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dc.contributor.authorTeubner, Marlene
dc.contributor.authorLenzen, Benjamin
dc.contributor.authorEspenberger, Lucas Bernal
dc.contributor.authorFuss, Janina
dc.contributor.authorNickelsen, Jörg
dc.contributor.authorKrause, Kirsten
dc.contributor.authorRuwe, Hannes
dc.contributor.authorSchmitz-Linneweber, Christian
dc.date.accessioned2020-12-23T13:08:17Z
dc.date.available2020-12-23T13:08:17Z
dc.date.issued2020-03-17
dc.description.abstractChloroplast RNAs are stabilized and processed by a multitude of nuclear-encoded RNA-binding proteins, often in response to external stimuli like light and temperature. A particularly interesting RNA-based regulation occurs with the <i>psbA</i> mRNA, which shows light-dependent translation. Recently, the chloroplast ribonucleoprotein CP33B was identified as a ligand of the <i>psbA</i> mRNA. We here characterized the interaction of CP33B with chloroplast RNAs in greater detail using a combination of RIP-chip, quantitative dot-blot, and RNA-Bind-n-Seq experiments. We demonstrate that CP33B prefers <i>psbA</i> over all other chloroplast RNAs and associates with the vast majority of the <i>psbA</i> transcript pool. The RNA sequence target motif, determined in vitro, does not fully explain CP33B’s preference for <i>psbA</i>, suggesting that there are other determinants of specificity in vivo.en_US
dc.identifier.citationTeubner M, Lenzen, Espenberger, Fuss J, Nickelsen J, Krause K, Ruwe, Schmitz-Linneweber C. The Chloroplast Ribonucleoprotein CP33B Quantitatively Binds the psbA mRNA. Plants. 2020;9(3)en_US
dc.identifier.cristinIDFRIDAID 1844701
dc.identifier.doi10.3390/plants9030367
dc.identifier.issn2223-7747
dc.identifier.urihttps://hdl.handle.net/10037/20138
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalPlants
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471en_US
dc.titleThe Chloroplast Ribonucleoprotein CP33B Quantitatively Binds the psbA mRNAen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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