techniques to help create other glycosidic bond hydrolysis activities. This was done using the main β-galactosidase backbone with limited amino acid sequence change. Any altered glycosyl hydrolase activity would lead to changed substrate specificity. Moreover, ...