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dc.contributor.authorLascu, Irina
dc.contributor.authorMereuță, Ioana
dc.contributor.authorChiciudean, Iulia
dc.contributor.authorHansen, Hilde
dc.contributor.authorAvramescu, Sorin Marius
dc.contributor.authorTănase, Ana-Maria
dc.contributor.authorStoica, Ileana
dc.date.accessioned2021-07-01T13:09:50Z
dc.date.available2021-07-01T13:09:50Z
dc.date.issued2021-05-01
dc.description.abstractPolyhydroxyalkanoates (PHAs) are biodegradable bioplastics that can be manufactured sustainably and represent a promising green alternative to petrochemical-based plastics. Here, we describe the complete genome of a new marine PHA-producing bacterium—Photobacterium ganghwense (strain C2.2), which we have isolated from the Black Sea seashore. This new isolate is psychrotolerant and accumulates PHA when glycerol is provided as the main carbon source. Transmission electron microscopy, specific staining with Nile Red visualized via epifluorescence microscopy and gas chromatography analysis confirmed the accumulation of PHA. This is the only PHA-producing Photobacterium for which we now have a complete genome sequence, allowing us to investigate the pathways for PHA production and other secondary metabolite synthesis pathways. The de novo assembly genome, obtained using open-source tools, comprises two chromosomes (3.5, 2 Mbp) and a megaplasmid (202 kbp). We identify the entire PHA synthesis gene cluster that encodes a class I PHA synthase, a phasin, a 3-ketothiolase, and an acetoacetyl-CoA reductase. No conventional PHA depolymerase was identified in strain C2.2, but a putative lipase with extracellular amorphous PHA depolymerase activity was annotated, suggesting that C2.2 is unable to degrade intracellular PHA. A complete pathway for the conversion of glycerol to acetyl-CoA was annotated, in accordance with its ability to convert glycerol to PHA. Several secondary metabolite biosynthetic gene clusters and a low number of genes involved in antibiotic resistance and virulence were also identified, indicating the strain's suitability for biotechnological applications.en_US
dc.identifier.citationLascu I, Mereuță, Chiciudean I, Hansen H, Avramescu SM, Tănase, Stoica I. Complete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidate. MicrobiologyOpen. 2021;10(2):1-16en_US
dc.identifier.cristinIDFRIDAID 1909457
dc.identifier.doi10.1002/mbo3.1182
dc.identifier.issn2045-8827
dc.identifier.urihttps://hdl.handle.net/10037/21661
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalMicrobiologyOpen
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Chemistry: 440en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Kjemi: 440en_US
dc.titleComplete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidateen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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