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dc.contributor.advisorJanssen, Kirstin
dc.contributor.advisorOlsen, Marita
dc.contributor.authorHamnevoll, Kari
dc.date.accessioned2021-07-02T06:35:49Z
dc.date.available2021-07-02T06:35:49Z
dc.date.issued2021-06-01en
dc.description.abstractmRNA profiling for body fluid or tissue type identification is able to provide circumstantial information alongside DNA profiles from biological material found at a crime scene. To solve the challenge of material limitation in body fluid identification, studies have successfully developed mRNA profiles of RNA extracted from lysis buffer discard of DNA-extraction. It would be interesting to examine whether this method is possible for other combinations of DNA- and RNA-isolation kits. Promising results were found for RNA quantity and quality measured in the DNA-extraction lysis buffer discard and DNA-eluate from body fluids extracted with the PrepFiler Express™ DNA Extraction system. The protocols of three RNA-isolation kits were optimized for extraction from Prepfiler™ lysis buffer discard, and the kits were compared in terms of RNA quantity and quality. The mirVana™ kit was found to be most suited for this purpose. Although the ReliaPrep™ RNA Miniprep kit had similar results for quantity and quality, a continuous problem was contamination of genomic DNA. The Direct-zol™ RNA Miniprep kit gave poor results and was not further examined. A preliminary singleplex of body fluid specific mRNA markers and housekeeping genes, using different PCR programs was performed on RNA extracted from pure body fluids, and products were separated by capillary electrophoresis. An overload of the system was observed for most of the body fluid markers. Primer tests should be rerun with less RNA, e.g. RNA extracted from lysis buffer discard instead of pure body fluids, before further optimization.en_US
dc.identifier.urihttps://hdl.handle.net/10037/21688
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universitetno
dc.publisherUiT The Arctic University of Norwayen
dc.rights.holderCopyright 2021 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en_US
dc.subject.courseIDMBI-3911
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.subjectRettsgenetikken_US
dc.subjectForensic Geneticsen_US
dc.subjectBody fluid identificationen_US
dc.subjectmRNA profilingen_US
dc.titleOptimization of the use of PrepFiler Express™ Forensic DNA extraction kit lysis buffer discard for mRNA profilingen_US
dc.typeMastergradsoppgaveno
dc.typeMaster thesisen


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Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)