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In vitro reconstitution of proMatrix Metalloproteinase-9/Chondroitin Sulfate Proteoglycan Complexes. Identification of motifs in proMMP-9 and the serglycin core protein involved in the complex formation.
(Master thesis; Mastergradsoppgave, 2014-05-15)
Previously it has been shown that different monocytic leukemic cell lines such as THP-1, MonoMac and U-937 can produce proteolytic enzymes such as MMP-9 as well as various types of proteoglycans (PG). These proteins have been shown to be involved in homeostasis as well as in various diseases, such as cancer. When proMMP-9 was mixed with isolated PGs from these three cell lines or pure serglycin and ...
The oncoproteins gelsolin, periostin and thrombospondin are enriched in Merkel cell carcinoma exosomes, and their promoter activity is stimulated by Large T-antigen of Merkel cell polyomavirus
(Master thesis; Mastergradsoppgave, 2017-05-14)
Merkel cell carcinoma (MCC) is a rare, aggressive neuroendocrine form of skin cancer with high mortality rate and increasing incidence. Around 80 % of MCC tumours are positive for Merkel cell polyomavirus (MCPyV), the only polyomavirus known to induce cancer in humans. Continuous expression of the viral oncogenes LT-ag and st-ag is necessary for MCPyV positive MCC tumour cell survival, suggesting ...
Role of the mannose receptor in tumor associated macrophages. Studies in a mouse melanoma model
(Master thesis; Mastergradsoppgave, 2014-04-15)
Tumor-associated macrophages (TAMs) play an important role in cancer-related inflammation. TAMs develop M2 polarization which may support tumor growth. Mannose receptor (MR) expression is a hallmark for this cell-subpopulation, and the MR has recently been suggested to be involved in TAM polarization. The aim of the study was to investigate the role of MR in TAM polarization and tumor development ...
Molecular characterization of Norwegian clinical isolates of Escherichia coli hyperproducing the chromosomal AmpC beta-lactamase : a regional spread of an IS911-mediated blaAmpC-hyperexpressing ST131 clone
(Master thesis; Mastergradsoppgave, 2012-05-15)
The worldwide dissemination of antimicrobial resistance is a growing problem causing increased morbidity, mortality, and financial costs. β-lactams are an important family of antimicrobial agents and accounts for ~46% of the total antibiotic use for systemic infections in Norway. Resistance to β-lactams can be caused by several factors where the production of enzymes, β-lactamases, is the major ...
A study of the protein kinase MK5's effect on melanoma cell proliferation
(Master thesis; Mastergradsoppgave, 2012-09-14)
The mitogen-activated protein kinases (MAPKs) play a crucial role in cellular processes such as proliferation, differentiation, apoptosis, metabolism and motility. Both conventional and atypical MAPKs can phosphorylate other proteins referred to as MAPK- activated protein kinases (MAPKAPK) such as MK5 or p38-regulated /activated protein kinase. Mutations in the MK5 gene have been detected in lung, ...
Partial characterization of predicted ABCC5 inhibitors by the aid of human erythrocyte inside-out vesicles
(Master thesis; Mastergradsoppgave, 2011-06-24)
ABCC5 is a member of the superfamily of ABC-transporters, and it has been identified as an efflux transporter of cGMP. This transporter is also involved in export of antibiotic and cytostatic drugs from target cells, and as such represents a challenge in treatment of cancer and infectious diseases. In order to find inhibitors to ABCC5 mediated drug efflux, compounds predicted as potent inhibitors ...
Development of an assay for determining PDE5 enzyme activity and for screening of potential inhibitors of the PDE5 enzyme in cancer cells
(Master thesis; Mastergradsoppgave, 2013-05-15)
PDE5 is a member of the superfamily of phosphodiesterases, and it is identified as the main mechanism for breakdown of cGMP in mammals. Sildenafil is a well-known inhibitor of the PDE5 enzyme and it is also shown that sildenafil inhibits the ABCC5 transporter pump. ABCC5 is a member of the superfamily of ABC-transporters, and identified as an important transporter for mediating the cellular efflux ...
Hereditary breast and ovarian cancer. Diversity of genetic causes of HBOC in a Norwegian breast and ovarian cancer patient cohort, BRCA2 c.8331+2C>T - a Norwegian founder mutation
(Master thesis; Mastergradsoppgave, 2017-05-14)
Hereditary breast and ovarian cancer (HBOC) causes 5-10 % of breast cancer cases and 25 % of ovarian cancer cases. About 24 % of HBOC are caused by deleterious variants in BRCA1 and BRCA2. Currently, more than 25 different genes have been associated with HBOC, including BRCA1 and BRCA2, many of which encode proteins participating in homologous recombination repair (HRR) and mismatch repair (MMR). ...
Intracellular gelatinolytic activity in mouse tissues
(Master thesis; Mastergradsoppgave, 2011-09-12)
Matrix metalloproteinases have traditionally been thought to act only extracellulary. However, increasing evidence has revealed intracellular localization as well as several alternative substrates and novel biological roles for these proteinases. The aims of this master thesis were to map intracellular localization of MMP-2 and MMP-9 in different tissues from mouse, determine if detected enzymes ...
The antioxidant system of arctic seabirds and the effect of HOCs on antioxidant capacity of kidney tissue
(Master thesis; Mastergradsoppgave, 2010-06-15)
The arctic is exposed to a wide range of pollutants which originate from industrialized areas located in temperate regions. Halogenated organic contaminants (HOCs) are of special concern due to their persistency and ability to bioaccumulate. Consequently, animals high in the food chain, such as marine seabirds, are prone to accumulate high levels of these compounds. The HOCs are known to increase ...