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dc.contributor.authorAhmad, Azeem
dc.contributor.authorDubey, Vishesh Kumar
dc.contributor.authorButola, Ankit
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorMehta, Dalip Singh
dc.date.accessioned2020-11-29T09:51:26Z
dc.date.available2020-11-29T09:51:26Z
dc.date.issued2020-11-11
dc.description.abstract<p><i>Significance:</i> High temporal stability, wavelength independency, and scalable field of view (FOV) are the primary requirements of a quantitative phase microscopy (QPM) system. The high temporal stability of the system provides accurate measurement of minute membrane fluctuations of the biological cells that can be an indicator of disease diagnosis. <p><i>Aim:</i> The main aim of this work is to develop a high temporal stable technique that can accurately quantify the cell’s dynamics such as membrane fluctuations of human erythrocytes. Further, the technique should be capable of acquiring scalable FOV and resolution at multiple wavelengths to make it viable for various biological applications. <p><i>Approach:</i> We developed a single-element nearly common path, wavelength-independent, and scalable resolution/FOV QPM system to obtain temporally stable holograms/interferograms of the biological specimens. <p><i>Results:</i> With the proposed system, the temporal stability is obtained ∼15  mrad without using any vibration isolation table. The capability of the proposed system is first demonstrated on USAF resolution chart and polystyrene spheres (4.5-μm diameter). Further, the system is implemented for single shot, wavelength-independent quantitative phase imaging of human red blood cells (RBCs) with scalable resolution using color CCD camera. The membrane fluctuation of healthy human RBCs is also measured and was found to be around 47 nm. <p><i>Conclusions:</i> Contrary to its optical counterparts, the present system offers an energy efficient, cost effective, and simple way of generating object and reference beam for the development of common-path QPM. The present system provides the flexibility to the user to acquire multi-wavelength quantitative phase images at scalable FOV and resolution.en_US
dc.identifier.citationAhmad A, Dubey VK, Butola A, Ahluwalia BS, Mehta DS. Highly temporal stable, wavelength-independent, and scalable field-of-view common-path quantitative phase microscope. Journal of Biomedical Optics. 2020;25(11):116501en_US
dc.identifier.cristinIDFRIDAID 1848974
dc.identifier.doihttps://doi.org/10.1117/1.JBO.25.11.116501
dc.identifier.issn1083-3668
dc.identifier.issn1560-2281
dc.identifier.urihttps://hdl.handle.net/10037/19929
dc.language.isoengen_US
dc.publisherSPIE, the international society for optics and photonicsen_US
dc.relation.journalJournal of Biomedical Optics
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/288565/Norway/Integrated photonic chip-based nanoscopy for pathology & the clinic//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/285571/Norway/Optimalisering: High-throughput and high-resolution pathology using chip-based nanoscopy//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Technology: 500::Medical technology: 620en_US
dc.subjectVDP::Teknologi: 500::Medisinsk teknologi: 620en_US
dc.titleHighly temporal stable, wavelength-independent, and scalable field-of-view common-path quantitative phase microscopeen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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